Metatranscriptome and Resistome of the Endodontic Microbiome.

Introduction: In this study, we used metatranscriptomics for the first time to investigate microbial composition, functional signatures, and antimicrobial resistance gene expression in endodontic infections.

Methods: Root canal samples were collected from ten teeth, including five primary and five persistent/secondary endodontic infections. RNA from endodontic samples was extracted, and RNA sequencing was performed on a NovaSeq6000 system (Illumina).

Root canal curvature influences uncontrolled removal of dentin and cleaning efficacy after ultrasonic activation.

This study evaluated the correlation between root canal curvature and the effects of ultrasonic irrigation in the following parameters: volume of uncontrolled dentin removal (UDRVol), maximum depth of dentin defects, removal of accumulated hard tissue debris (AHTD), and canal transportation in prepared curved root canals. Twenty-four human permanent mandibular molars were divided into two groups according to root canal curvature: moderate curvature (MC: mean 25°); and severe curvature (SC: mean 48°). The specimens were scanned using an X-ray microcomputed scanner (Skyscan 1172) before and after cleaning and shaping and after the final irrigation protocol with ultrasonic irrigation.

Tissue repair capacity of bioceramic endodontic sealers in rat subcutaneous tissue.

This study aimed to evaluate the tissue repair capacity of four bioceramic endodontic sealers by quantifying type I and III collagen fibers. The following sealers were tested: EndoSequence BC Sealer (Brasseler, Brasseler, Savannah, USA), Bio C Sealer (Angelus, Londrina, Brazil), Bioroot RCS (Septodont, Santa Catarina, Brazil), and Sealer Plus BC (MKLife, Porto Alegre, Brazil). Polyethylene tubes 1.

Successful nonsurgical treatment of type II dens invaginatus with 5 root canals using a self-adjusting file: a case report.

The present report describes the endodontic treatment of an Oehlers type II dens invaginatus in a maxillary lateral incisor with 5 root canals, an extremely rare condition. Apical periodontitis and related symptoms were noted. Cone-beam computed tomography was used to aid the diagnosis, reveal tooth morphology, and assist in canal location.

Location of the Second Mesiobuccal Canal of Maxillary Molars in a Brazilian Subpopulation: Analyzing Using Cone-beam Computed Tomography.

Aim: This study aimed to evaluate the spatial location of the second mesiobuccal canal (MB2) of maxillary molars (MMs), using cone-beam computed tomography (CBCT), in a Brazilian subpopulation.

Materials And Methods: The CBCT examination of 250 patients performed on the Eagle 3D device was analyzed, totaling 787 MMs. Using the Radiant Dicom Viewer software, measurements were made of the distances, in millimeters (mm), between the first mesiobuccal canal (MB1), MB2, and palatal (P) canal inputs, from the axial sections.

Retrospective study of endodontic treatment performed by undergraduate students using reciprocating instrumentation and single-cone obturation.

Objectives: The aim of this cross-sectional retrospective study was to evaluate radiographically the technical quality of root canal treatment performed by undergraduate students using reciprocating NiTi instrumentation and single-cone obturation.

Methods: Endodontic treatment on 1102 teeth performed by undergraduate students from the School of Dentistry of the University of Santa Cecilia (Brazil) was evaluated. All root canal preparations were performed using Reciproc files and were irrigated with 1% NaOCl.

Repercussions of COVID-19 in Brazilian Dentists' Personal and Professional Routines: An Online Survey.

Aim And Objective: This study evaluated the repercussions of coronavirus disease-2019 (COVID-19) in Brazilian dentists' personal and professional routines.

Materials And Methods: Brazilian dentists were invited to participate in an online survey with questions pertaining to COVID-19 and its repercussions in dental practice. Sample calculation was performed using the Survey Monkey software (www.

Optimum glide path motion is safer than continuous rotation of files in glide path preparation.

This study evaluated the resistance to flexural fatigue and torsional strength of files for glide path preparation in continuous rotation or Optimum Glide Path motion (OGP). ScoutRace 15.02 and ProGlider 16.

