Enhanced radiation use efficiency and grain filling rate as the main drivers of grain yield genetic gains in the CIMMYT elite spring wheat yield trial.

Common wheat (Triticum aestivum L.) is a major staple food crop, providing a fifth of food calories and proteins to the world's human population. Despite the impressive growth in global wheat production in recent decades, further increases in grain yield are required to meet future demands.

Night-time warming in the field reduces nocturnal stomatal conductance and grain yield but does not alter daytime physiological responses.

Global nocturnal temperatures are rising more rapidly than daytime temperatures and have a large effect on crop productivity. In particular, stomatal conductance at night (g ) is surprisingly poorly understood and has not been investigated despite constituting a significant proportion of overall canopy water loss. Here, we present the results of 3 yr of field data using 12 spring Triticum aestivum genotypes which were grown in NW Mexico and subjected to an artificial increase in night-time temperatures of 2°C.

Exotic alleles contribute to heat tolerance in wheat under field conditions.

Global warming poses a major threat to food security and necessitates the development of crop varieties that are resilient to future climatic instability. By evaluating 149 spring wheat lines in the field under yield potential and heat stressed conditions, we demonstrate how strategic integration of exotic material significantly increases yield under heat stress compared to elite lines, with no significant yield penalty under favourable conditions. Genetic analyses reveal three exotic-derived genetic loci underlying this heat tolerance which together increase yield by over 50% and reduce canopy temperature by approximately 2 °C.

Awned versus awnless wheat spikes: does it matter?

Awnless and awned wheat is found across the globe. Archeological and historical records show that the wheat spike was predominantly awned across the many millennia following domestication. Thus, ancient farmers did not select against awns at least until the last millennium.

Prediction of Photosynthetic, Biophysical, and Biochemical Traits in Wheat Canopies to Reduce the Phenotyping Bottleneck.

To achieve food security, it is necessary to increase crop radiation use efficiency (RUE) and yield through the enhancement of canopy photosynthesis to increase the availability of assimilates for the grain, but its study in the field is constrained by low throughput and the lack of integrative measurements at canopy level. In this study, partial least squares regression (PLSR) was used with high-throughput phenotyping (HTP) data in spring wheat to build predictive models of photosynthetic, biophysical, and biochemical traits for the top, middle, and bottom layers of wheat canopies. The combined layer model predictions performed better than individual layer predictions with a significance as follows for photosynthesis = 0.

Phenotypic variation in photosynthetic traits in wheat grown under field versus glasshouse conditions.

Recognition of the untapped potential of photosynthesis to improve crop yields has spurred research to identify targets for breeding. The CO2-fixing enzyme Rubisco is characterized by a number of inefficiencies, and frequently limits carbon assimilation at the top of the canopy, representing a clear target for wheat improvement. Two bread wheat lines with similar genetic backgrounds and contrasting in vivo maximum carboxylation activity of Rubisco per unit leaf nitrogen (Vc,max,25/Narea) determined using high-throughput phenotyping methods were selected for detailed study from a panel of 80 spring wheat lines.

Effect of Flowering Time-Related Genes on Biomass, Harvest Index, and Grain Yield in CIMMYT Elite Spring Bread Wheat.

Grain yield (YLD) is a function of the total biomass (BM) and of partitioning the biomass by grains, i.e., the harvest index (HI).

Nocturnal stomatal conductance in wheat is growth-stage specific and shows genotypic variation.

Nocturnal stomatal conductance (g ) represents a significant source of water loss, with implications for metabolism, thermal regulation and water-use efficiency. With increasing nocturnal temperatures due to climate change, it is vital to identify and understand variation in the magnitude and responses of g in major crops. We assessed interspecific variation in g and daytime stomatal conductance (g ) in a wild relative and modern spring wheat genotype.

Field-based remote sensing models predict radiation use efficiency in wheat.

Wheat yields are stagnating or declining in many regions, requiring efforts to improve the light conversion efficiency, known as radiation use efficiency (RUE). RUE is a key trait in plant physiology because it links light capture and primary metabolism with biomass accumulation and yield, but its measurement is time consuming and this has limited its use in fundamental research and large-scale physiological breeding. In this study, high-throughput plant phenotyping (HTPP) approaches were used among a population of field-grown wheat with variation in RUE and photosynthetic traits to build predictive models of RUE, biomass, and intercepted photosynthetically active radiation (IPAR).

High Throughput Field Phenotyping for Plant Height Using UAV-Based RGB Imagery in Wheat Breeding Lines: Feasibility and Validation.

