A Genomic-Based Workflow for eDNA Assay Development for a Critically Endangered Turtle, .

Environmental DNA (eDNA) analysis has become a popular conservation tool for detecting rare and elusive species. eDNA assays typically target mitochondrial DNA (mtDNA) due to its high copy number per cell and its ability to persist in the environment longer than nuclear DNA. Consequently, the development of eDNA assays has relied on mitochondrial reference sequences available in online databases, or in cases where such data are unavailable, de novo DNA extraction and sequencing of mtDNA.

History and historical DNA: Identity of Chelodina intergularis Fry, 1915 and type localities of C. intergularis and C. rugosa Ogilby, 1890.

Based on the phylogenetic placement of a near-complete mitogenome sequence of the holotype of Chelodina intergularis Fry, 1915 generated with hDNA approaches, we present evidence for the synonymy of this nominal species with Chelodina rugosa Ogilby, 1890. The type specimens of both taxa are housed in the Australian Museum, Sydney. Scrutinizing historical records, we conclude that the type locality of both taxa is most likely the vicinity of Somerset, at the northern extremity of Cape York Peninsula, Queensland, Australia.

NEUROPILIN-1 INHIBITION SUPPRESSES NERVE-GROWTH FACTOR SIGNALING AND NOCICEPTION IN PAIN MODELS.

Nerve growth factor (NGF) monoclonal antibodies inhibit chronic pain yet failed to gain approval due to worsened joint damage in osteoarthritis patients. We report that neuropilin-1 (NRP1) is a co-receptor for NGF and tropomyosin-related kinase A (TrkA) pain signaling. NRP1 was coexpressed with TrkA in human and mouse nociceptors.

Respective Roles of Electron-Phonon and Electron-Electron Interactions in the Transport and Quasiparticle Properties of SrVO_{3}.

The spectral and transport properties of strongly correlated metals, such as SrVO_{3} (SVO), are widely attributed to electron-electron (e-e) interactions, with lattice vibrations (phonons) playing a secondary role. Here, using first-principles electron-phonon (e-ph) and dynamical mean field theory calculations, we show that e-ph interactions play an essential role in SVO: they govern the electron scattering and resistivity in a wide temperature range down to 30 K, and induce an experimentally observed kink in the spectral function. In contrast, the e-e interactions control quasiparticle renormalization and low temperature transport, and enhance the e-ph coupling.

Variational benchmarks for quantum many-body problems.

The continued development of computational approaches to many-body ground-state problems in physics and chemistry calls for a consistent way to assess its overall progress. In this work, we introduce a metric of variational accuracy, the V-score, obtained from the variational energy and its variance. We provide an extensive curated dataset of variational calculations of many-body quantum systems, identifying cases where state-of-the-art numerical approaches show limited accuracy and future algorithms or computational platforms, such as quantum computing, could provide improved accuracy.

Repression by SNAIL Results in ECM Remodeling in Genetic Risk for Vascular Diseases.

Background: Genome-wide association studies implicate common genetic variations in the (low-density lipoprotein receptor-related protein 1 gene) locus at risk for multiple vascular diseases and traits. However, the underlying biological mechanisms are unknown.

Methods: Fine mapping analyses included Bayesian colocalization to identify the most likely causal variant.

Origin and fate of the pseudogap in the doped Hubbard model.

The relationship between the pseudogap and underlying ground-state phases has not yet been rigorously established. We investigated the doped two-dimensional Hubbard model at finite temperature using controlled diagrammatic Monte Carlo calculations, allowing for the computation of spectral properties in the infinite-size limit and with arbitrary momentum resolution. We found three distinct regimes as a function of doping and interaction strength: a weakly correlated metal, a correlated metal with strong interaction effects, and a pseudogap regime at low doping.

Beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy.

Beta-arrestins (βarrs) are key regulators and transducers of G-protein coupled receptor signaling; however, little is known of how βarrs communicate with their downstream effectors. Here, we use cryo-electron microscopy to elucidate how βarr1 recruits and activates non-receptor tyrosine kinase Src. βarr1 binds Src SH3 domain via two distinct sites: a polyproline site in the N-domain and a non-proline site in the central crest region.

Exact Numerical Solution of the Fully Connected Classical and Quantum Heisenberg Spin Glass.