Vertucci's Root Canal Configuration of 14,413 Mandibular Anterior Teeth in a Brazilian Population: A Prevalence Study Using Cone-beam Computed Tomography.

Introduction: This cone-beam computed tomographic (CBCT)-based study evaluated the root canal configurations of mandibular anterior teeth in a large Brazilian population, along with the influence of sex and the frequency of symmetry between the contralateral teeth.

Methods: Overall, 2543 CBCT scans, including those of 4773 lower central incisors (LCIs), 4835 lower lateral incisors (LLIs), and 4805 lower canines (LCs), were analyzed. The scans were obtained from a private radiologic clinic using the PreXion 3D device (Yoshida Dental Mfg Co, Ltd, Tokyo, Japan) with a 0.

Brazilian Primary and Secondary Public Oral Health Attention: Are Dentists Ready to Face the COVID-19 Pandemic?

Objective: To investigate knowledge and practice of Brazilian public primary and secondary health care dentists during the coronavirus disease (COVID-19) pandemic.

Methods: An online questionnaire with sociodemographic and COVID-19 knowledge questions was used.

Results: A total of 4048 dentists working in the Brazilian public primary and secondary health care system were investigated; 4024 (99.

Knowledge about Coronavirus disease 19 (COVID-19) and its professional repercussions among Brazilian endodontists.

The aim of this study was to assess Brazilian endodontists' level of knowledge about the coronavirus disease (COVID-19) and examine its professional repercussions. The link to the online survey that was created for this study was shared with Brazilian endodontists through social networking applications. The questionnaire contained questions that pertained to COVID-19 and its impact on dental practice.

Central giant cell granuloma mimicking a periapical lesion of endodontic origin: A case report.

This paper aims to report a case in which central giant cell granuloma (CGCG) mimicked a periapical lesion of endodontic origin. An 18-year-old female patient was referred for diagnosis and treatment of extensive radiolucent periapical lesion involving 31, 32, 33, 34 and 35 teeth. Clinically, the patient presented slight facial asymmetry and healthy teeth on the affected side with positive response to thermal vitality tests.

Torsional fatigue strength of reciprocating and rotary pathfinding instruments manufactured from different NiTi alloys.

To evaluate the torsional properties of engine-driven pathfinding instruments manufactured from different NiTi alloys - R-Pilot (tip size 12.5;.04 taper; M-Wire) and One G (tip size 14;.

Internal configuration of maxillary molars in a subpopulation of Brazil's Northeast region: A CBCT analysis.

The present study aimed to evaluate the internal configuration of the maxillary molars of a population from the Northeast region of Brazil. Cone-beam computed tomography (CBCT) exams from 512 patients (1501 teeth) were evaluated regarding the anatomical configuration of the root canal system, according to Vertucci's classification. The images were obtained using a Prexion 3D scanner operating at 90 kVp and 4 mA.

Durability of Two R-Phase Rotary Systems used in the Mechanical Instrumentation of Dilacerated Root Canals.

Aim: This study was aimed to analyze, through an test, the durability of two rotary systems used in curved root canals of extracted molars.

Materials And Methods: At first, 150 upper and lower molars were selected, presenting curvature at least in one of the root canals. They underwent access cavity and radiographed with a # 15 file into the canal to determine their maximum curvatures, classified as schneider.

Penetration of bioceramic and epoxy-resin endodontic cements into lateral canals.

The aim of the present study was to assess the penetration capacity of two endodontic cements, Endosequence BC Sealer and AH Plus, in artificial lateral canals. Twenty-six two-rooted, maxillary first premolars were instrumented to size 40.06 using K3 files.

Distance and Bone Density between the Root Apex and the Mandibular Canal: A Cone-beam Study of 9202 Roots from a Brazilian Population.

