Datasets on the nutritional and environmental (including biodiversity) characteristics of food products consumed in France.

Analysing the nutritional and environmental impacts of our current diets and promoting sustainable dietary shifts require quantified data on the characteristics of foods. We have jointly studied environmental and nutritional performances of more than 200 generic foods consumed in France, by combining and completing different databases. Several environmental issues calculated by Life Cycle Assessment (LCA) were selected, including impacts on biodiversity.

Notch3 regulates Mybl2 via HeyL to limit proliferation and tumor initiation in breast cancer.

Notch signaling is a conserved signaling pathway that participates in many aspects of mammary gland development and homeostasis, and has extensively been associated with breast tumorigenesis. Here, to unravel the as yet debated role of Notch3 in breast cancer development, we investigated its expression in human breast cancer samples and effects of its loss in mice. Notch3 expression was very weak in breast cancer cells and was associated with good patient prognosis.

Contactin-1 is a novel target antigen in membranous nephropathy associated with chronic inflammatory demyelinating polyneuropathy.

Primary membranous nephropathy (MN) is an autoimmune glomerular disease in which autoantibodies are directed against podocyte proteins. In about 80% of cases the main targeted antigen is the phospholipase A2 receptor 1 (PLA2R1). Anti-PLA2R1 antibodies are mainly immunoglobulin G type 4 (IgG4).

'A sword of Damocles': patient and caregiver beliefs, attitudes and perspectives on presymptomatic testing for autosomal dominant polycystic kidney disease: a focus group study.

Background And Objectives: Presymptomatic testing is available for early diagnosis of hereditary autosomal dominant polycystic kidney disease (ADPKD). However, the complex ethical and psychosocial implications can make decision-making challenging and require an understanding of patients' values, goals and priorities. This study aims to describe patient and caregiver beliefs and expectations regarding presymptomatic testing for ADPKD.

Range and Variability of Outcomes Reported in Randomized Trials Conducted in Patients With Polycystic Kidney Disease: A Systematic Review.

Rationale & Objective: Trials in autosomal dominant polycystic kidney disease (ADPKD) have increased, but their impact on decision making has been limited. Because heterogeneity in reported outcomes may be responsible, we assessed their range and variability in ADPKD trials.

Study Design: Systematic review.

Identifying patient-important outcomes in polycystic kidney disease: An international nominal group technique study.

Aim: Patients with autosomal dominant polycystic kidney disease (ADPKD) are at increased risk of premature mortality, morbidities and complications, which severely impair quality of life. However, patient-centered outcomes are not consistently reported in trials in ADPKD, which can limit shared decision-making. We aimed to identify outcomes important to patients and caregivers and the reasons for their priorities.

Characterization of the binding mode of JNK-interacting protein 1 (JIP1) to kinesin-light chain 1 (KLC1).

JIP1 was first identified as scaffold protein for the MAP kinase JNK and is a cargo protein for the kinesin1 molecular motor. JIP1 plays significant and broad roles in neurons, mainly as a regulator of kinesin1-dependent transport, and is associated with human pathologies such as cancer and Alzheimer disease. JIP1 is specifically recruited by the kinesin-light chain 1 (KLC1) of kinesin1, but the details of this interaction are not yet fully elucidated.

Cell Density Affects the Detection of Chk1 Target Engagement by the Selective Inhibitor V158411.

Understanding drug target engagement and the relationship to downstream pharmacology is critical for drug discovery. Here we have evaluated target engagement of Chk1 by the small-molecule inhibitor V158411 using two different target engagement methods (autophosphorylation and cellular thermal shift assay [CETSA]). Target engagement measured by these methods was subsequently related to Chk1 inhibitor-dependent pharmacology.

Development and validation of a rapid HPLC method for the determination of oseltamivir phosphate in Tamiflu and generic versions.

Oseltamivir phosphate (OP) is an antiviral drug that is used in the treatment and prophylaxis of both influenza A and influenza B. It is effective against all known influenza viruses than can infect humans, including pandemic influenza viruses and may be the most appropriate antiviral option against avian influenza caused by H5N1 virus. Tamiflu, the registered trademark used under exclusive license by Roche laboratories with OP as active pharmaceutical ingredient, is considered the best treatment for the bird flu disease.

Low blood pressure and risk of depression in the elderly. A prospective community-based study.

Background: The relationship between depression and low blood pressure in unclear.

Aims: To examine the temporal relation between low blood pressure and depression in a two-year follow-up.

Method: The study group consisted of 1389 subjects aged 59-71 years; 1272 (92%) were examined after two years.

[Sports activities and declared health status].

A cross-section study on sports and health was carried out among 8666 subjects from 15 to 49 years old, for a large range of practices. The analysis compared three groups, 2048 non-athletics, 5381 "moderately" athletic, and 1237 "highly" athletic. Compared to non-athletics, the "moderately" athletic group has a higher opinion of their state of health, with a similar frequency of declared health problems (hospitalisations, absences from work, traumatisms).

Both Fc alpha domains of human IgA are involved in in vitro interaction between secretory component and dimeric IgA.

The sites of interaction between 125I labelled human secretory component (SC) and dimeric IgA were located by studying the inhibitory effect of various antibodies to IgA. Several Fab' fragments were isolated from three sera of hyperimmunized rabbits. The specificity of these different antibody preparations, as determined by a RIA inhibition test or by ELISA, showed that two were directed against both domains of Fc alpha, two against C alpha 2, two against C alpha 3 and one against Fd alpha.

Human secretory component. IV: Antigenic regions involved in in vitro binding to dimeric IgA.

The aim of this report was to identify the region(s) of the secretory component (SC) molecule involved in in vitro binding to dimeric IgA. Inhibition of the SC binding was tested by Fab' antibody fragments directed against the accessible (A) and inaccessible (I) regions of SC. Antibodies directed against the main 38.

Differences between the in vitro combinations of secretory component (SC) and immunoglobulin polymers (Ig) by enumeration of SC epitopes.

The enumeration of the SC epitopes has been established on 125I-labelled free and combined SC, by binding to anti-SC coated beads, then by addition of 3H-labelled anti-SC Fab' fragments of various specificities. The number of moles of Fab' fragments found on the beads increases in relation to the introduced amount. The extrapolation to an infinite concentration of added Fab' fragments gives the maximal theoretical accessible number of SC epitopes.

Analysis of the vaccinating group specificity a of HBsAg with monoclonal antibodies. Identification of continuous and discontinuous epitopes.

The group specificity a of HBsAg is responsible for the induction of protective antibodies. Several synthetic peptides for vaccination are in preparation. It is not known if the structure of the epitope(s) implied in specificity a is continuous or discontinuous.

Enumeration of the antigenic determinants of human secretory component (SC) and secretory IgA (sIgA).

The number of antigenic determinants of human SC and sIgA was determined with specific anti-SC antibodies isolated from a pool of two hyperimmunized sheep. The Fab' antibody fragments were prepared and [125I] labelled, while pure SC and pure sIgA, isolated from colostrum, were labelled with 131I. The [125I] Fab' antibodies were added, in very large molar excess, to the [131I] antigens at various ratios.

Human secretory component. II. Easy detection of abnormal amounts of combined secretory component in human sera.

A simple method, allowing easy detection of abnormally increased sIgA levels is described. It consists in quantitation of combined SC by gel double diffusion, using appropriate anti-SC immune sera. The technical conditions, locating the threshold of sensitivity of precipitation at about 25 microgram/ml, a value higher than that found in normal sera, were established.