[Use of serotonin for treatment of patients with diabetic angiopathies].

In 26 patients (female 11, males--15) with diabetic angiopathy (necrosis of the foot or toes) local measurement of pO2 by transcutaneous mode was carried out before and during introduction of serotonine adipinate. Simultaneously with evaluation of pO2 in 14 patients electromyography (EMG) was made. Serotonine adipinate was introduced intravenously with a speed of 5.

[Neurologic disorders in patients with Madelung's lipomatosis].

The authors had 21 patients with a rare disease, Madelung's lipomatosis. Vegetative disorders of segmental and suprasegmental levels were revealed in all patients. The character of neuropathies in Madelung's lipomatosis is discussed in detail.

[Changes in the human brain and spinal cord after short-term cardiac arrest and prolonged respiratory resuscitation].

Clinicomorphological data on 12 cases of long-term APV (up to 35 days) are reported. The degree of morphological changes was found to vary with CNS compartment. The authors emphasize clinicomorphological alterations in the spinal cord, particularly in the cervical segments, where almost complete absence of cells and reactivity of glial elements were observed.

[An electron radioautographic study of the dynamic decrease in the level of RNA synthesis in human brain cells at different times after death].

The above study is performed in neurons, oligodendrocytes and capillary endotheliocytes of human brain 4-13 hours after death. Brain biopsies after the surgery and early necropsies served as the material. RNA synthesis in some neurons and glial cells is retained to some degree 8-13 hours after death.

[Amplification of DNA for 15-30 minutes in single-use pipette tips].

Amplification of 200-1000-bp DNA fragments was performed in 15-30 min using a rapid thermal cycler based on the commercial instrument TC-1000-1 (IRLEN, St. Petersburg, Russia). Plastic pipette tips were used as thin walled, high surface to volume-ratio tubes, to increase the rate of heating (cooling) of 20 microliters samples, which allowed the time required for DNA amplification to be considerably reduced (5-10 times).

[Analysis of serum antibodies to nerve tissue antigens in patients with lateral amyotrophic sclerosis].

We used non-direct immunofluorescence microscopy, immunoblotting and affinity chromatography on A-protein Superose to study antibodies to neural tissue antigens in sera from 11 patients with ALS and from 10 healthy donors. In all sera the majoric antigens had molecular masses of 150-200kD, 70kD and 50kD. No consistent differences were found between ALS patients and controls.

[The clinico-epidemiological characteristics of burn trauma].

Analysis of medical and sociopsychological factors in 231 burn victims was provided during 4-year randomized study. Groups of burn-prone (working class males, alcohol abusers, burned drunk, violating safety measures, with concomitant somatic and brain disorders and maladjustment) and non-prone individuals were elucidated. Shifts in Spielberger's state and trait anxiety scores depend on the damage of their somatic and nervous "underground".

[Surgical treatment of patients with chronic calculous pancreatitis].

The article discusses experience in the examination of 109 patients with chronic calculous pancreatitis (CCP), 102 of them were treated by operation (a total of 130 operations were carried out). Calculous cholecystitis was a consequence of alcohol intake in 102 cases, and was due to other causes in 7 cases. In 71% of cases CCP was attended by the development of indirect signs of pancreatic hypertension and in 45.

[Nervous and mental disorders in patients with chronic alcoholic pancreatitis].

Presented are the results of comprehensive CNS examinations in 53 alcoholics with chronic pancreatitis. Related psychopathological and neurological syndromes are specified. Hepatopancreatoduodenal and CNS abnormalities were found directly correlated.

[Psychotropic therapy during anesthesia in the treatment of burn patients at various stages of the disease].

The efficacy of various types of psychotropic therapy was comparatively assessed in patients with burns and borderline neuro-psychic disorders. The efficacy of therapy was determined using standard uniform scales for the evaluation of the effect of psychotropic agents and by analysis of statistical indexes of the heart rhythm and respiratory pattern. Psychotropic therapy significantly reduced psychopathological symptoms and a more marked normalization of autonomous body functions was observed.

[45 kDa fragment of the kinesin molecule possesses high ATPase activity and binds to microtubules].

Kinesin is a mechano-chemical ATPase capable to move particles along microtubules and microtubules along the solid substrate. Molecule of bovine brain kinesin is a heterotetrameric unit consisting of two heavy (120 kDa) and two light (62 kDa) chains. We used limited proteolysis to study the location of the functional sites on the kinesin molecule.

[Clinical variants of neurosis-like disorders in burns].

A total of 130 patients were treated in a burn unit of a hospital. Three stages of neurosis-like disorders were noted: (1), acute stress reaction close in its structure to prodromal phase of exogenous psychoses and approximately coinciding with the burn shock; (2), formation and dissolution of psychopathologic syndromes (asthenic, depressive and asthenic-depressive), resembling the so-called "transitional" syndromes coinciding with toxemias and septic pyemias; (3), late psychogenesis (mobilization, pseudoeuphoric and hypochondriac).

[Organization of stress fibrils in cultured fibroblasts studied by using an actin filament-fragmenting protein].

Earlier we isolated a 1:1 complex of 90 kD-protein and actin from bovine brain. This complex was able to fragment actin filaments. Effects of this complex on the cytoskeleton of mouse and quail embryo fibroblasts are described.

[Study of a protein complex from the brain which reduces actin viscosity. II. The complex inhibits elongation of actin filaments at the end preferable for polymerization and fragments the filaments].

Functional properties of the protein complex from bovine brain that shortens actin filaments are described. In the presence of Ca2+ complex shortens actin filaments and increases the initial rate of actin polymerization. In the absence of free calcium ions the complex loses its accelerating effect on actin polymerization, but still possesses actin filament shortening activity.

[Study of a protein complex from the brain which reduces actin viscosity. I. Composition and various properties of the complex].

The method of isolation from bovine brain of a preparation containing 90 kDa- and 42 kDa-proteins is described. This preparation shortens actin filaments and therefore decreases viscosity of F-actin. The 42 kDa-component was identified as actin by one-dimensional peptide mapping.

[Glycerol models of the ciliated epithelium of bronchial mucosa and their use in the diagnosis of chronic nonspecific lung diseases].

The method of extraction of ciliated epithelium from biopsy samples of human bronchial mucosa with glycerol is suggested. Permeabilized cilia of glycerol-extracted cells can be easily reactivated by exogenous ATP. This method was used for the study of ciliary dyskinesia in patients with chronic lung diseases.