Absence of direct relationship between intraperitoneal cellular influx and resistance to experimental peritonitis.

Intraperitoneal inflammation is an essential defence mechanism against microbial invasion of the abdominal cavity. We have recently demonstrated that a single contact with heat killed E. Coli or Staphylococcus aureus increased the intraabdominal leukocyte influx in rats later challenged by these microorganisms.

Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies.

Anti-CD3 antibodies induce a quick and profound depletion of peripheral blood mononuclear cells (PBMCs) that is not well understood. We studied the effect of OKT3, a mouse monoclonal antibody against the human CD3 complex, on the in vitro adhesion of human PBMCs to monolayers of fresh and fixed human umbilical vein endothelial cells (HUVECs). OKT3 induced an increased adhesiveness of PBMCs.

Modulation of inflammatory reactions by previous contact with Escherichia coli in rats.

Objective: To find out if contact with Escherichia coli had any effect on a subsequent inflammatory reaction induced by the same micro-organism.

Design: Controlled laboratory study.

Setting: University laboratory.

Modulation of inflammatory reactions by surgical trauma: lack of relationship with corticosteroid secretion.

The effect of surgery on inflammation was studied in male Wistar R/A rats using the carrageenin-induced edema model. Swelling of the paw was measured in standardized arbitrary units 2, 4, and 6 hr after a subcutaneous injection of carrageenin iota in the subplantar region of the right hind limb. It was significantly depressed in rats submitted to laparotomy (5.

Effect of surgical trauma on various aspects of the immune response in rats.

The effects of a standard laparotomy on different aspects of the immune response were studied in Wistar R/A rats. When the antigen was administered at the time of operation, antibody synthesis against sheep red blood cells (SRBC), a thymo-dependent antigen was significantly stimulated. The same phenomenon was observed when a soluble antigen extracted from SRBC was injected during the surgical procedure.

Effect of surgical trauma on delayed type hypersensitivity.

There is no general agreement in the literature concerning the effects of surgery on delayed type hypersensitivity (DTH). Therefore, the effect of a standard laparotomy on DTH was studied in rats and measured by the ear index method of Kostiala. Surgery depresses significantly the ear index in animals challenged at the time of operation (2.

Effect of surgical procedures on the humoral responsiveness of rats.

The humoral response after an intravenous injection of 10(9) sheep red blood cells was studied in a control group of rats and in rats submitted to three types of surgical procedures. The operated upon rats had an increased antibody synthesis, and this enhancing effect seemed to be proportional to the intensity of surgical trauma. The stimulatory effect of operation was also observed when the antigen was administered in the operative field.

Kinetics of humoral responsiveness and antigenic distribution in operated rats.

Wistar R/A rats were injected intravenously with 10(9) sheep red blood cells (SRBC) prior to, during or after a standard laparotomy. Stimulation of anti-SRBC antibody synthesis was already observed when the antigen was given 4 h prior to surgery and was maximal if SRBC were administered at the time of operation. The enhancing effect on the immune response lasted for 2 days after surgery.

Stimulation of antibody synthesis induced by surgical trauma in rats.

The effect of a standard laparatomy on antibody synthesis was studied in Wistar R/A rats recieving an intravenous injection of 10(9) sheep red blood cells (SRBC) during the surgical procedure. Anti-SRBC antibody titres were significantly higher in operated animals than in controls. When SRBC were given 2 hr after the surgical procedure, stimulation of antibody synthesis still persisted, but when the antigen was administered 24 hr after laparotomy, no significant difference could be detected between the operated animals and controls.

[Presence of soluble red cell antigens in antilymphocyte serum or in serum of normal rabbits absorbed with sheep red cells (author's transl)].

The treatment of Wistar rats with normal rabbit sera or rabbit anti-Rat lymphocyte sera preabsorbed with sheep red blood cells induces variable degrees of immunisation against these erythrocytes. This phenomenon is due to transfer of soluble erythrocyte antigen released during the absorption procedure.

Organ distribution of sheep red blood cells in ALS-treated rats.

The inter-organ distribution of radioactivity in rats injected with 51Cr-labelled SRBC is altered after treatment with ALS absorbed with this antigen. The alteration is due to the presence of soluble SRBC antigens in the serum and subsequent immunization of the tested animals. The 51Cr distribution does not correspond to the uptake of antigenic material in immunized rats.