The application of a polymerase chain reaction (PCR)-based capillary electrophoretic technique provides detailed insights into Eimeria populations in intensive poultry establishments.

Intestinal coccidiosis, caused by one or multiple species of Eimeria (Protozoa: Apicomplexa), is one of the most important infectious diseases affecting chickens. In this study, we used a polymerase chain reaction (PCR)-based capillary electrophoresis (CE) approach to conduct an epidemiological survey of Eimeria species in seven Australian broiler flocks, varying in age from 18 to 42 days. We confirmed that all seven recognized Eimeria species of poultry were present.

Investigating a persistent coccidiosis problem on a commercial broiler-breeder farm utilising PCR-coupled capillary electrophoresis.

In the present study, we utilised a polymerase-chain-reaction-coupled capillary electrophoresis (CE) approach to investigate the epidemiology of Eimeria species on a broiler-breeder farm in Victoria, Australia. The Eimeria populations of two flocks vaccinated against coccidiosis were followed over an 11-week period. All seven recognised Eimeria species of chickens were detected in both flocks.