Plant height (PH) is an essential trait in the screening of most crops. While in crops such as wheat, medium stature helps reduce lodging, tall plants are preferred to increase total above-ground biomass. PH is an easy trait to measure manually, although it can be labor-intense depending on the number of plots.

Uncovering candidate genes involved in photosynthetic capacity using unexplored genetic variation in Spring Wheat.

To feed an ever-increasing population we must leverage advances in genomics and phenotyping to harness the variation in wheat breeding populations for traits like photosynthetic capacity which remains unoptimized. Here we survey a diverse set of wheat germplasm containing elite, introgression and synthetic derivative lines uncovering previously uncharacterized variation. We demonstrate how strategic integration of exotic material alleviates the D genome genetic bottleneck in wheat, increasing SNP rate by 62% largely due to Ae.

Overcoming the trade-off between grain weight and number in wheat by the ectopic expression of expansin in developing seeds leads to increased yield potential.

Wheat is the most widely grown crop globally, providing 20% of all human calories and protein. Achieving step changes in genetic yield potential is crucial to ensure food security, but efforts are thwarted by an apparent trade-off between grain size and number. Expansins are proteins that play important roles in plant growth by enhancing stress relaxation in the cell wall, which constrains cell expansion.

Breeder friendly phenotyping.

The word phenotyping can nowadays invoke visions of a drone or phenocart moving swiftly across research plots collecting high-resolution data sets on a wide array of traits. This has been made possible by recent advances in sensor technology and data processing. Nonetheless, more comprehensive often destructive phenotyping still has much to offer in breeding as well as research.

New avenues for increasing yield and stability in C3 cereals: exploring ear photosynthesis.

Small grain cereals such as wheat, rice and barley are among the most important crops worldwide. Any attempt to increase crop productivity and stability through breeding implies developing new strategies for plant phenotyping, including defining ideotype attributes for selection. Recently, the role of non-foliar photosynthetic organs, particularly the inflorescences, has received increasing attention.

Genetic variation for photosynthetic capacity and efficiency in spring wheat.

One way to increase yield potential in wheat is screening for natural variation in photosynthesis. This study uses measured and modelled physiological parameters to explore genotypic diversity in photosynthetic capacity (Pc, Rubisco carboxylation capacity per unit leaf area at 25 °C) and efficiency (Peff, Pc per unit of leaf nitrogen) in wheat in relation to fertilizer, plant stage, and environment. Four experiments (Aus1, Aus2, Aus3, and Mex1) were carried out with diverse wheat collections to investigate genetic variation for Rubisco capacity (Vcmax25), electron transport rate (J), CO2 assimilation rate, stomatal conductance, and complementary plant functional traits: leaf nitrogen, leaf dry mass per unit area, and SPAD.

Yielding to the image: How phenotyping reproductive growth can assist crop improvement and production.

Reproductive organs are the main reason we grow and harvest most plant species as crops, yet they receive less attention from phenotyping due to their complexity and inaccessibility for analysis. This review highlights recent progress towards the quantitative high-throughput phenotyping of reproductive development, focusing on three impactful areas that are pivotal for plant breeding and crop production. First, we look at phenotyping phenology, summarizing the indirect and direct approaches that are available.

Elucidating the genetic basis of biomass accumulation and radiation use efficiency in spring wheat and its role in yield potential.

One of the major challenges for plant scientists is increasing wheat (Triticum aestivum) yield potential (YP). A significant bottleneck for increasing YP is achieving increased biomass through optimization of radiation use efficiency (RUE) along the crop cycle. Exotic material such as landraces and synthetic wheat has been incorporated into breeding programmes in an attempt to alleviate this; however, their contribution to YP is still unclear.

Hyperspectral reflectance as a tool to measure biochemical and physiological traits in wheat.

Improving photosynthesis to raise wheat yield potential has emerged as a major target for wheat physiologists. Photosynthesis-related traits, such as nitrogen per unit leaf area (Narea) and leaf dry mass per area (LMA), require laborious, destructive, laboratory-based methods, while physiological traits underpinning photosynthetic capacity, such as maximum Rubisco activity normalized to 25 °C (Vcmax25) and electron transport rate (J), require time-consuming gas exchange measurements. The aim of this study was to assess whether hyperspectral reflectance (350-2500 nm) can be used to rapidly estimate these traits on intact wheat leaves.

Variation in developmental patterns among elite wheat lines and relationships with yield, yield components and spike fertility.

Developmental patterns strongly influence spike fertility and grain number, which are primarily determined during the stem elongation period (i.e. time between terminal spikelet phase and anthesis).