Developmental Times of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) at Constant Temperatures and Applications in Forensic Entomology.

The characteristic life stages of infesting blowflies (Calliphoridae) such as Chrysomya megacephala (Fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. We determined the developmental rates of C. megacephala from southwest China at seven constant temperatures (16-34°C).

[Low volume amplification in single cell separation and inspection].

Objective: To establish optimal amplification conditions with the application of 0.2 mL test tube in single cell separation and inspection.

Methods: Oral epithelium cell suspension was prepared.