[Activity of glutamate dehydrogenase, gamma-glutamyltranspeptidase and creatine kinase in saliva in gingivitis].

In the human saliva (oral fluid), the activity of glutamate dehydrogenase tends to decrease in gingivitis and diminishes in periodontitis. That of gamma-glutamyl transpeptidase and creatine kinase increases in gingivitis. In periodontitis, their activity is higher than that in gingivitis.

[Iron in oral cavity fluid in gingival inflammation].

In parodontosis, iron levels are higher in the human gingival fluid and mixed saliva. The free iron-binding capacity of saliva is also increased. In gingivitis, higher levels of iron and free binding capacity are less evident.

[Adenylate cyclase and guanylate cyclase in the saliva of healthy persons and in periodontitis].

Studies of mixed salivary pools of normal subjects aged 22-35 and of patients with periodontitis of slight and medium degree, aged 25-55, have shown a 1.5 times lower activity of salivary adenylate cyclase in the patients with slight periodontitis as against normal subjects and an almost two times lower activity of this enzyme in medium-severity periodontitis patients as against those with the slight condition. The directions of changes in guanylate cyclase activities are contrary; this enzyme activity is elevated in the patients with the slight-degree condition vs.

[Effects of saccharose diet and drinking water with different mineral composition on calcium-phosphorus metabolism in the teeth and bones of rats].

Metabolism of 32P and 45Ca in the teeth and bones was studied in 140 Wistar rats that received a high-saccharose diet and varying amounts of Ca, Mg and F in drinking water. The water hardness and F content influence 45Ca and 32P metabolism in the teeth and bones and caries development. 45Ca and 32P inclusion into mineralized tissues was decreased in rats fed with the high-saccharose diet as compared to the control animals which received a usual ration.

[The effect of magnesium sulfate electrophoresis and galvanization on the mineralization of teeth and bones].

Effects of Mg2+ electrophoresis and galvanization on tooth and bone mineralization was experimentally studied with the use of radioactive Ca and P isotopes. Mg2+ electrophoresis and, to a lesser degree, galvanization enhanced 32P incorporation in incisors and maxillary bones. Mg2+ significantly increased 45Ca incorporation in teeth and maxillary bones.

[The effect of local exposure to Mg and heat on the biosynthesis of sulfur-containing compounds in the oral tissues].

Local Mg electrophoresis was shown to considerably increase the radiolabeled sulphate contents in an application area: gums, mandibular alveolar processes, lower incisors and molars roots. In areas far beyond the phoresis sites (femoral epiphyses and diaphyses) label incorporation was not affected. Mg-free galvanization had no effect on sulphate incorporation into the mineralized tissues.

[Iododeoxyuridine metabolism in the oral tissues and its alteration in infection by the herpes simplex virus].

In healthy white rats [125I]-desoxyuridine, [3H]-thymidine, 2-[14C]-uracyl, 8-[14C]-adenine and [3H)-uridine were used to demonstrate that the [125I]-desoxyuridine metabolism in oral mucosa is analogous to that of thymidine and different from metabolic pathways of another nucleoside uridine and nitrogen purine and pyrimidine bases of adenine and uracyl. In rats infected with herpes simplex virus, the relative tissue/blood 123I radioactivity increased after a [125I]-desoxyuridine injection. The authors suggest that the mechanism of [125I]-desoxyuridine action relies on its involvement into the viral host cell DNA.

[Correction of mineral and protein metabolism by anabolic preparations and vitamin D2 in rats with dental caries].

The effect of nerabol, namacyte and vitamin D2 introduction (separately or in combination) on the animal mineral and protein metabolism has been studied in the experiment on 210 white Wistar rats with reproduced caries. Biochemical and radioisotopic investigations have permitted determining the quantitative content of phosphorus, calcium, 14C-glycine in the saliva, blood serum, teeth, jaw bones and femur. The data obtained evidence for the pronounced caries-static effect of the tested preparations connected with their active influence on the mineral and protein metabolism of experimental animals as well as for the expediency to use the above drugs for complex prophylaxis and treatment of dental caries normalizing processes.

[Age-related characteristics of the effect of deafferentation of the mandible on the metabolism of sulfate, citrate, phosphorus and calcium in the bones].

Mineral metabolism in the bones of 1- and 3-month rats reacts to a different extent to cutting the lower alveolar nerve. After deafferentation of the lower jaw, the decrease in the intensity of incorporation of the intraperitoneally injected [3-14C]-citrate and [35S]-sulphate in the bones is more significant in 1-month animals as compared to that in older ones. Deafferentation increased utilization of Na2H32PO4 and 45CaCl2 in the bones of younger rats, but decreased incorporation of 32P and remained unchanged incorporation of 45Ca in 3-month animals.

[Age-related changes in ATPase activity from rat salivary glands].

The ATP hydrolysis by homogenates of oral cavity mucosa, parotid, mandibular and sublingual glands of rats was studied depending on the animal age (1,3 and 12 months). In all the studied tissues the souring of incubation medium due to ATP hydrolysis is followed by its subsequent alkalization up to the initial pH. The alkalization rate increases with the animal age and by 12 months reaches its constant value (within 22.

[Role of salivary ribonuclease in pathogenesis of periodontosis].

In patients with periodontal disease a distinct increase in secretion of salivary acidic and alkaline RNAases was found. The RNAases activity in mixed saliva correlated with the intensity of degradation of periodontal tissues during loss of alveolar layer in aged Wistar rats and the spontaneous periodontal disease in cats. In experiments with golden hamsters salivary RNAase was found to penetrate through mucosa in active form.

[Role of salivary kallikrein in the pathogenesis of periodontosis].

In patients with parodontosis the distinct increase in salivary kallikrein secretion was found. In Wistar rats the intensity of age atrophy of alveoli dentalis correlated with BAEE-esterase activity of mixed saliva. Desalivation considerably decreased the rate of age atrophy of parodontosis in rats.

[Effect of prodigiozan on the course of candidiasis of the oral mucosa clinically and in an experiment].

The effect of various doses of prodigiozan on Candida infection was studied under experimental and clinical conditions. Hamsters and guinea pigs were used in the experiments. Prodigiozan was administered intramuscularly to the hamsters in doses of 100 gamma/kg of the body weight 1 hour before the infection and intracutaneously to the guinea pigs in doses of 50 gamma/kg 2 days before the infection.