Improved succinate production by metabolic engineering.

Succinate is a promising chemical which has wide applications and can be produced by biological route. The history of the biosuccinate production shows that the joint effort of different metabolic engineering approaches brings successful results. In order to enhance the succinate production, multiple metabolical strategies have been sought.

Effects of pH and dissolved CO2 level on simultaneous production of 2,3-butanediol and succinic acid using Klebsiella pneumoniae.

The influences of pH and dissolved CO2 level on the regulation of growth and formation of catabolic end products have been investigated in Klebsiella pneumoniae. With increasing CO2 levels, there were no apparent changes in 2,3-butanediol production but succinic acid productions were enhanced significantly. A novel strategy for co-production of 2,3-butanediol and succinic acid using K.

Soluble expression of active human beta-defensin-3 in Escherichia coli and its effects on the growth of host cells.

Background: Human beta-defensin-3 (HBD(3)) is an epithelial peptide that has been demonstrated to have a salt-insensitive broad spectrum of potent antimicrobial activity. Expressing antimicrobial peptides in Escherichia coli (E. coli) is very difficult for it can result in death of the bacterial host cells.