Identification and analgesic activity study of analgesic protein Ⅶ-2 from venom.

Background: Acid-sensing ion channel 1a (ASIC1a) plays a critical role in physiological and pathological processes. To further elucidate the biological functions of ASICs and their relationships with disease occurrence and development, it is advantageous to investigate and develop additional regulatory factors for ASICs.

Methods: In this study, cation exchange chromatography was used to separate seven chromatographic components from venom.

Hydroxyl radical dominated ibuprofen degradation by UV/percarbonate process: Response surface methodology optimization, toxicity, and cost evaluation.

Ibuprofen (IBP) is a typical nonsteroidal anti-inflammatory drug with a wide range of applications, large dosages, and environmental durability. Therefore, ultraviolet-activated sodium percarbonate (UV/SPC) technology was developed for IBP degradation. The results showed that IBP could be efficiently removed using UV/SPC.

Performance Prediction of Cement Stabilized Soil Incorporating Solid Waste and Propylene Fiber.

Cement stabilized soil (CSS) yields wide application as a routine cementitious material due to cost-effectiveness. However, the mechanical strength of CSS impedes development. This research assesses the feasible combined enhancement of unconfined compressive strength (UCS) and flexural strength (FS) of construction and demolition (C&D) waste, polypropylene fiber, and sodium sulfate.

An Additive Consistency and Consensus Approach for Group Decision Making With Probabilistic Hesitant Fuzzy Linguistic Preference Relations and Its Application in Failure Criticality Analysis.

In this article, probabilistic hesitant fuzzy linguistic preference relations (PHFLPRs) are proposed to present the qualitative pairwise preference information of decision makers (DMs) with hesitation and probability uncertainty assessments. The measurements and improvements of additive consistency and consensus of PHFLPRs are investigated in group decision making (GDM). First, a new concept of probabilistic hesitant fuzzy linguistic term sets is defined.

[Application of micellar electrokinetic capillary chromatography for identification, quantitative detection and unfolding analysis of interleukin-12].

Objective: To establish a micellar electrokinetic capillary chromatography-based method for identification and quantitative detection of interleukin-12 (IL-12) and analysis of its unfolding process.

Methods: An uncoated fused-silica capillary (inner diameter 50 μm) with a total length of 48.5 cm (40 cm to the detector) was used for the experiment.

Purification and characterization of Protein C activator from Venom.

Protein C (PC) plays an important role in the balance of coagulation and anticoagulation. Thus, the detection of PC activity is diagnostically significant for patients with cardiovascular diseases. Presently, the key methods to detect PC activity are the chromogenic assay and activated partial thromboplastin time (APTT) test.

Multi-Objective Optimization for Grinding Parameters of 20CrMnTiH Gear with Ceramic Microcrystalline Corundum.

(1) The alloy material 20CrMnTiH is widely used in gear manufacturing, but difficult to process, and its quantity (efficiency) and quality (surface quality) are generally negative correlation indicators. As a difficult but realistic problem, it is of important practical significance to explore how to efficiently grind high-precision low-carbon alloy gear workpieces. (2) Firstly, the pixel method was applied to analyze the grinding principles and explore the grinding parameters-the grinding wheel speed and grinding wheel frame moving speed-as well as the feed rate, which impacts the grinding indicators.

[Knockdown of LSD1 promotes differentiation of human induced pluripotent stem cells into insulin-producing cells in vitro].

Objective To investigate a protocol for the efficient differentiation of human induced pluripotent stem cells (hiPSCs) into insulin-producing cells (IPCs) in vitro. Methods Lysine-specific demethylase 1 (LSD1) gene was knocked down in hiPSCs by RNAi. A four-step method was performed to induce the differentiation of hiPSCs into IPCs.

[Effect of Agkistrodon Acutus Venom PCA on HUVEC Activity].

