Cryoimmobilized anther analysis reveals new ultrastructural insights into (Cyperaceae) asymmetrical microsporogenesis.

Introduction: The Cyperaceae family is distinguished by holocentric chromosomes and a distinctive microsporogenesis process, which includes inverted meiosis, asymmetric tetrad formation, selective cell death, and the formation of pseudomonad pollen. Despite significant advances, the ultrastructural details of these processes remain poorly understood.

Methods: This study provides a detailed analysis of microsporogenesis in using high-pressure freezing, freeze substitution, and transmission electron microscopy, significantly enhancing ultrastructural resolution.