Single-stage posterior resection of the transversal process combined with an intervertebral foraminal approach for debridement, interbody fusion, internal fixation for the treatment of lumbar tuberculosis and psoas major abscess.

Purposes: To investigate the feasibility and clinical efficacy of the treatment for lumbar tuberculosis with psoas major abscess with single-stage posterior resection of the transversal process combined with an intervertebral foraminal approach for debridement, interbody fusion, internal fixation.

Methods: This retrospective study evaluated the clinical data of 24 patients (14 males and 10 females) with lumbar tuberculosis and psoas major abscess admitted to the Comprehensive Surgery from June 2016 to June 2019. All patients were treated with the single-stage posterior approach to remove the transverse process combined with the intervertebral foramina approach for debridement, interbody fusion, internal fixation.

Mycobacterium culturing and drug resistance of osteoarticular tuberculosis in Xinjiang, China.

This study aims to elucidate the strains and drug resistance of mycobacterium isolated from osteoarticular tuberculosis (OATB) patients and provide a reference for the diagnosis and treatment of OATB.Sixty-nine clinically diagnosed and surgically treated OATB patients were collected in time period of January 2017 to December 2018 at the First Affiliated Hospital of Xinjiang Medical University. The BACTEC MGIT 960 system was used for mycobacteria culturing, strain identification, and drug susceptibility testing, and the mycobacteria culture positive rate, species distribution, and drug resistance were analyzed.

Synovial Fluid HMGB-1 Levels are Associated with Osteoarthritis Severity.

Background: Recent data have shown that enhanced cytokine production in knee osteoarthritis (OA) synovium are believed to promote pathological OA. High-mobility group box 1 (HMGB-1) as a well-known pro-inflammatory cytokine may influence the development of knee OA. The purpose of this study is to analyze the amount of and location of HMGB-1 in OA synovium and to compare it with controls who are afflicted with acute meniscal or cruciate ligament tears.

Research of osteoblastic induced rat bone marrow mesenchymal stem cells cultured on β-TCP/PLLA porous scaffold.

Background: Ceramic and polymer composite scaffolds are widely used in tissue engineering for bone tissue regeneration. Composite of β-tricalcium phosphate (β-TCP) and poly L-lactic acid (PLLA), due to its biocompatibility and biodegradability, is widely used in bioengineering. However, optimal ratio, porosity and pore size of this kind of scaffolds were not very clear yet.

In vitro evaluation of rhBMP-2-induced expression of VEGF in human adipose-derived stromal cells.

Bone Morphogenetic Protein 2 (BMP-2) plays a key role in skeletal development, repair and regeneration. Our previous studies indicate that recombinant human BMP-2 (rhBMP-2) can stimulate osteogenic differentiation and promote angiogenesis through the up-regulation of Vascular Endothelial Growth Factor (VEGF), while the underlying mechanism of the BMP-2 effect on human cells is not well understood. To gain a better understanding of BMP-2-induced angiogenesis, we further characterized the effect of rhBMP-2 on VEGF expression in human adipose-derived stromal cells (hASCs) by RT-PCR and ELISA.

Enhanced osteogenic activity of mesenchymal stem cells and co-modified BMP-2 and bFGF genes.

Background: We co-modified rat mesenchymal stem cells (rMSCs) with bone morphogenetic protein-2 (BMP-2) and basic fibroblast growth factor (bFGF) genes, and then evaluated their osteogenic activity in vitro and evaluated ectopic bone formation in vivo.

Material/methods: Nude mice (male, 20±2 g) were randomly divided into 2 groups: a control group (n=30) and a transfection group (n=30). In the control group, rMSCs+scaffold was implanted into the intramuscular pocket in the left femur, and BMP-2 gene modified rMSCs+scaffold was implanted into the intramuscular pocket in the right femur.

[Concentration- or time-dependent manner of recombinant bone morphogenetic protein 2 in regulating expression of vascular endothelial growth factor].

Objective: To study biological rule of recombinant human bone morphogenetic protein 2 (rhBMP-2) in regulating the expression of vascular endothelial growth factor (VEGF) of adipose-derived stem cells (ADSCs) at different induced concentrations and time points at gene level and protein level.

Methods: ADSCs were separated from adult human adipose tissues and cultured until passage 3. After ADSCs were induced by rhBMP-2 in concentrations of 0, 50, 100, and 200 ng/mL respectively for 24 hours, and by 100 ng/mL rhBMP-2 for 3, 6, 12, 18, 24, 36, and 48 hours (ADSCs were not induced at corresponding time point as controls) respectively, the VEGF mRNA and protein expressions were detected by RT-PCR and ELISA.

[Clinical characteristics and treatment for patients with low back and leg pain caused by lumbar annular tears].

Objective: To investigate whether annular tears is a cause of low back and radiating leg pain and explore the clinical characteristics and treatment for patients with this condition.

Methods: A total of 34 patients with low back and radiating leg pain, but without lumbar disc herniation on CT (computed tomography) or MRI (magnetic resonance imaging), were examined by electrophysiological studies and discography to identify whether there were or not annular tears and nerve root injury and decipher the relations between them. The series included 15 males and 19 females with an average age of 45.

[Intraobserver and interobserver reliability of the King, Lenke, and PUMC classification systems for idiopathic scoliosis].

Objective: To evaluate the intraobserver and interobserver reliability and main influencing factors of the King, Lenke, and PUMC classification systems for idiopathic scoliosis (IS) using radiographs that had not been premeasured.

Methods: Fifty-six patients with IS, 9 male and 47 female, aged 15.4 (11 - 18), underwent preoperative X-ray photography of spine standing full-length posteroanterior and lateral films and left and right supine side-bending radiographs.

Treatment of tibial defect and bone nonunion with limb shortening with external fixator and reconstituted bone xenograft.

Objective: To explore the effect of external fixator and reconstituted bone xenograft (RBX) in the treatment of tibial bone defect, tibial bone nonunion and congenital pseudarthrosis of the tibia with limb shortening.

Methods: Twenty patients (13 males and 7 females) with tibial bone defect, tibial bone nonunion or congenital pseudarthrosis of the tibia with limb shortening were treated with external fixation. Two kinds of external fixators were used: a half ring sulcated external fixator used in 13 patients and a combined external fixator in 7 patients.