Functional Comparison of Three Chitinases from Symbiotic Bacteria of Entomopathogenic Nematodes.

and bacterial symbionts of entomopathogenic nematodes and , respectively, have several biological activities including insecticidal and antimicrobial activities. Thus, XnChi, XhChi, and PtChi, chitinases of , , and isolated from Korean indigenous EPNs GJ1-2, GJ11-1, and GJ1-2 were cloned and expressed in BL21 to compare their biological activities. Chitinase proteins of these bacterial symbionts purified using the Ni-NTA system showed different chitobiosidase and endochitinase activities, but N-acetylglucosamidinase activities were not shown in the measuring of chitinolytic activity through N-acetyl-D-glucosarmine oligomers.