Analysis of the Amine Submetabolome Using Novel Isotope-Coded Pyrylium Salt Derivatization and LC-MS: Herbs and Cancer Tissues as Cases.

The amine submetabolome, including amino acids (AAs) and biogenic amines (BAs), is a class of small molecular compounds exhibiting important physiological activities. Here, a new pyrylium salt named 6,7-dimethoxy-3-methyl isochromenylium tetrafluoroborate ([]-DMMIC) with stable isotope-labeled reagents ([]-/[]-DMMIC) was designed and synthesized for amino compounds. []-/[]-/[]-DMMIC-derivatized had a charged tag and formed a set of molecular ions with an increase of 3.