Publications by authors named "Georgina C Gavins"
Templated chemistry offers the prospect of addressing specificity challenges occurring in bioconjugation reactions. Here, we show two peptide-templated amide-bond forming reactions that enable the concurrent labelling of two different membrane proteins with two different peptide nucleic acid () barcodes. The reaction system is based on the mutually selective coiled coil interaction between two thioester-linked -peptide conjugates and two cysteine peptides serving as genetically encoded peptide tags.
View Article and Find Full Text PDFArticle Synopsis
- - DNA nanotechnology is advancing the manipulation and imaging of proteins on cell surfaces, focusing on creating specific nucleic acid-protein junctions in living cells.
- - Researchers developed a rapid, covalent labeling method using a biostable peptide nucleic acid (PNA) tag that targets proteins with a specific coiled-coil peptide, allowing for the recruitment of fluorescent dyes.
- - The technique was successfully demonstrated on various cells and proteins, enabling versatile applications such as enhanced brightness with multiple fluorophores and real-time monitoring of protein internalization, specifically the epidermal growth factor receptor.
Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.
View Article and Find Full Text PDF