It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed.
View Article and Find Full Text PDFCRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide sequence (protospacer) in the gene to be edited is important in the design of guide RNA, which can be carried out by specialized software. We review and compare all the known on-line and off-line resources for guide RNA design, with special attention paid to tools capable of searching for off-target edits sites in plant genomes.
View Article and Find Full Text PDFHeritable phenotypic alterations occurring during plant ontogenesis under the influence of environmental factors are among the most intriguing genetic phenomena. It was found that male-sterile sorghum hybrids in the 9E cytoplasm from the F1 and F2 generations, which were obtained by crossing CMS lines with different fertile lines grown in field conditions, were transferred to greenhouse produce fertile tillers. Lines created by the self-pollination of revertant tillers exhibit complete male fertility upon cultivation under various environments (in the field, Tdry plot,(y) Tirrigated plot(y)).
View Article and Find Full Text PDFThe most significant theories of the appearance and maintenance of sex are presented. However, in the overwhelming majority of existing theories, the problem of sex, which is the central problem of evolutionary biology, is considered primarily through the prism of reproductive features of living organisms, whereas the issue of molecular driving forces of sexual reproduction id restricted to the possible role of mobile genetic elements (MGEs) in the appearance of sexual reproduction. The structural and functional significance of MGEs in the genomic organization of plants is illustrated.
View Article and Find Full Text PDFEthidium bromide treatment (15 mg/l, 26 degrees C, 18 h) of a sorghum Zheltozernoe 10 callus culture yielded line Zh10-brl displaying multiple genetic instability. The line was characterized by a broad variety of mutations, which were identified in consecutive generations obtained from one initial regenerant via self-pollination. The mutation caused male sterility (male sterility, generation R1), a low plant height (dwarfness, R2), a reduced awn length (awnless, R3), yellow leaves in seedlings (xantha, R6), leaf variegation (leaf variegation, R6), leaf bleaching (virescence, R6), etc.
View Article and Find Full Text PDFThe effects of ubiquinone 50 (synthetic coenzyme Q10) and viral infection on phytohemagglutinin activity were studied in the tobacco (Nicotiana tabacum L.) cultivar Samsun NN, which carries the necrosis-formation locus and exhibits hypersensitivity to tobacco mosaic virus (TMV) and ubiquinone (Q10). The treatment with Q10 and inoculation with VTM were accompanied by an increase in phytohemagglutinin activity, indicating that lectins may be involved in the development of defense responses in plants.
View Article and Find Full Text PDFIzv Akad Nauk Ser Biol
December 2006
Hormonal system status has been analyzed in leaf disks of hypersensitive tobacco Nicotiana tabacum L. variety Samsun NN during the development of resistance to tobacco mosaic virus (TMV) induced by synthetic coenzyme Q10 (ubiquinone 50). The absolute and relative content of abscisic acid (ABA), indoleacetic acid (IAA), and cytokinins (CKs) was determined after the exposure of leaves to Q10 solution and the subsequent TMV infection.
View Article and Find Full Text PDFBased on RAPD-PCR analysis with 15 primers including those homologous to particular loci, the level of genetic polymorphism in the collection of spring common wheat genotypes and their androclinal double haploid (ADH) lines was determined. Intraspecific polymorphism of the original wheat forms was 20%. By the absence of polymorphism in the molecular patterns of ADH lines and original forms, it was shown that, under the conditions used, no genetic changes in the genomic DNA of spring common wheat in anther culture occurred.
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