Seminoma in 46, XY Gonadal Dysgenesis: Rare Presentation and Review of the Literature.

Swyer syndrome is a rare congenital condition that serves as a risk factor for developing germ cell tumors. The condition belongs to the group of 46, XY disorders of sexual development, is characterized by complete gonadal dysgenesis (CGD) and is mostly manifested as delayed puberty and primary amenorrhea during adolescence. Individuals with Swyer syndrome are known to be phenotypically female with normal internal and external female genitalia at birth.

A de novo pathogenic variant identified in a boy with Poland syndrome.

Poland syndrome is a rare developmental disorder characterized by unilateral, complete or partial, absence of the pectoralis major (and often minor) muscle, accompanied with ipsilateral hand malformations. To date, no clear genetic cause has been associated with Poland syndrome, although familial cases have been reported. We report the employment of trio exome investigation and the identification of a heterozygous de novo pathogenic variant in the gene, a transcription factor associated with transcriptional repression during development, in a 14-yr-old boy with Poland syndrome.

C5aR agonist enhances phagocytosis of fibrillar and non-fibrillar Aβ amyloid and preserves memory in a mouse model of familial Alzheimer's disease.

According to the amyloid hypothesis of Alzheimer's disease (AD) the deposition of prefibrillar and fibrillar Aβ peptide sets off the pathogenic cascades of neuroinflammation and neurodegeneration that lead to synaptic and neuronal loss resulting in cognitive decline. Various approaches to reduce amyloid load by reducing production of the Aβ peptide or enhancing amyloid clearance by primary or secondary immunization have not proven successful in clinical trials. Interfering with the normal function of secretases and suboptimal timing of Aβ peptide removal have been put forward as possible explanations.

Endocrown restorations for extensively damaged posterior teeth: clinical performance of three cases.

The restoration of endodontically treated teeth (ETT) with more than one cusp missing and thin remaining walls is challenging for the general practitioner. The use of posts combined with full coverage restorations is a well-established approach, yet not following the minimal invasive principles of adhesive dentistry. Endocrowns are indirect monoblock restorations that use the pulp chamber of the ETT for retention.

Qualitative evaluation of hybrid layer formation using Er:YAG laser in QSP mode for tooth cavity preparations.

The purpose of this study was to evaluate the thickness and qualitative characteristics of the hybrid layer after two cavity preparation methods, using Er:YAG laser in QSP mode and conventional carbide burs. Additionally, two different adhesive techniques were investigated using etch-and-rinse and self-etch adhesive systems. Sixty sound human third molars were used and were randomly divided into four groups (n = 15).

Combined effect of glutamine at position 70 of HLA-DRB1 and alanine at position 57 of HLA-DQB1 in type 1 diabetes: An epitope analysis.

The contribution of specific HLA Class II alleles in type 1 diabetes is determined by polymorphic amino acid epitopes that direct antigen binding therefore, along with conventional allele frequency analysis, epitope analysis can provide important insights into disease susceptibility. We analyzed the highly heterogeneous Cypriot population for the HLA class II loci of T1DM patients and controls and we report for the first time their allele frequencies. Within our patient cohort we identified a subgroup that did not carry the DRB1*03:01-DQA1*05:01-DQB1*02:01 and DRB1*04:xx-DQA1*03:01-DQB1*03:02 risk haplotypes but a novel recombinant one, DRB1*04:XX-DQA1*03:01-DQB1*02:01 designated DR4-DQ2.

Effect of a calcium chloride solution treatment on physical and mechanical properties of glass ionomer cements.

This paper details a novel method to improve wear resistance of dental glass ionomer cement (GIC) restorations. The purpose of this in vitro study was to evaluate the effect of a calcium chloride (CaCl) solution treatment on surface hardness, surface roughness, compressive strength, water sorption and solubility of three conventional glass ionomer cements (GICs). Additionally, the changes of the Ca composition and morphology of the surface of the tested GICs were also investigated.

Effect of Three Clinical Curing Treatments on Fluoride Release and Surface Hardness of Glass-Ionomer Cements.

The purpose of this study was to investigate the effect of radiant heat, ultrasonic treatment, and 42.7 wt% CaCl₂ solution on fluoride release and surface hardness in three conventional glass-ionomer cements (GICs). The fluoride release patterns of each GIC were evaluated during a 28-day period using a fluoride ion-selective electrode.

Effect of Fluoride Treatments on Bleached Enamel Microhardness and Surface Morphology.

Purpose: To evaluate the effect of three different fluoride treatments on the surface microhardness and morphology of human enamel exposed to two different bleaching agents.

Materials And Methods: Twenty-three extracted human third molars were sectioned to obtain 90 enamel fragments, mounted on acrylic resin supports, polished and randomly divided into 9 groups (n = 10). The specimens were exposed to a combination of two bleaching agents (22% carbamide peroxide [CP] and 38% hydrogen peroxide [HP]) and three fluoride treatments (0.

Effect of radiant heat on conventional glass ionomer cements during setting by using a blue light diode laser system (445 nm).

The aim of this in vitro study was to evaluate the effect of radiant heat on surface hardness of three conventional glass ionomer cements (GICs) by using a blue diode laser system (445 nm) and a light-emitting diode (LED) unit. Additionally, the safety of the laser treatment was evaluated. Thirty disk-shaped specimens were prepared of each tested GIC (Equia Fil, Ketac Universal Aplicap and Riva Self Cure).

