Clonality analysis of neuroendocrine cells in gastric adenocarcinoma.

Aim: To achieve a better understanding of the origination of neuroendocrine (NE) cells in gastric adenocarcinoma.

Methods: In this study, 120 cases of gastric adenocarcinoma were obtained. First, frozen section-immunohistochemistrical samples were selected from a large quantity of neuroendocrine cells.

[Detection and clinicopathologic significance of microsatellite alteration and p53 mutation of neuroendocrine cells in colorectal adenocarcinoma].

Objective: To study the possible clonal origin of neuroendocrine cells in colorectal adenocarcinoma.

Methods: Twenty-six microsatellite loci were screened using laser capture microdissection, DNA extraction and whole genome amplification. Microsatellite instability (MSI) and loss of heterozygosity (LOH) in adenocarcinoma cells and neuroendocrine cells amongst 30 cases of colorectal carcinoma with neuroendocrine differentiation were detected using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)-silver staining.

[Regulation and mechanism of Notch signaling pathway in small cell lung cancer].

Objective: To investigate the status of Notch signaling pathway in small cell lung cancer (SCLC).

Methods: Expression plasmids of pEFBOS-NIC-MYC and pEFBOS-neo were transfected into NCI-H446 cells. Stably transfected cell lines were selected and their growth rates were examined by MTT method.

[Expression and significance of CD147 and E-cadherin in human gastric carcinoma].

Objective: To investigate the expression of CD147 and E-cadherin in gastric carcinoma and their correlation with clinicopathological features.

Methods: The expression of CD147 and E-cadherin in gastric cancer tissue chip (TC) was detected by in situ hybridization (ISH) and immunohistochemistry in 220 cases of gastric carcinoma and 31 cases with normal gastric mucosa.

Results: The expression rates of CD147 mRNA, E-cadherin mRNA and E-cadherin protein were 50.

[Inhibitory effects of Notch1 overexpression on proliferation and neuroendocrine marker expression in a small cell lung cancer cell line].

Objective: To investigate the effects of Notch1 signal activation on proliferation and neuroendocrine marker expression in small cell lung cancer cells.

Methods: The active form of Notch1 (NIC) was over-expressed in NCI-H446 cells by constitutive transfection and a stable transfected cell line was established. Proliferation of NCI-H446 cells was analysed by MTT assay on 6 successive days.

[Study on the expression and significance of Galectin-3 and CDC25B mRNA in human gastric carcinoma].

Objective: To study the expression of Galectin-3 and CDC25B mRNA in gastric carcinoma and their correlation with clinical-pathological features and the survival time.

Methods: Tissue microarray (TMA) technique and in situ hybridization were used to detect the expression of Galectin-3 and CDC25B mRNA in 220 gastric carcinoma specimens and 31 normal gastric mucosa samples.

Results: In situ hybridization results revealed that from the 220 cases, the positive expression rate of Galectin-3 and CDC25B mRNA were 58.

[Microsatellite instability and allele-specific chromosome 3p deletion in breast cancer and precancerous lesions].

Objective: To investigate the incidence and clinicopathologic significance of MSI and LOH on 3P in breast carcinoma and its precancerous lesions, intraductal papillary adenoma and ductal carcinoma in situ.

Methods: 41 paired sporadic invasive breast carcinomas, 13 archival precancerous lesion specimens of the breast and 14 couples of benign hyperplasia were collected. Twelve microsatellites on chromosomes 2p, 3p, 5q, 6q, 16q, 17q, eleven markers on chromosome 3p were amplified for MSI and LOH, respectively, by polymerase chain reaction ( PCR ) with designed primers and detecting after polyacrylamide gel electrophoresis.

[Loss of heterozygosity on chromosome 3p in breast cancer and precancerous lesion].

Objective: To study the loss of heterozygosity (LOH) on chromosome 3p in breast cancers and precancerous lesion.

Methods: LOH at 11 microsatellite loci was detected in 41 cases of breast cancers and 12 cases of precancerous lesion by polymerase chain reaction and silver stain. The expressions of ER, PR, FHIT and hMLH1 were detected in breast cancer by immunohistochemistry.

Correlative studies on bFGF mRNA and MMP-9 mRNA expressions with microvascular density, progression, and prognosis of gastric carcinomas.

Aim: To investigate the mRNA expressions of bFGF and MMP-9 in gastric carcinomas so as to reveal their correlations with tumor microvascular density (MVD), invasion, metastasis, and prognosis.

Methods: In situ hybridization and immunohistochemical techniques were used to detect the expressions of bFGFmRNA and MMP-9mRNA and the proteins of CD34 in 105 specimens of gastric carcinomas.

Results: In situ hybridization study showed that positive rates of bFGF mRNA and MMP-9mRNA expressions were 60.

[Relationship between bFGF mRNA and MMP-9 mRNA expression in gastric carcinoma and their clinicopathological features as well as patients survival].

Objective: To investigate the mRNA expression of bFGF and MMP-9 in gastric carcinomas and to find their correlation with tumor microvascular density (MVD), invasion, metastasis and patients survival.

Methods: In situ hybridization and immunohistochemistry technique were used to test the expression of bFGF mRNA and MMP-9 mRNA and protein of CD34 in 105 specimens of gastric carcinoma.

Results: In situ hybridization revealed that the positive rates of bFGF mRNA and MMP-9 mRNA were 60.

Biological characteristics of breast carcinomas with neuroendocrine cell differentiation.

Background: The aim of this study was to investigate DNA content and expression of c-erbB-2, PS2, and prostate-specific antigen (PSA) proteins in breast carcinomas with neuroendocrine (NE) cell differentiation.

Methods: Chromogranin, c-erbB-2, PS2, and PSA in 131 samples of breast cancer were detected immunohistochemically. Classic Feulgen staining image analysis techniques were used to quantify DNA content in 81 of the breast cancer samples.

Neuroendocrine markers in adenocarcinomas: an investigation of 356 cases.

Aim: To investigate the incidence of neuroendocrine (NE) cells and their hormone products in adenocarcinomas and evaluate their significance in clinical pathology and prognosis.

Methods: By using tissue sectioning and immunocyto-chemistry, 356 cases of adenocarcinomas were studied to examine the presence of chromorgranin and polypeptide hormones in adenocarcinoma samples from our hospital.

Results: The positive rate of NE cells and hormone products was 41.

[Study of neuroendocrine markers in 356 cases of adenocarcinomas].

OBJECTIVE: To ivestigate the incidence of neuroendocrine (NE) cells and hormone products in adenocarcinomas and to explore its clinicopathological significance. METHODS: 356 cases of adenocarcinomas were studied by immunocytochemistry with antibodies for chromorgranin and polypeptide hormones. RESULTS: The prevalence of NE cells and hormone products were detected in 54 of 130(41.