Publications by authors named "German Madrigal-Redondo"
Cross comparison of alternative diagnostic protocols including substitution to the clinical sample, RNA extraction method and nucleic acid amplification technology for COVID-19 diagnosis.
Front Mol Biosci
August 2024
Background: the gold-standard diagnostic protocol (GSDP) for COVID-19 consists of a nasopharyngeal swab (NPS) sample processed through traditional RNA extraction (TRE) and amplified with retrotranscription quantitative polymerase chain reaction (RT-qPCR). Multiple alternatives were developed to decrease time/cost of GSDP, including alternative clinical samples, RNA extraction methods and nucleic acid amplification. Thus, we carried out a cross comparison of various alternatives methods against GSDP and each other.
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- Many labs use ELISA or CLIA tests to find anti-SARS-CoV-2 antibodies, but these methods require equipment that isn’t available in many low- and middle-income countries.
- A new dual antigen ELISA dot-blot test is introduced, offering high sensitivity and specificity to detect anti-SARS-CoV-2 IgG antibodies, and it can be done with minimal equipment in just one hour.
- This new test is also more affordable than existing rapid tests, making it a practical option for seroprevalence surveys in areas without access to advanced immunoassay facilities.
The main objective of this research is to develop an immediate release Rupatadine fumarate 10 mg tablets formulation by direct compression, through a Quality by Design approach in Costa Rica. According to a Quality by Design approach; Target Product Profile, Quality Target Product Profile, and the Critical Quality Attributes were defined. In the preformulation study, compatibility tests were carried out between the raw materials.
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