Objective: To investigate the effects of Agkistrodon acutus venom protein C activator(PCA) on ultrastructure of human umbilical vein endothelial cells(HUVEC), and the levels of tissue factor(TF), vascular von Willebrand factor (vWF) and endothelin-1 secreted by HUVEC and to clarify the anti-thrombotic mechanism of PCA.

Methods: The experiments were divided into control group(DMEM), LPS group (LPS 0.1 µg/ml), PCA group(PCA 1 µg/ml) and PCA+LPS group (1 µg/ml PCA+ 0.

Agkistrodon acutus-purified protein C activator protects human umbilical vein endothelial cells against HO-induced apoptosis.

Context: Recent studies show that the Agkistrodon acutus (Viperidae) (syn. Deinagkistrodon acutus) protein C activator (PCA) treats acute myocardial infarction and ischaemia-reperfusion animal models effectively, while the underlying mechanism remains unknown.

Objective: To study the effect of PCA on the injury of human umbilical vein endothelial cells (HUVECs) induced by HO and the underlying mechanism.

[Effect of AAVC-I on the migration of human umbilical vein endothelial cells].

Objective: To investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I.

Methods: The effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations: 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay.

A new Agkistrodon halys venom-purified protein C activator prevents myocardial fibrosis in diabetic rats.

Aim: To assess the effects of protein C activator (PCA) from Agkistrodon halys snake venom on cardiac fibrosis in streptozotocin (STZ) induced diabetic rat model, and investigate the mechanisms of its action.

Methods: PCA was identified by one-dimensional reversed phase liquid chromatography - mass spectrometry/mass spectrometry. Male Sprague-Dawley rats (120-140 g) were randomly assigned to negative control (NC) and diabetic group.

[Expressions of fractalkine and CD11c on common carotid artery atherosclerotic plaques from apoE(-/-) mice].

Objective: To explore the association of fractalkine (FKN) and CD11c expressions oncommon carotid artery atherosclerotic plaques from apoE(-/-) mice with the severity of atherosclerotic lesions.

Methods: Totally 24 apoE(-/-) mice were divided into two groups and fed on a high-fat diet or a normal diet for 12 weeks. Then the blood lipids as well as the plaque area and vascular stenosis rate of the common carotid artery were measured to evaluate the severity of atherosclerotic lesions of the animals.

[Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1].

This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment.

[Application of capillary zone electrophoresis in the interaction analysis of protein C with protein C activator from Agkistrodon acutus venom].

A new capillary zone electrophoresis method (CZE) has been established for the interaction analysis of protein C (PC) with a protein C activator (PCA) from Agkistrodon acutus venom. The analysis was performed on an uncoated fused-silica capillary with 75 microm i.d.

[Component I from Agkistrodon acutus venom induces apoptosis of K562/A02 cells by promoting caspase 3 expression].

To investigate the effects of component I from Agkistrodon acutus venom (AAVC-I) on the biological features of chronic myeloid leukemia cells, K562/A02 leukemia cells were cultured in the presence of AAVC-I (6.25 - 100 µg/ml) and the proliferation status was assayed by CCK-8 method. Morphological changes were observed by inversed microscope after Giemsa and Hochest 33258 staining, and cell apoptosis was detected by flow cytometry.

[Apoptosis of K562 cells induced by extract of Agkistrodon Halys' venom].

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase (ERK) in K562 cells was detected by Western blot. The results showed that when K562 cells were treated with 0, 1, 10, 20 microg/ml of the extraction for 48 hours, the apoptosis rates were 2.

Endoxin-mediated myocardial ischemia reperfusion injury in rats in vitro.

Myocardial ischemia reperfusion results in an increase in intracellular sodium concentration, which secondarily increases intracellular calcium via Na(+)-Ca2+ exchange, resulting in cellular injury. Endoxin is an endogenous medium of digitalis receptor and can remarkably inhibit Na+/K(+)-ATPase activity. Although the level of plasma endoxin is significantly higher during myocardial ischemia, its practical significance is unclear.