HLA-G variability and haplotypes detected by massively parallel sequencing procedures in the geographicaly distinct population samples of Brazil and Cyprus.

The HLA-G molecule presents immunomodulatory properties that might inhibit immune responses when interacting with specific Natural Killer and T cell receptors, such as KIR2DL4, ILT2 and ILT4. Thus, HLA-G might influence the outcome of situations in which fine immune system modulation is required, such as autoimmune diseases, transplants, cancer and pregnancy. The majority of the studies regarding the HLA-G gene variability so far was restricted to a specific gene segment (i.

Effect of radiant heat and ultrasound on fluoride release and surface hardness of glass ionomer cements.

Purpose: The purpose of this investigation was to evaluate the influence of radiant heat and ultrasound on fluoride release and surface hardness of 3 glass ionomer cements (GICs).

Methods: There were 3 experimental groups for each GIC; in group 1, the specimens were left to set without any treatment; in group 2, the specimens were irradiated for 2 minutes using a LED unit; and in group 3, ultrasound was applied using a scaler for 55 seconds on the specimen surface. Fluoride release measurements were performed daily for 7 days and at days 14 and 28.

Comparative evaluation of microleakage of a carbomer/fluoroapatite-enhanced glass-ionomer cement on primary teeth restorations.

Aim: Carbomer cement represents a novel glass-ionomer which gradually mineralises into fluoroapatite. Purpose of this study was to evaluate microleakage around restorations in deciduous teeth made with composite resin, conventional glass-ionomer cement, resin-modified glass-ionomer cement and carbomer/fluoroapatite-enhanced glass-ionomer cement.

Materials And Methods: A group of 40 primary upper canines, primary upper and lower molars was divided into 4 groups (n=10).

HLA-G 14-bp polymorphism affects the age of onset in Type I Diabetes Mellitus.

Type I diabetes mellitus (T1DM) is an organ-specific autoimmune disorder affecting the insulin-producing pancreatic cells. T1DM genetic association studies have so far revealed the involvement of more than 40 loci, with particularly strong associations for the human leucocyte antigens (HLA). Further to the well-established HLA class II associations, the immunomodulatory elements in the telomeric major histocompatibility complex locus, specifically nonclassical HLA class I, were also associated with T1DM, either in conferring susceptibility or by contributing to the overall pathogenesis.

Effect of preheating and shade on surface microhardness of silorane-based composites.

Aim: The aim of the present study was to evaluate the effect of preheating and shade on the surface microhardness of silorane-based composites.

Methods: Three shades of two different types of composites were evaluated: a silorane-based composite and a methacrylate-based composite. The composites were tested at 23°C, and after preheating at 55°C.

Viscoelastic properties, creep behavior and degree of conversion of bulk fill composite resins.

Objectives: The aim of this study was to investigate the viscoelastic properties and creep behavior of bulk fill composites under different conditions and evaluate their degree of conversion.

Methods: Seven bulk fill composites were examined: everX Posterior (EV), SDR (SD), SonicFill (SF), Tetric EvoCeram Bulk Fill (TE), Venus Bulk Fill (VE), x-tra base (XB) and x-tra fil (XF). Each material was tested at 21°C, 37°C and 50°C under dry and wet conditions by applying a constant torque for static and creep testing and dynamic torsional loading for dynamic testing.

cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit.

Background: The synthesis of complementary DNA (cDNA) for use in the detection of BCR-ABL1 at the Major Molecular Response (MMR) level is a well-established method used by clinical laboratories world-wide. However, the quality of cDNA provides sensitivity challenges and consequently affects the detection of Minimal Residual Disease (MRD).

Results: Herein, we evaluated six commercially available kits for the synthesis of cDNA according to amplification success rate, linearity and ABL1 copy number.

Comparison of elastomeric impression materials' thixotropic behavior.

The improved flow characteristics of new elastomeric impression materials are significant factors in the selection ofsuitableproductsfor clinical applications. The aim of this study was to assess the thixotropic behavior and compare the flow characteristics of seven different elastomeric impression materials using a shark fin test. One polyvinylsiloxane showed the highest shark fin height values, while the newly formed vinylsiloxanether material exhibited no significant differences when compared with two polyvinylsiloxanes.

Dynamic and static mechanical analysis of resin luting cements.

Various types of indirect restorations are available for dental treatment and resin cements are commonly used as a luting medium. The aim of this study was to evaluate the mechanical properties of contemporary resin luting agents under different testing conditions and temperatures. The materials tested were Choice 2 (CH), Clearfil Esthetic Cement (EC), Resicem (RC) and RelyX Unicem (RX).

The effect of home-use fluoride gels on glass-ionomer, compomer and composite resin restorations.

The purpose of this study was to investigate the resistance to dissolution by two home-use fluoride gels on the surface integrity of glass-ionomer, resin modified glass-ionomer, compomer and composite resin restorations. Class V cavities prepared in extracted teeth were restored with a glass-ionomer (Fuji II), a resin modified glass-ionomer (Vitremenr), two compomers (Dyract and F-2000) and a composite resin (Z-100). Groups of five specimens of each material were treated for 24 h with one of the following: (i).