TP53 overexpression in recurrent endometrial carcinoma.

Objective: To study alterations within the p53 pathway in relation to the development of recurrent stage I endometrioid endometrial carcinoma.

Methods: Paraffin-embedded tumor tissue of both primary and recurrent tumors from 44 patients with and 44 without recurrence was used for immunohistochemical analysis of TP53, hMdm2, P21(Waf1/Cip1) and M30. DNA was extracted, and mutation analysis of p53 (exon 5-8, 11) was performed by direct sequencing.