Exogenous allogenic fragmented double-stranded DNA is internalized into human dendritic cells and enhances their allostimulatory activity.

Exogenous allogenic DNA as nucleosome-free fragments reaches main cellular compartments (cytoplasm, nucleus) of human dendritic cells and deposits in the nuclear interchromosomal space without visibly changing in linear size. The presence of such allogenic fragmented DNA in medium in which human dendritic cells are cultured produces an enhancement of their allostimulatory activity. This enhancement is comparable to that produced by the standard maturation stimulus lipopolysaccharide Escherichia coli.

[Functional asymmetry of the brain hemispheres: the role in pathogenesis of ectromelia and melanoma in (CBAxC57BL/6)F1 mice].

The object of the research was to identify role of functional nervous asymmetry in formation of pathology in experimental animals. The role of functional asymmetry of the brain hemispheres in development of ectromelia and melanoma at (CBAxC57BL/6)F1 mice was investigated. It has been established, that mice with domination of motor functions in the left hemisphere ("right-handed") are more resistant against infection with ectromelia virus, and melanoma B-16 grows more slowly in these mice as compated with animals with domination of motor functions of the right hemisphere ("left-handed").

[Leukocyte stimulation by DNA fragments shored up protamine in cyclophosphamide-induced leukopoiesis in mice].

Our study showed that protamine (80% w/w to DNA) effectively protected its molecules from degradation by native nucleases of the mammalian blood serum. Exogenous DNA bound to protamine effectively stimulated restoration of cyclophosphamide-induced leukopoiesis in mice. It is suggested that the phenomenon was due to repair processes taking place in hemopoietic stem cells damaged by a cross-linking cytostatic drug such as cyclophosphamide.

[Frequency of the HLA-DP genes and the antigens of the HLA-A, -B, -Cw, and -DR loci in Tuvinians].

The results of DNA typing of the HLA-DPB1 gene among Tuvinians are described. The allele 0201 was found to be the most frequent (26.66%); then, in order of decreasing frequency, followed the alleles 0401 (26.

[Quantitative analysis of the phospholipid composition of the myocardium in experimental diabetes mellitus: effect of total ischemia].

Phospholipid composition was studied in the myocardium of rats and rabbits with alloxan diabetes developed after intravenous of intraperitoneal administration of alloxan and complicated by 60-min total ischemia. The diabetes developed within 4 weeks thereafter heart tissue was isolated and used for biochemical analyses. Total cardioplegic ischemia was simulated in the perfused heart after drastic interruption of perfusion.

[Frequency distribution of HLA phenotypes, haplotypes and alleles in ethnic groups of Taĭmyr and Chukotka].

HLA typing data of two little populations living in Tajmyr peninsula (Dolgans and Nganasans) and Chukotka peninsula (Chukchas and Chuvantses) were presented. Our data were compared with the International standard distribution of class I HLA antigens in orients and its distribution in orients who live in the Asian part of Russia. Indexes of genetic distribution and genetic likeness were calculated.

[Changes in central hemodynamics and microcirculation during laser therapy in patients with coronary insufficiency].

The study was undertaken to examine 45 patients with Stages IIB-III heart failure (HF) by the classification developed by V. Kh. Vasilenko and N.

[Energy metabolism and contractile function of the heart in diabetic cardiomyopathy: effect of ischemia and reperfusion].

Physiological parameters, rates of mitochondrial respiration, high energy phosphate levels and creatine phosphokinase (CPK) activity were investigated in the hearts from control and alloxan-induced diabetic rabbits before and after 40-min total ischemia and reperfusion. Diabetic hearts demonstrated significant decreases in the rates of contraction (+dP/dt) and relaxation (-dP/dt), heart rates and cardiac work compared to control hearts. Determination of mitochondrial respiration rates in saponin-skinned fibers showed a low mitochondrial respiratory function in diabetic hearts.

[The use of helium-neon laser in drug-resistant cardiac arrhythmias].

Out of 85 patients with cardiac arrhythmias in the presence of chronic coronary heart disease, 28 who were resistant to ethacisine and allapinine were included into the study. They had frequent and persistent arrhythmias. The patients were divided into 2 groups: (1) the patients receiving intravenous He-Ne laser therapy in combination with one of the above drugs; (n = 17) and (2) those taking He-Ne laser therapy alone (n = 11).

[Comparative analysis of indicators of the immune status of population of the Eastern regions of the USSR].

Altogether 1,500 healthy residents of seven cities situated in the Asian part of the USSR were examined. In Novosibirsk, Tomsk, Tyumen, Norilsk, Magadan, Yakutsk and Ussuriisk, the people were examined for the blood levels of T and B lymphocytes, the ratio of regulatory subclasses of T lymphocytes, the concentration of IgG, IgM and IgA, and for the content of immune complexes. Analysis was made of the general and regional regularities in changes seen in the immune system depending on climatic and geographic factors.

