Molecular Characterization of Strain Isolated from Yellow Mealworms, , in The Netherlands.

Insect culture has developed rapidly worldwide; it faces important security and safety control issues, including animal infections and disease development. In the Netherlands, in 2021, a ~30% mortality of mealworms, , occurred at one farm, where over-humid sites in the substrate were observed. Bacterial cultures from both the external and internal partsof fry and larger mealworms were identified by MALDI-TOF to predominantly and .

Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods.

This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24-28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents.

Fast and accurate identification by MALDI-TOF of the zoonotic serovar E of Vibrio vulnificus linked to eel culture.

Vibrio vulnificus is a zoonotic pathogen that can cause death by septicaemia in farmed fish (mainly eels) and humans. The zoonotic strains that have been isolated from diseased eels and humans after eel handling belong to clade E (or serovar E (SerE)), a clonal complex within the pathovar (pv.) piscis.

The Warramiri website: applying an alternative Yolŋu epistemology to digital development.

The intergenerational transmission of traditional language and culture is at the core of Yolŋu Indigenous knowledge practices. The homeland of Gäwa in remote Arnhem Land, Northern Territory, was established by Warramiri clan kinship networks to provide an appropriate place for this crucial role to continue. Technologies have long played a part in this transmission process, but can databases, websites and other digital storage mediums harmonise with existing Yolŋu epistemological and ontological frameworks? In considering an alternative approach to digital development, we rely on the Yolŋu elements of performative epistemology, multiple perspectives and a fundamental, narrative base.

Q fever in pregnant goats: humoral and cellular immune responses.

Q fever is a zoonosis caused by the intracellular bacterium Coxiella burnetii. Both humoral and cellular immunity are important in the host defence against intracellular bacteria. Little is known about the immune response to C.

Q fever in pregnant goats: pathogenesis and excretion of Coxiella burnetii.

Coxiella burnetii is an intracellular bacterial pathogen that causes Q fever. Infected pregnant goats are a major source of human infection. However, the tissue dissemination and excretion pathway of the pathogen in goats are still poorly understood.

Daily suppression of discrete emotions during the work of police service workers and criminal investigation officers.

The aim of the present research among Dutch police officers was to examine whether fluctuations in emotional job demands predict exhaustion through the suppression of discrete emotions. A first diary study (N =25) tested how the suppression of discrete emotions is related to exhaustion at the end of the work shift of police call-center service workers. Results revealed that suppressing anger was positively related to exhaustion at the end of a work shift, whereas suppressing happiness was not.

Influence of HLA-A, HLA-B, and HLA-DR matching on rejection of random corneal grafts using corneal tissue for retrospective DNA HLA typing.

Aim: To establish if coincidental HLA-A, HLA-B, and HLA-DR tissue matching is associated with a reduced likelihood of corneal graft rejection.

Methods: Organ culture preserved random donor corneas were used for penetrating keratoplasty (PKP). Corneal tissue from all graft recipients and donors or blood samples from recipients after repeated transplantation were obtained in order to perform retrospective molecular HLA typing.

Detection of herpes simplex virus type 1, 2 and varicella zoster virus DNA in recipient corneal buttons.

Aim: To study the value of polymerase chain reaction (PCR) analysis, to detect viral DNA in recipient corneal buttons taken at the time of penetrating keratoplasty (PKP) in patients with an initial diagnosis of herpetic stromal keratitis (HSK). Since HSK has a tendency to recur, an accurate diagnosis of previous HSK could be the reason to start antiviral treatment immediately, thereby possibly decreasing the number of graft failures due to recurrent herpetic keratitis.

Methods: Recipient corneal buttons and aqueous humour (AH) samples were obtained at the time of PKP from HSK patients (n=31) and from other patients (n=78).

Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection.

Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis.

Molecular cloning of a new angiopoietinlike factor from the human cornea.

Purpose: To isolate tissue-specific gene products that contribute to corneal integrity.

Methods: A cDNA library was constructed and differentially hybridized. Cornea-specific clones were purified and further characterized.