Implementing a biogeographic ancestry inference service for forensic casework.

The Centre of Forensic Sciences has validated the Precision ID Ancestry Panel on the Ion S5™ Massively Parallel Sequencing instrument for use in forensic casework. The focus of this paper is the development of reporting guidelines for implementation of the biogeographic ancestry inference service based on the Admixture Prediction results produced using the Torrent Suite™ Software (Thermo Fisher Scientific). The Admixture Prediction algorithm estimates the genetic ancestry of a sample using seven root populations (Europe, East Asia, Oceania, America, Africa, South Asia, and Southwest Asia).

Inferring the presence of spermatozoa in forensic samples based on male DNA fractionation following differential extraction.

To address sexual assault kit backlogs some laboratories in North America have implemented 'Direct to DNA' (DTD) approaches for the examination of relevant vaginal, oral, rectal and external genitalia swabs from sexual assault examination kits. Using this approach no preliminary serological screening for semen or spermatozoa is performed. Instead, swabs are directly subjected to differential extraction and quantitation using a dual quantification system.

Can a normal range of elbow movement predict a normal elbow x ray?

Background: Elbow injuries account for approximately 2-3% of presentations to the emergency department. This is associated not only with a very high rate of x rays but also with a very high rate of "missed fractures" This study examines which components of elbow examination have the best correlation with a normal radiograph.

Design Setting: A district general hospital's emergency department seeing 83,000 new attendances per annum (pa) (approximately 1600 elbow injuries pa).