Bilateral rapidly destructive coxopathy in rheumatoid arthritis.

Rapidly destructive coxopathy (RDC) is a rare type of coxarthritis marked by swift deterioration of the hip joint. Although its cause remains unclear, several pathophysiological mechanisms are proposed. To comprehensively analyze this poorly understood condition, a literature search was conducted focusing on associations of bilateral RDC and rheumatoid arthritis (RA).

Vascular effects of biologic and targeted synthetic antirheumatic drugs approved for rheumatoid arthritis: a systematic review.

Background: Rheumatoid arthritis (RA) increases the risk of cardiovascular disease (CVD), with inflammation playing a key role. Biologic and targeted synthetic drugs used to treat RA can induce systemic immunomodulation and may have pleiotropic effects on vascular function, making it crucial to investigate their impact on CVD risk in RA patients.

Methods: A systematic review of the literature was conducted to investigate the impact of biologic and targeted synthetic treatments approved for RA on various cardiovascular markers, including endothelial function, arterial stiffness, and subclinical atherosclerosis.

Liquid scintillation counting of polycarbonates: a sensitive technique for measurement of activity concentration of some radioactive noble gases.

This work explores the application of the liquid scintillation counting of polycarbonates for measurement of the activity concentration of radioactive noble gases. Results from experimental studies of the method are presented. Potential applications in the monitoring of radioactive noble gases are discussed.

Influence of photon energy cuts on PET Monte Carlo simulation results.

Purpose: The purpose of this work is to study the influence of photon energy cuts on the results of positron emission tomography (PET) Monte Carlo (MC) simulations.

Methods: MC simulations of PET scans of a box phantom and the NEMA image quality phantom are performed for 32 photon energy cut values in the interval 0.3-350 keV using a well-validated numerical model of a PET scanner.

Replication of the pseudorabies virus in cultured pancreatic islet cells: further evidence of their paraneuronal nature.

Pancreatic B cells are known to be damaged by a wide range of viruses, causing diabetes. Though these viruses belong to different taxonomic groups, their single shared characteristic is neurotropism. In the present study, pseudorabies viral infection was modelled on fetal porcine islets cultivated in vitro.

[Characteristics of Newcastle virus isolated from live vaccine-immunized chickens--the unusual biological properties].

A strain of the Newcastle disease virus, denoted GF, was isolated from birds vaccinated twice with a live vaccine. In some flocks of the farm this strain has caused oligosymptomatic disease, and in others the infection has run a latent course. It was shown that the GF strain possessed unusual biologic properties--an extremely high virulence in vitro combined with a total lack of pathogenicity in vivo.

[Demonstration of a reovirus in helicopter disease of broilers].

A reovirus was isolated from 5-13-day-old broiler birds with signs of the so-called helicopter disease--one of the forms of the malabsorption syndrome. A number of organs were investigated (trachea, liver, spleen, bursa of Fabricius, proventricular stomach, and tendon sheath of musculus gastrocnemius). The virus was isolated from the proventricular stomach and the tendon sheath in cell cultures of chick embryo kidney as well as in 5-7-day-old chick embryos inoculated in the yolk sac.

[Precipitinogen activity of Newcastle disease virus and humoral immunity in birds].

Studies was the precipitinogenic activity of the strains La Sota, 'H', and 'II' of the Newcastle disease virus, using chick embryos. It was found that all three of the strains produce precipitinogens. An antigen was obtained for the precipitation reaction in agar gel and the demonstration of precipitins that were specific for the ND virus in the blood serum of birds.

[Differentiation of immunoglobulin classes in the chicken].

Investigations were carried out to establish and differentiate the humoral immunoglobulins in SPF-chickens, following an antigenic stimulus with Salmonella pullorum. As many as five components were found in the blood sera of the test birds, which were shown to have immunoglobulin properties. They were differentiated as IgM, IgA, IgG1, IgG2, and IgG3.

[Comparative study of tracheal organ cultures obtained from different bird donors].

Comparative studies were carried out on the quality (duration, intensity, and extent) of the cliliary activity in tracheal cultures from chick embryos, chickens, and pheasants. It was found that all cultures had long life, however, they were shown to differ essentially by the function of the tracheal epithelium. In tracheal cultures from pheasants the ciliary activity remained unchanged at the initial level for 70 days.

[Cultivation of various avian viruses in pheasant trachea organ cultures and chick embryos].

Stationary tracheal organ cultures of pheasants and chick embryos, treated with a mucolytic agent were used to study the ciliostatic effect of the following viruses: fowl pest (FPV), Newcastle disease (NDV), infections laryngotracheitis (ILV), and infections bronchitis (IBV) - strain Beaudette. In chick embryo tracheal cultures ciliostasis was found to set in as follows: for FPV - at the 24th hour; for NDV - at the 72nd-120th hour; for ILV - at the 168-192nd hour; and for IBV-at the 72nd-96th hour. The ciliostatic effect produced by NDV and IBV coincided in terms of time and dynamics.

