[Establishment of malignant progression associated gene expression profiles in human brain glioma].

Objective: To establish malignant progression associated gene expression profiles in human brain glioma.

Methods: The primary (WHO grade II), recurrent (WHO grade III) and re-recurrent (WHO grade IV) glioma specimens were sequentially collected from one single patient. Gene expression of different tumor specimens and normal brain tissue of the same patient was compared by microarrary techniques.

Small envelope protein E of SARS: cloning, expression, purification, CD determination, and bioinformatics analysis.

Aim: To obtain the pure sample of SARS small envelope E protein (SARS E protein), study its properties and analyze its possible functions.

Methods: The plasmid of SARS E protein was constructed by the polymerase chain reaction (PCR), and the protein was expressed in the E coli strain. The secondary structure feature of the protein was determined by circular dichroism (CD) technique.

Profiling genes differentially expressed in NGX6 overexpressed nasopharyngeal carcinoma cells by cDNA array.

Purpose: To investigate the role of the NGX6 gene in carcinoma proliferation and profile the downstream genes regulated by NGX6 in a nasopharyngeal carcinoma (NPC) cell line.

Methods: We established a NPC cell line with NGX6 overexpression by gene transfection. Subsequently, a high-density cDNA array was used to identify differentially expressed genes in NGX6-overxepressed cells.