The chaperone PrsA2 regulates the secretion, stability, and folding of listeriolysin O during infection.

Unlabelled: Pathogenic bacteria rely on secreted virulence factors to cause disease in susceptible hosts. However, in Gram-positive bacteria, the mechanisms underlying secreted protein activation and regulation post-membrane translocation remain largely unknown. Using proteomics, we identified several proteins that are dependent on the secreted chaperone PrsA2.

The trophoblast surface becomes refractory to adhesion by congenitally transmitted and during cytotrophoblast to syncytiotrophoblast development.

The placenta is a critical barrier against viral, bacterial, and eukaryotic pathogens. For most teratogenic pathogens, the precise molecular mechanisms of placental resistance are still being unraveled. Given the importance of understanding these mechanisms and challenges in replicating trophoblast-pathogen interactions using models, we tested an existing stem-cell-derived model of trophoblast development for its relevance to infection with .

Prediction of post-hepatectomy liver failure by preoperative gadoxetate disodium-enhanced magnetic resonance imaging.

Background: Approximately 15% of patients experience post-hepatectomy liver failure after major hepatectomy. Poor hepatocyte uptake of gadoxetate disodium, a magnetic resonance imaging contrast agent, may be a predictor of post-hepatectomy liver failure.

Methods: A retrospective cohort study of patients undergoing major hepatectomy (≥3 segments) with a preoperative gadoxetate disodium-enhanced magnetic resonance imaging was conducted.

secretion chaperones PrsA, SlrA, and HtrA are required for competence, antibiotic resistance, colonization, and invasive disease.

is a Gram-positive bacterium and a significant health threat with the populations most at risk being children, the elderly, and the immuno-compromised. To colonize and transition into an invasive infectious organism, secretes virulence factors that are translocated across the bacterial membrane and destined for surface exposure, attachment to the cell wall, or secretion into the host. The surface exposed protein chaperones PrsA, SlrA, and HtrA facilitate protein secretion; however, the distinct roles contributed by each of these secretion chaperones have not been well defined.

Anesthesia and intraoperative high-frequency oscillatory ventilation during burn surgery.

High-frequency oscillatory ventilation (HFOV) is a mainstay in the ventilatory management of severe acute respiratory distress syndrome in our burn center. Many patients require burn surgery while on HFOV, potentially necessitating the use of HFOV during general anesthesia in the operating room. The purpose of this study was to describe the technique of providing and maintaining intraoperative HFOV.