[Disorders of cardiac rhythm and the changes in Q-T interval in mitral valve prolapse syndrome].

To study the incidence and nature of cardiac arrhythmias and the changes in the corrected Q-T interval, 60 patients with the prolapsed mitral valve syndrome were examined. All the patients underwent resting ECG and leg isometric exercise. 24-hour ECG monitoring was performed in 48 patients.

[Use of isometric exercise to enhance the diagnostic value of dipyridamole testing in patients with angina pectoris].

A dipyridamole test was performed in 40 patients with coronary heart disease who were divided into 2 groups, each consisting of 20 subjects. The dipyridamole test alone was performed in the patients from Group 1, while the agent was given by using manual dynamometry and increasing venous return in those from Group 2. Positive results were obtained in 55 and 85%, respectively (p less than 0.

[Functional state of the left regions of the heart in healthy persons during isometric exercise].

Hemodynamic parameters of left and atrial contractile and pump functions were examined in 25 healthy males undergoing leg isometric exercise, by employing echocardiography. Analysis of changes in intracardiac hemodynamics showed that for provision of a marked pressor reaction occurring in healthy persons during isometric exercise, there was enhanced left ventricular myocardial contractility and thereby higher stroke and minute volumes. To maintain the required increase in stroke volume at the maximal exercise level, an accessory mechanism of compensation gears (by the Frank-Starling type), which operates due to myocardial diastolic relaxation, elevated venous blood supply at the expense of sucking cardiac action, and augmented left atrial contractility.

[Evaluation of the effect of intravenous administration of streptodecase on the extent of the damage in myocardial infarction].

Precordial mapping was employed to examine 29 patients with acute anterior transmural myocardial infarction (MI). Seventeen of them received intravenous streptokinase, eleven underwent symptomatic therapy. The size of the lesion was assessed in the course of the disease.

[Changes in fluid spaces in patients with heart failure under the effects of isolated ultrafiltration].

The study was undertaken to examine 22 patients with chronic heart failure of Stages IIB-III according to the classification developed by V. Kh. Vasilenko and N.

[Use of isometric load for evaluation of the anti-arrhythmia effect of drugs].

Possible use of pedal isometric stress for the assessment of antiarrhythmic effect of ethacizine was explored in 34 patients with various heart rhythm disorders. They showed similar results of pedal isometric stress test and 24-hour ECG monitoring. Heart rhythm monitoring in the presence of the acute test and a treatment course demonstrated complete agreement between positive results of the pedal isometric stress test and 24-hour ECG monitoring in 85% of the patients, exhibiting an obvious antiarrhythmic effect.

[Use of a static exercise test for evaluating anti-angina effects of drugs in patients with ischemic heart disease at a polyclinic].

Only in 115 of 320 patients who had attended the polyclinic for cardialgias, static exercise and bicycle ergometer tests could be performed because of medical contraindications and the patients' refusal to participate in the investigation. Positive results were found in 15 patients who had undergone bicycle ergometer tests and in 12 patients who did static exercise. It has been shown that static exercise tests may be useful for selecting antianginal drugs (glyceryl trinitrate, anapriline, corinfar) for outpatients with coronary heart disease on an individual basis.

[Functional status of the left side of the heart in patients with ischemic heart disease during isometric exercise].

Hemodynamic parameters of left-ventricular and left-atrial contractility and pumping function were examined in 47 males with postinfarction cardiosclerosis and no clinical signs of circulatory insufficiency and in 30 normal male subjects, exposed to isometric leg exercise with the highest possible compression force. At the height of exercise, normal subjects showed increased left-ventricular myocardial contractility along with the initiation of the Frank-Starling mechanism, spreading to the left atrium as well, while coronary patients responded to increased stress by growing left-atrial reservoir function during the first minutes of exercise, and activated left-atrial transport and pumping functions afterwards. Isometric leg exercise is capable of detecting left-atrial and left-ventricular pumping and contractility disorders in coronary patients.

[Comparative evaluation of the results of the bicycle ergometry test in patients with stress stenocardia after treatment with propranolol and nifedipine].

The results of bicycle ergometry (BE) following administration of placebo, 80 mg propranolol (anaprilin), 30 mg nifedipine (corinfar) and a combination of 60 mg propranolol and 20 mg nifedipine were reviewed in coronary patients with angina pectoris of functional classes (FC) II-IV. Patients with FC-II angina showed the greatest increment in stress tolerance after nifedipine administration, and patients with FC-IV angina, after propranolol, apparently due to a predominance of the functional (coronaro-spastic) pathogenetic factor in the FC-II angina, and the organic factor, in angina of more severe functional classes. In patients belonging to FC-III, the two drugs had similar antianginal efficiency.