Publications by authors named "Getulio Pereira de Oliveira-Jr"
Article Synopsis
- Circulating RNAs, especially in extracellular vesicles (EVs) and non-EV carriers, have been studied for over two decades, but there's a lot of variability in results from samples, prompting a need for better standardization in how samples are collected and prepared.
- The study focuses on how different anticoagulants (ACs) affect the size, abundance, antigen makeup of circulating EVs, and specific circulating miRNA levels, which are important for disease detection in cancers and Alzheimer's disease.
- Findings indicate that while the number and size of plasma EVs vary with each AC, the overall antigenic composition remains mostly consistent; however, using EDTA as an AC was more effective for general miRNA levels, while
Extracellular vesicles (EVs) play a pivotal role in various biological pathways, such as immune responses and the progression of diseases, including cancer. However, it is challenging to isolate EVs at high purity from blood plasma and other biofluids due to their low abundance compared to more predominant biomolecular species such as lipoprotein particles and free protein complexes. Ultracentrifugation-based EV isolation, the current gold standard technique, cannot overcome this challenge due to the similar biophysical characteristics of such species.
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- * Different purification techniques for these EVs, like ExoQuick™, Total Isolation Kit, Ultracentrifugation, and Ultrafiltration, were compared to see how they affect the quality and characteristics of the isolated vesicles.
- * Findings revealed significant variations in the size, distribution, and protein content of the EVs based on the purification method used, highlighting the need for reliable protocols for clinical applications.
spp. are human pathogens that cause 181,000 deaths per year. In this work, we systematically investigated the virulence attributes of spp.
View Article and Find Full Text PDFNonlytic exocytosis is a process in which previously ingested microbes are expelled from host phagocytes with the concomitant survival of both cell types. This process has been observed in the interaction of spp. and other fungal cells with phagocytes as distant as mammalian, bird, and fish macrophages and ameboid predators.
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- Extracellular vesicles (EVs) are important for transferring biological information and disease markers, but identifying RNA in them is difficult.* -
- Molecular beacons (MBs) linked with cell-penetrating peptides (CPPs) can effectively deliver these beacons into cells to detect specific RNA molecules.* -
- The study found that MB-CPP can successfully detect a specific miRNA (miRNA-451a) in RBC-derived EVs using nano-flow cytometry, offering a new method to analyze RNA in EVs.*