Effect of microcystin-LR on protein phosphatase activity and glycogen content in isolated hepatocytes of fed and fasted juvenile goldfish Carassius auratus L.

Toxic effects of microcystin-LR were studied in hepatocytes isolated from fed and fasted juvenile goldfish Carassius auratus (30 g body weight). The hepatocytes were incubated with 10 microgMC-LR l(-1) during 4 h. MC-LR induces no effect in terms of cell number and viability.

Effect of microcystin-LR on protein phosphatase activity in fed and fasted juvenile goldfish Carassius auratus L.

Fed and fasted juvenile goldfish Carassius auratus (30 g body weight) were injected intraperitoneally (i.p.) with microcystin-LR (MC-LR) (125 microg/kg body weight) to determine the effect of alimentary status on the hepatic toxicity of MC-LR.

Hepatic accumulation and effects of microcystin-LR on juvenile goldfish Carassius auratus L.

After intraperitoneal injection of microcystin-LR (MC-LR) (125 microg kg(-1) body wt.), the concentration of MC-LR in the liver of juvenile goldfish Carassius auratus (30 g body wt.) was assayed by a modified protein phosphatase inhibition method.

Dopamine D1 receptors in the gills of Chinese crab Eriocheir sinensis.

Our investigations indicate the existence of binding sites for [3H]SCH23390 on crude membrane preparations in the anterior and posterior gills of the Chinese crab, Eriocheir sinensis acclimated to freshwater (FW) or seawater (SW). Maximum specific binding in the posterior gills is always higher than in the anterior gills, independent of saline acclimation. Kd values are similar in the two regions, suggesting the same affinity of both types of gills for the ligand, either from FW or from SW crabs.