Identification of NSDHL mutations associated with CHILD syndrome in oral verruciform xanthoma.

Objective: The aim of this study was to perform a systematic analysis of the nicotinamide adenine dinucleotide phosphate (NAD[P])-dependent steroid dehydrogenase-like (NSDHL) gene in cases of oral verruciform xanthoma (VX) and to test for the presence of mutations associated with congenital hemidysplasia with ichthyosiform nevus and limb defects (CHILD) syndrome.

Study Design: DNA was extracted from archived paraffin-embedded tissue of oral VX and control cases. Polymerase chain reaction (PCR) was then used to screen exons 4 and 6 of the NSDHL gene for the presence of 4 known germline mutations associated with CHILD syndrome and 1 somatic mutation previously identified in VX lesions with no known association with CHILD syndrome.

Bond strengths of a silorane composite to various substrates.

Purpose: To evaluate the shear bond strength (SBS) of a novel low-shrink posterior resin composite (Filtek LS) to various substrates.

Methods: The dedicated LS System Adhesive was used to bond Filtek LS to bovine dentin, ground bovine enamel, resin-modified glass-ionomer liner (Vitrebond Plus), conventional glass-ionomer restorative material (Fuji IX GP Extra), and bovine dentin previously exposed to zinc oxide-eugenol (IRM) (n=10 for each group). Vitrebond Plus and Fuji IX GP Extra substrates were fabricated by filling standardized preparations that had been made in epoxy resin.