Comparison of Three CD3-Specific Separation Methods Leading to Labeled and Label-Free T Cells.

T cells are an essential part of the immune system. They determine the specificity of the immune response to foreign substances and, thus, help to protect the body from infections and cancer. Recently, T cells have gained much attention as promising tools in adoptive T cell transfer for cancer treatment.

Impact of high-energy electron irradiation on mechanical, structural and chemical properties of agarose hydrogels.

Due to their excellent biocompatibility and biodegradability, natural hydrogels are highly demanded biomaterials for biomedical applications such as wound dressing, tissue engineering, drug delivery or three dimensional cell culture. Highly energetic electron irradiation up to 10 MeV is a powerful and fast tool to sterilize and tailor the material's properties. In this study, electron radiation treatment of agarose hydrogels was investigated to evaluate radiation effects on physical, structural and chemical properties.

A comparative study of two separation methods to isolate monocytes.

Separation of specific blood cells is necessary for a deeper insight into their role in health and disease. To obtain such cells, efficient and robust isolation methods are needed. We compare here the Fab-based Traceless Affinity Cell Selection (TACS®) technology and the Magnetic Activated Cell Sorting (MACS®) technology to isolate human monocytes from whole blood and buffy coats as well as the differentiation of the isolated monocytes to dendritic cells (DCs).

[Biofunctional age diagnosis in humans. Potentials and limits].

The demand and requirements for valid, practicable, and reliable procedures for age diagnosis are increasing worldwide. In contrast, few studies and only a small number of procedures exist. The authors review the theoretical and methodological requirements for the development of models for age diagnostics.

Effect of different artificial aging conditions on ceramic-composite bond strength.

Purpose: The purpose of this study was to evaluate the influence of different storage conditions on the bond strength of adhesive bonding systems to yttria-partially stabilized zirconia ceramic.

Materials And Methods: Acrylic glass tubes filled with composite resin were bonded to ceramic disks. After sandblasting and ultrasonic cleansing of the ceramic samples, they were bonded using five bonding methods.

Pharmacokinetics of a synthetic, chemically modified hammerhead ribozyme against the rat cytochrome P-450 3A2 mRNA after single intravenous injections.

Modulation of gene expression via nucleic acid sequence-specific intervention represents a new paradigm for drug discovery and development. Ribozymes are small RNA structures capable of cleaving RNA target molecules in a catalytic fashion. A 2'-O-allyl-modified hammerhead ribozyme designed to cleave the messenger RNA of cytochrome P-450 3A2 was administered to rats via 0.

Survival-promoting and protein kinase C-regulating roles of basic FGF for hippocampal neurons exposed to phorbol ester, glutamate and ischaemia-like conditions.

Recent evidence suggests that protein kinase C (PKC) is involved in the pathophysiology of neurodegenerative diseases. We examined the effect of basic fibroblast growth factor (bFGF) on the survival of cultured rat hippocampal neurons exposed to conditions in which PKC is likely to play a role. bFGF reduced neuron damage caused by the PKC-activating phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), glutamate and ischaemia-like culture conditions.

Glutamate induces the growth factors NGF, bFGF, the receptor FGF-R1 and c-fos mRNA expression in rat astrocyte culture.

The effect of glutamate on primary cultures of rat cortical astrocytes was studied using Northern blot hybridization. Incubation with glutamate (100 microM, 15 min) induced nerve growth factor (NGF), basic fibroblast growth factor (bFGF), FGF receptor (FGF-R1) and proto-oncogene c-fos gene expression in a time dependent manner. Maximal induction of NGF, bFGF and FGF-R1 mRNA was reached after 4 h of incubation (7.

Opposite functions of jun-B and c-jun in growth regulation and neuronal differentiation.

Induction of the jun-B and/or c-jun transcription factors is part of the immediate early response to diverse stimuli that induce alterations in cellular programs. While c-jun is a protooncogene whose expression is required for induction of cell proliferation, jun-B has recently been found to be induced by stimuli inducing differentiation in various cell lines. Furthermore, its expression is largely restricted to differentiating cells during embryogenesis.

bFGF induces its own gene expression in astrocytic and hippocampal cell cultures.

Basic FGF mRNA induction by bFGF was investigated in cell cultures from rat brain, i.e. postnatal day 2 cortex and embryonic day 18 hippocampus.

Antisense bFGF oligodeoxynucleotides inhibit DNA synthesis of rat astrocytes.

Basic fibroblast growth factor (bFGF) is a multifunctional protein that stimulates proliferation, migration and differentiation of various cell types of the mammalian brain. In rat astrocyte cell cultures bFGF stimulates DNA synthesis after exogenous addition. This article will focus on the endogenous pathway of bFGF in rat astrocytes in cell cultures.