Anthraquinones against : antifungal interaction, biofilm inhibition and pathogenicity in the model.

Cryptococcal biofilms have been associated with persistent infections and antifungal resistance. Therefore, strategies, such as the association of natural compounds and antifungal drugs, have been applied for the prevention of biofilm growth. Moreover, the pathogenicity model has been used to investigate the capacity to inhibit the pathogenicity of .

Antifungal Activity of Chitosan against in Planktonic and Biofilm Forms: A Therapeutic Strategy in the Future?

Histoplasmosis is a respiratory disease caused by , a dimorphic fungus, with high mortality and morbidity rates, especially in immunocompromised patients. Considering the small existing therapeutic arsenal, new treatment approaches are still required. Chitosan, a linear polysaccharide obtained from partial chitin deacetylation, has anti-inflammatory, antimicrobial, biocompatibility, biodegradability, and non-toxicity properties.

Inhibitory effect of proteinase K against dermatophyte biofilms: an alternative for increasing the antifungal effects of terbinafine and griseofulvin.

This study aimed to evaluate the effect of proteinase K on mature biofilms of dermatophytes, by assays of metabolic activity and biomass. In addition, the proteinase K-terbinafine and proteinase K-griseofulvin interactions against these biofilms were investigated by the checkerboard assay and scanning electron and confocal microscopy. The biofilms exposed to 32 µg ml of proteinase K had lower metabolic activity and biomass, by 39% and 38%, respectively.

The herbicide paraquat alters growth and melanin production on the species complex.

Paraquat (1,10-dimethyl-4,4-bipyridinium dichloride; PQ) is a free-radical producing herbicide that affects cell membranes and can upset the environmental balance of microorganisms present in soil, such as spp. This study aimed to evaluate the in vitro activity of PQ against spp. in planktonic and biofilm forms, as well as the protective effect of antioxidant agents against the antifungal effect of PQ and the kinetics of melanin production in response to PQ.

Anthraquinones from spp. inhibit : effects against growing and mature biofilms.

This study aimed to evaluate the effect of aloe emodin, barbaloin and chrysophanol on growing and mature biofilms of . The compounds were added at the moment of inducing biofilm growth or after growth for 72 h to evaluate their effects on growing and mature biofilms, respectively. Then, biofilm biomass was evaluated by crystal violet staining and metabolic activity by the XTT reduction assay.

Azole-Resilient Biofilms and Non-wild Type C. albicans Among Candida Species Isolated from Agricultural Soils Cultivated with Azole Fungicides: an Environmental Issue?

This study aimed to identify Candida spp. from agricultural soils cultivated with azole fungicides and investigate their susceptibility to clinical (fluconazole, itraconazole, voriconazole, and amphotericin B) and agricultural (tetraconazole and tebuconazole) antifungals in planktonic form. Additionally, Candida biofilm-forming ability and biofilm susceptibility to agricultural antifungals and voriconazole were analyzed.

Antifungal effect of anthraquinones against Cryptococcus neoformans: detection of synergism with amphotericin B.

The emergence of tolerant Cryptococcus neoformans strains to antifungals has been described. It has directed researchers to screen for new antimicrobial compounds. In this context, several plant-derived compounds, such as anthraquinones (aloe emodin, barbaloin, and chrysophanol), have been investigated for their antimicrobial properties.

and biofilms of dermatophytes: a new panorama for the study of antifungal drugs.

This study describes an model that creates an environment for dermatophyte biofilm growth, with features that resemble those of conditions, designing a new panorama for the study of antifungal susceptibility. Regarding planktonic susceptibility, MIC ranges were 0.125-1µg ml for griseofulvin and 0.

Darunavir inhibits / species complex growth and increases the susceptibility of biofilms to antifungal drugs.

species are pathogens commonly associated with cases of meningoencephalitis in individuals who are immunosuppressed due to AIDS. The aim was to evaluate the effects of the antiretroviral darunavir alone or associated with fluconazole, 5-flucytosine and amphotericin B against planktonic cells and biofilms of species. Susceptibility testing of darunavir and the common antifungals against 12 members of the / species complex was evaluated by broth microdilution.

inhibitory effect of statins on planktonic cells and biofilms of the species complex.

Sporotrichosis, caused by species of the complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. Statins are a class of drugs widely used for lowering high sterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of sterol. In this study, the antifungal activity of statins (simvastatin, atorvastatin, pravastatin) against planktonic cells and biofilms of complex species was evaluated, as well as the interaction of pravastatin with classical antifungals (amphotericin B, itraconazole, terbinafine).

biofilm-forming ability of dermatophytes using dog and cat hair: an ethically viable approach for an infection model.

The aim of this study was to establish an model for dermatophyte biofilm growth, using hair from dogs and cats. Strains of , , and were assessed for and biofilm production. All .

Malassezia pachydermatis from animals: Planktonic and biofilm antifungal susceptibility and its virulence arsenal.

The yeast Malassezia pachydermatis is a component of the microbiota of dogs and cats, however it can cause otitis and seborrheic dermatitis in these animals. The objective of this study was to determine the antifungal susceptibility, and evaluate virulence and pathogenicity of 25 M. pachydermatis strains from animals.

Candida parapsilosis complex in veterinary practice: A historical overview, biology, virulence attributes and antifungal susceptibility traits.

The Candida genus is composed by yeast that commensally live as part of human and animal microbiota. In the last years, C. parapsilosis complex, composed by the cryptic species C.