We present the mean field solution of the quantum and classical Heisenberg spin glasses, using the combination of a high precision numerical solution of the Parisi full replica symmetry breaking equations and a continuous time quantum Monte Carlo algorithm. We characterize the spin glass order and its low-energy excitations down to zero temperature. The Heisenberg spin glass has a rougher energy landscape than its Ising analog, and exhibits a very slow temperature evolution of its dynamical properties.

Maternal diet influences fecundity in a freshwater turtle undergoing population decline.

Food availability determines the amount of energy animals can acquire and allocate to reproduction and other necessary functions. Female animals that are food limited thus experience reduced energy available for reproduction. When this occurs, females may reduce frequency of reproductive events or the number or size of offspring per reproductive bout.

Daily quetiapine after severe TBI improves learning and memory.

Background: Traumatic brain injury (TBI) induces cognitive deficits driven by neuroinflammation and cerebral edema. The commonly used atypical antipsychotic, quetiapine (QTP), has been recently shown to improve post-TBI outcomes. We hypothesized that QTP would thereby improve animal learning and memory 2 weeks after severe TBI.

Dose-Dependent Tranexamic Acid Blunting of Penumbral Leukocyte Mobilization and Blood-Brain Barrier Permeability Following Traumatic Brain Injury: An In Vivo Murine Study.

Background: Early posttraumatic brain injury (TBI) tranexamic acid (TXA) may reduce blood-brain barrier (BBB) permeability, but it is unclear if this effect is fixed regardless of dose. We hypothesized that post-TBI TXA demonstrates a dose-dependent reduction of in vivo penumbral leukocyte mobilization, BBB microvascular permeability, and enhancement of neuroclinical recovery.

Methods: CD1 male mice (n = 40) were randomly assigned to TBI by controlled cortical impact (injury [I]) or sham TBI (S), followed by intravenous bolus of either saline (placebo [P]) or TXA (15, 30, or 60 mg/kg).

Visualization of translation reorganization upon persistent ribosome collision stress in mammalian cells.

Aberrantly slow ribosomes incur collisions, a sentinel of stress that triggers quality control, signaling, and translation attenuation. Although each collision response has been studied in isolation, the net consequences of their collective actions in reshaping translation in cells is poorly understood. Here, we apply cryoelectron tomography to visualize the translation machinery in mammalian cells during persistent collision stress.

Fate of Quasiparticles at High Temperature in the Correlated Metal Sr_{2}RuO_{4}.

We study the temperature evolution of quasiparticles in the correlated metal Sr_{2}RuO_{4}. Our angle resolved photoemission data show that quasiparticles persist up to temperatures above 200 K, far beyond the Fermi liquid regime. Extracting the quasiparticle self-energy, we demonstrate that the quasiparticle residue Z increases with increasing temperature.

Thirty-day hospital readmission predictors in older patients receiving hospital-at-home: a 3-year retrospective study in France.

Objective: This study described older patients receiving hospitalisation-at-home (HaH) services and identified factors associated with 30-day hospital readmission.

Design: 3-year retrospective study in 2017-2019 in France.

Participants: 75 108 patients aged 75 years and older who were discharged from hospital medical wards (internal medicine and geriatric units) and admitted to HaH.

Neuropilin-1 is a co-receptor for Nerve Growth Factor-evoked pain.

Unlabelled: Nerve growth factor (NGF) monoclonal antibodies inhibit chronic pain yet, failed to gain approval due to worsened joint damage in osteoarthritis patients. We report that neuropilin-1 (NRP1) is a co-receptor for NGF and tropomyosin-related kinase A (TrkA) pain signaling. NRP1 is coexpressed with TrkA in human and mouse nociceptors.

Mott insulators with boundary zeros.

The topological classification of electronic band structures is based on symmetry properties of Bloch eigenstates of single-particle Hamiltonians. In parallel, topological field theory has opened the doors to the formulation and characterization of non-trivial phases of matter driven by strong electron-electron interaction. Even though important examples of topological Mott insulators have been constructed, the relevance of the underlying non-interacting band topology to the physics of the Mott phase has remained unexplored.

Distinct spin and orbital dynamics in SrRuO.

The unconventional superconductor SrRuO has long served as a benchmark for theories of correlated-electron materials. The determination of the superconducting pairing mechanism requires detailed experimental information on collective bosonic excitations as potential mediators of Cooper pairing. We have used Ru L-edge resonant inelastic x-ray scattering to obtain comprehensive maps of the electronic excitations of SrRuO over the entire Brillouin zone.