[Serological response and resistance to Newcastle disease in vaccinated poultry].

Studies was the titer of antihemagglutinins of a total of 3263 serum samples taken from birds routinely vaccinated against Newcastle disease, originating from various poultry farms of the country. Chosen were 139 birds in which a weak serologic response was established through spray, aerosol, and injectable live vaccines (strain La Sota, Komarov). These birds were challenged by intramuscular application of 10(6) ELD50/bird of a velogenic viscerotropic virus of ND.

[Plaque cloning of the velogenic viscerotropic Newcastle disease virus].

A plaque clone was successfully produced and the plaque characteristic was studied of a velogenic viscerotropic Newcastle disease virus (strain II). The isolated 3 plaque mutants were found to form bright plaques in chick embryo fibroblast cultures, having a different size: those of clone I had a 4 mm dia, those of clone II - 2.5 mm dia, and those of clone III - 1.

[In vivo persistence of plaque mutants of viscerotropic Newcastle disease virus].

Studied was the persistance of 3 plaque clones of a velogenic viscerotropic Newcastle disease virus in nonimmune birds. It was found that the first plaque clone (4 mm dia of plaques) possessed higher virulence than that of the parental virus and killed all inoculated birds for 2 to 5 days. Plaque clone II (2.

[Establishing the immune status of poultry flocks against Newcastle disease in commercial poultry raising].

An experiment was carried on 133 grown up fowls and broilers from 4 industrial farms, vaccinated with spray lento and mesogenic vaccines for straining the immunity against pseodopest through RIHA and provoking pseudoplague with a pathogenic virus. A high degree of resistance was proved (94.8%), in spite of the fact that about 30% of the fowls investigated, showed low titres with regard to RIHA.

[Obtaining a specific antigen for the comparative testing of immunological methods in the diagnosis of Marek's disease].

Comparative studies were carried out on the diagnostic possibilities of immunological methods--precipitation in agar gel, immunofluorescence and indirect hemagglutination, as well as isolation of the virus through primary cellular cultures for the diagnosis of Marek's disease. For the purpose were obtained 9 series of specific antigens. The comparative studies were carried out on fowl and blood serums from different poultry yards, as well as from SPF chickens, experimentally infected with HPRS-16 strain.

[Pathogenic Newcastle virus carrier state in congenitally immune chickens].

Five-day-old chickens decendants of hens vaccinated several times under conditions existing in the practice were ophtalmo-nasally inoculated with 10(5),5ELD50 velogenic viscerothropic Newcastle Disease virus NDV. Thirty two per cent of them were stricken by the disease and died, but the remaining 68% resisted the provocation and showed no clinical symptoms 80 days post inoculation. The virulogical investigation of the chickens resistant to inoculation proved they are spreading the virus for 45 days and remained virus-carriers for 54 days post inoculation.

[Serological response and the viral carrier state in chickens infected with the pathogenic Newcastle disease virus].

Three groups of susceptible chickens were treated with Newcastle Disease vaccine, as follows: group I--orally, strain La Sota; group II--aerosole treatment, strain La Sota and group III--intramusculary, Komarov's vaccine. Varying HI antibody levels were observed following vaccine application. The average antibody titer of group I and II experimental chickens (40 in number for each group) was 25 days post vaccination respectively log2 = 3.

[Variability and the virulence of the Newcastle disease virus].

Six isolates of the Newcastle Disease virus (NDV), taken at different periods of time after provocation of immune birds with velogenous viscerotrophic strain, are used. It is shown that the virulence of the initial NDV challenge strain remains unchanges up the 46th day of persistence. The isolate, taken after 70 days of carriage, manifests a considerably decreased virulence.

[Cytopathic effect of the Newcastle disease virus on the BHK-21 cell line].

The cytopathic effect (CPE) of the Newcastle disease virus (NDV) in the BNK-21 permenant cell line is studied in a series of experiments. The CPE is assessed as a percentage of hemadsorption and polykaryocytosis, and the dynamics of the viral replication is followed up with the aid of the immunofluorescent method. The effect of the cyclohexamide protein inhibitor of the replication of various NDV strains, on the CPE, respectively, is likewise studied.

[Retention of the Newcastle disease virus in immunized poultry].

The period of virus harbouring was followed up in a total of 50 experimentally infected (Newcastle disease) birds. Two methodes were used to isolate the challenging virus: the ordinary virologic procedures and the method of tissue cultures. Studied was also the effect of the humoral immunity on the persistence of the virus.

[Role of the housefly in the epizootology of pseudoplague in birds].

It has been demonstrated that Musca domestica L. could harbour the Newcastle disease virus. It has been isolated from the surface of the flies body 96 hours after these had been in contact with it, and from their digestive tract--after 240 hours.