Introduction: Accidental damage to the inferior alveolar nerve is of concern during endodontic treatment of the posterior mandible teeth, and a careful evaluation of the relationship between the apices and the mandibular canal (MC) is mandatory to prevent iatrogenic complications. This study aimed to assess the distance between the root apices of posterior teeth and the MC and the bone density in such areas.

Methods: One thousand retrospective cone-beam computed tomographic scans were randomly selected from a private oral radiologic clinic database.

Nickel-titanium instruments in endodontics: a concise review of the state of the art.

The introduction of automated instrumentation in endodontics represented a major advance in progress for this specialty, with improvements in the quality and predictability of root canal preparation and a significant reduction in procedural errors. In recent years, endodontic instruments have undergone a series of changes brought about by modifications in design, surface treatments, and thermal treatments. In addition, new movements have also been incorporated to offer greater safety and efficiency, optimizing the properties of the NiTi alloy, especially through eccentric rotary motion.

Complex Apical Intraradicular Infection and Extraradicular Mineralized Biofilms as the Cause of Wet Canals and Treatment Failure: Report of 2 Cases.

This article describes 2 cases that showed persistent intracanal exudation (wet canal) even after several visits of antimicrobial endodontic treatment. Histologic and histobacteriologic investigation was conducted for determination of the cause. The 2 cases involved teeth with apical periodontitis lesions, which presented persistent exudation refractory to treatment after several visits.

Eight-year follow-up of autogenous tooth transplantation involving multidisciplinary treatment.

Although autogenous tooth transplantation is a widely reported procedure, its success is dependent on a number of factors. Here we describe the surgical technique, endodontic treatment and rehabilitation employed for a patient in whom a lower right third molar was transplanted to substitute an adjacent second molar with extensive caries. During an 8-year follow-up period, normal periodontal healing was observed and no infection, ankylosis or progressive resorption occurred.

Ex Vivo Evaluation of the Accuracy of Electronic Foramen Locators in Root Canals with an Obstructed Apical Foramen.

Introduction: The objective of the present study was to evaluate the accuracy of electronic foramen locators (EFLs), Root ZX II (RZX; J. Morita, Tokyo, Japan), Propex II (Dentsply Maillefer, Ballaigues, Switzerland), and Apex ID (AID; SybronEndo, Glendora, CA), in root canals with an obstructed apical foramen (OAF) and to compare them with those 1.0 mm short of the apical foramen (AF; -1.

RNA-based Assay Demonstrated Enterococcus faecalis Metabolic Activity after Chemomechanical Procedures.

Introduction: Because ribosomal RNA (rRNA) indicates metabolic cell activity, this study aimed to evaluate the sensitivity of rRNA-based quantitative polymerase chain reaction (RT-qPCR) for the identification of active Enterococcus faecalis in root canals samples compared with a method based on ribosomal DNA (rDNA) (rRNA genes).

Methods: Samples were taken from 18 teeth with persistent/secondary intraradicular infection before (S1) and after (S2) chemomechanical preparation. RNA and DNA were extracted, and complementary DNA was synthesized from RNA using RT-PCR.

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals.

The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days.

Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer.

Introduction: The aim of the present study was to evaluate the physicochemical properties of a bioceramic root canal sealer, Endosequence BC Sealer. Radiopacity, pH, release of calcium ions (Ca(2+)), and flow were analyzed, and the results were compared with AH Plus cement.

Methods: Radiopacity and flow were evaluated according to ISO 6876/2001 standards.

Resistance to flexural fatigue of Reciproc R25 files under continuous rotation and reciprocating movement.

Introduction: The aim of the present work was to evaluate the resistance to flexural fatigue of Reciproc R25 nickel-titanium files, 25 mm, used in continuous rotation motion or reciprocation motion, in dynamic assays device.

Methods: Thirty-six Reciproc R25 files were divided into 2 groups (n = 18) according to kinematics applied, continuous rotary (group CR) and reciprocation motion (group RM). The files were submitted to dynamic assays device moved by an electric engine with 300 rpm of speed that permitted the reproduction of pecking motion.