A multiplex qPCR TaqMan-assay to detect fungal antagonism between (Hypocreaceae) and (Sclerotiniaceae) in blackberry fruits using a tef1-α- and an IGS-sequence based probes.

The aim of this study was to design a -specific qPCR oligo set, evaluate its specificity, and standardize a methodology that quantifies antagonism against in blackberry fruits ( Schltdl.). Primers and probe were designed based on the nuclear translation elongation factor 1-alpha (tef1-α) of .