[Expression of cannabinoid receptor I during mice skin incised wound healing course].

Objective: To investigate the expression of cannabinoid receptor I (CB1R) during mice skin incised wound healing course and time-dependent changes of CB1R in wound age determination.

Methods: The changes of CBIR expression in skin incised wound were detected by immunohistochemistry and Western blotting.

Results: The control group showed a low expression of CB1R detected mainly in epidermis, hair follicles, sebaceous gland and dermomuscular layer.

[Expression of M3 subtype of muscarinic receptor during the skin incised wound healing in mice].

Objective: To investigate the expression of M3 subtype of muscarinic receptors (M3R) during the incised wound healing of the skin in mice and the characteristics of its time-dependent.

Methods: The change of M3R in skin incised wound was detected by immunohistochemical staining and Western blot.

Results: M3R-positive cells were detected in epidermis, hair follicle, sebaceous glands, sweat glands, dermomuscular layer in normal mouse skin.

[The time-dependent changes of phospho-JNK expression during the skin incised wound healing in mice].

Objective: To investigate the changes of phospho-JNK (p-JNK) during the incised wound healing of the skin in mice and to explore the rule of the time-dependent change of p-JNK in wound age determination.

Methods: The changes of p-JNK expression in incised skin wound were detected by immunohistochemistry and Western blot.

Results: There was a minimal baseline staining of p-JNK in control mouse skin.

[Animal model of grading skeletal muscle contusion due to blunt impact in rats].

Objective: To establish a new animal model of grading skeletal muscle contusions that could be controllable and repetitive.

Methods: The rats' gastrocnemius was injured by a new weight-dropping device designed. The force acting on gastrocnemius with a comparatively constant duration and inducing elastic deformation of the gastrocnemius was expressed with velocity (v) and deformation (DF).

[Immunohistochemical study on contents of beta-amyloid, alpha-actin and collagen IV in cerebral small vessels with unknown type of pathological lesion].

Objective: To investigate the structural characteristics of the cerebral small vessels with an unknown type of pathological lesion (UTPL).

Methods: Contents of beta-amyloid, alpha-actin and collagen IV in cerebral small vessels with UTPL were studied by Congo red staining, immunohistochemical staining and computer image analysis.

Results: The low expression levels of alpha-actin and collagen IV (P<0.