Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH.

Siderophores are iron chelators produced by bacteria to access iron, an essential nutriment. Pyoverdine (PVDI), the major siderophore produced by Pseudomonas aeruginosa PAO1, consists of a fluorescent chromophore linked to an octapeptide. The ferric form of PVDI is transported from the extracellular environment into the periplasm by the outer membrane transporter, FpvA.

Isolation and activity of the promoters for STAT1 and 2 in Atlantic salmon Salmo salar.

Signal Transducer and Activator of Transcription (STAT) 1 and 2 molecules are part of the interferon (IFN) type I and type II (γIFN) signalling pathways, key pathways in the innate immune response. Genomic sequence regions upstream from the 5-prime Salmo salar ORFs were obtained and shown to have functional activity through their incorporation into luciferase reporter constructs and subsequent activation by salmonid alpha virus (SAV). The STAT1 and STAT2 putative promoter regions were also induced by co-transfected plasmids expressing γIFN and IFN type I respectively.

Aromatic thioglycoside inhibitors against the virulence factor LecA from Pseudomonas aeruginosa.

Three small families of hydrolytically stable thioaryl glycosides were prepared as inhibitors of the LecA (PA-IL) virulence factor corresponding to the carbohydrate binding lectin from the bacterial pathogen Pseudomonas aeruginosa. The monosaccharidic arylthio β-d-galactopyranosides served as a common template for the major series that was also substituted at the O-3 position. Arylthio disaccharides from lactose and from melibiose constituted the other two series members.

A lectin from Platypodium elegans with unusual specificity and affinity for asymmetric complex N-glycans.

Lectin activity with specificity for mannose and glucose has been detected in the seed of Platypodium elegans, a legume plant from the Dalbergieae tribe. The gene of Platypodium elegans lectin A has been cloned, and the resulting 261-amino acid protein belongs to the legume lectin family with similarity with Pterocarpus angolensis agglutinin from the same tribe. The recombinant lectin has been expressed in Escherichia coli and refolded from inclusion bodies.

Isolation and expression profile of a gene encoding for the Signal Transducer and Activator of Transcription STAT2 in Atlantic salmon (Salmo salar).

Signal Transducer and Activator of Transcription (STAT)-2 is a molecule involved in the type I interferon (IFN) signalling pathway. The full length cDNA sequence of Atlantic salmon (Salmo salar) ssSTAT2 was determined and phylogenetic analysis of the amino acid sequence grouped this novel salmon gene to the STAT2 clade. This represents the first fish STAT2 report.