Exploration and Practice of the "One Combination, Two Highlights, Three Combinations, Four in One" Innovative Talents Training Mode in Forensic Medicine.

Talent is one of the basic and strategic supports for building a modern socialist country in all aspects. Since the 1980s, the establishment of forensic medicine major and the cultivation of innovative talents in forensic medicine have become hot topics in higher education in forensic medicine. Over the past 43 years, the forensic medicine team of Shanxi Medical University has adhered to the joint education of public security and colleges, and made collaborative innovation, forming a training mode of "One Combination, Two Highlights, Three Combinations, Four in One" for innovative talents in forensic medicine.

[Cathepsin L expression in plasma after acute myocardial ischemia and ischemia-reperfusion in rats].

Objective: To test cathepsin L as a biomarker of myocardial ischemia by examination of cathepsin L expression in plasma after myocardial ischemia and ischemia-reperfusion in rats.

Methods: The rat models were established and divided in acute myocardial ischemia model (myocardial ischemia 30 min, 1 h, 2 h groups), ischemia-reperfusion model (ischemia-reperfusion group), and isoflurane-pretreated ischemia-reperfusion model (isoflurane-pretreated group), respectively. Normal control group and sham-operated group were established as contrast.

Sphingosine-1-phosphate receptors respond differently to early myocardial ischemia and ischemia-reperfusion in vivo.

Sphingosine-1-phosphate (S1P) has been demonstrated to be a mediator and marker of heart diseases. We hypothesized that the expression of S1P receptors is involved in the S1P-mediated cardioprotection in vivo and may serve as a biomarker of ischemic heart disease. In vivo models of myocardial ischemia (MI) and ischemia-reperfusion (IR) were established by ligation of the left anterior descending artery (LAD) of rat heart, the mRNA expressions of S1PR1-3 were detected using real time PCR at different time intervals after ischemia (LAD for 15 min, 30 min, and 1 h) and IR.

[Genetic polymorphism of interleukin-2 gene in Chinese Han and Thai populations].

Objective: To investigate the polymorphisms of interleukin-2 (IL-2) gene at position -330 in the promoter region and +114 in the first exon, and to study the polymorphism distribution difference between the Chinese Han and Thai ethnic groups.

Methods: Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, authors detected the single nucleotide polymorphisms at position -330T/G and +114G/T in IL-2 genes of 130 healthy Chinese Han and 105 healthy Thai individuals.

Results: The frequency of TT genotype in IL-2 gene at position -330 was 36.

Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus.

Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several human malignancies including B lymphomas and nasopharyngeal carcinoma. The EBV R transactivator (Rta) has been found to play essential roles in stimulating a lytic cycle and viral gene expression. Recently, it was shown that ELISA detecting serum IgG-Rta(150+185) (two internal fragments of Rta) levels may be useful as a serological parameter to assist in the diagnosis of nasopharyngeal carcinoma.

[Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models].

Objective: To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia.

Methods: Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques.

[Expression of HIF1-alpha on myocardium and lung in rats model of asphyxia death].

Objective: To investigate the expression of HIF1-alpha in heart and lung tissue died from asphyxia.

Methods: The rats model of asphyxia death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time (0,2,6,24 h), immunohistochemistry and half-quantitative RT-PCR methods were used to investigate expression of HIF1-alpha and mRNA changes on heart and lung tissue.

Results: The positive staining of HIF1-alpha could be observed in the myocardium and lung tissue.

[Study of polymorphism at new Y-STR DYS605 in a Chinese Han population of Shanxi].

We study the polymorphism at DYS605 ,a new tetranucleotide Y-STR locus,in a Chinese Han population of Shanxi to meet the need of more genetic markers in forensic practice and genetic analysis. DNA were extracted from 128 unrelated male venous blood, and amplified using GDB primers. PCR products were detected using non-denaturing polyacrylamide gel electrophoresis and silver staining.