Zhongguo Zhong Yao Za Zhi
December 2016
Objective: To evaluate the quality coherence of the prepared slices of Polygoni Cuspidati Rhizoma commercially available in China and provide a reference for their quality evaluation.
Methods: The fingerprints were obtained using HPLC method, and analyzed with Chromatographic Fingerprint Similarity Evaluation System (2004A Version) provided by Chinese Pharmacopoeia Commission. The experiment was carried out with an Agilent TC-C18 column (250 mm x 4.
Objective: To analyze the volatile oil from Radix Euphorbia Pekinensis.
Methods: Volatile oil was extracted from Radix Euphorbia Pekinensis by steam distillation and GC-MS was employed for detecting the content of the constituents. The relative content of the chemical constituents were calculated using area normalization method.
Objective: To reveal the correlation between HQT gene and the biosynthesis of chlorogenic acid in Lonicera japonica.
Methods: RT-PCR was used to measure the relative expression of HQT gene and reference gene Actin, and agarose gel electrophoresis was used to analyse the PCR results.
Results: The brightness of Actin gene strips of different organs was properly similar to each other,but the brightness of HQT gene strips was significantly different.
Zhongguo Zhong Yao Za Zhi
March 2005
Objective: To explore the genetic basis of using three species of Asarum as Herba Asari to determine the taxonomic positions of Asarum heterotropoides and A. siebodii; and to apply DNA molecular analysis as a tool for identification of Herba Asari.
Method: PCR, purification, sequence analysis were prerformed.