sp. nov. and sp. nov., isolated from surface seawater of the Pacific Ocean.

Two Gram-negative, non-spore-forming, non-motile, non-flagellated bacteria, designated strains D6 and DH64, were isolated from surface water of the Pacific Ocean. For strain D6, growth occurred at 10-40 °C, pH 5.5-9.

sp. nov. and sp. nov., two novel members isolated from deep-sea environments.

Two Gram-stain-negative, rod-shaped and non-motile strains, designated as DY56-A-20 and G39, were isolated from deep-sea sediment of the Pacific Ocean and deep-sea seawater of the Indian Ocean, respectively. Strain DY56-A-20 was found to grow at 15-37 °C (optimum, 28 °C), at pH 6.0-10.

sp. nov., a marine bacterium isolated from deep seawater.

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated C281, was isolated from seawater sampled at the Marshallese seamount chain. Results of 16S rRNA gene analysis revealed that strain C281 was most closely related to CZ-AZ5 with 92.7 % sequence similarity.

Aestuariibaculum sediminum sp. nov., a marine bacterium isolated from a tidal flat in Zhoushan.

A Gram-staining-negative, non-motile, strictly aerobic bacterium, designated as strain TT11, was isolated from a sediment sample of a tidal flat connected in Zhoushan, China. Cells of strain TT11 are spherical, halotolerant, catalase- and oxidase-positive, and produce carotenoid-like pigments. Colonies were 0.

The Complete Genome of Emcibacter congregatus ZYL, a Marine Bacterium Encoding a CRISPR-Cas 9 Immune System.

Emcibacter congregatus ZYL was isolated from a sediment sample cultured in situ in a coast located in the East China Sea. The genome of E. congregatus ZYL was sequenced and assembled into one single circular chromosome with the size of 4,189,011 bp and G+C content of 52.

Marortus luteolus gen. nov., sp. nov., isolated from surface seawater of the East Sea in China.

A Gram-stain-negative, motile, aerobic, rod-shaped bacterium with flagella, designated ZX-21, was isolated from surface seawater of the East Sea in Zhoushan, China. Growth of strain ZX-21T was observed at 10--35  o°C (optimum, 30 °C), at pH 6.0-8.

Azoarcus pumilus sp. nov., isolated from seawater in Sanya, China.

A novel Gram-stain-negative, motile, rod-shaped (0.4-0.5×1.

Emcibacter congregatus sp. nov., isolated from sediment cultured in situ.

A Gram-negative, aerobic, motile, rod-shaped, pale-yellow bacterial strain, designated as ZYL, isolated from a cultured in situ sediment sample collected from the East China Sea coast, was studied using a polyphasic taxonomic approach. Strain ZYL grew at 4-30 °C (optimum, 25 °C), at pH 6.0-8.

Marinicaulis flavus gen. nov., sp. nov., a novel stalked bacterium of the family Parvularculaceae.

A Gram-stain-negative, aerobic, ovoid or short rod-shaped bacterium with prosthecates and flagella, designated SY-3-19, was isolated from the surface seawater of the South China Sea, and subjected to a polyphasic taxonomic study. The isolate grew at 4-40 °C and pH 5.0-9.

Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat.

A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7 was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7 grew at 4-40 °C and pH 6.0-9.

Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater.

A Gram-staining-negative, aerobic, non-spore-forming, coccoid to rod shaped bacteria with prosthecate and flagellum, designated as HSF6, was isolated from deep seawater samples collected from the South China Sea at depth of 2.5 km and subjected to a polyphasic taxonomic investigation. Colonies of strain HSF6 were 1-2 mm in diameter, smooth, circular, convex and yellow.

Mesorhizobium oceanicum sp. nov., isolated from deep seawater.

A novel Gram-staining-negative, oval-shaped (0.4-0.6×0.

Hyphobacterium vulgare gen. nov., sp. nov., a novel alphaproteobacterium isolated from seawater.

A Gram-stain-negative, aerobic, non-spore-forming, non-motile, oval to rod-shaped, prosthecate bacterium, designated strain WM6T, was isolated from a seawater sample collected from the South China Sea at a depth of 150 m and subjected to a polyphasic taxonomic investigation. Cells of strain WM6T were approximately 0.5-0.

Marinibaculum pumilum gen. nov., sp. nov., isolated from seawater.

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped strain, designed H2T, was isolated from the Western Pacific Ocean, and subjected to a taxonomic investigation using a polyphasic approach. Strain H2T grew at 15-40 °C and pH 6.0-9.

Marinobacterium zhoushanense sp. nov., isolated from surface seawater.

A Gram-stain-negative, facultatively anaerobic bacterium, designated WM3T, was isolated from surface seawater collected from the East China Sea. Cells were catalase- and oxidase-positive, short rods and motile by means of a single polar flagellum. Growth occurred at 15-43 °C (optimum 37-40 C), pH 5.

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci.

The members of the phylum Bacteroidetes are recognized as some of the most important specialists for the degradation of polysaccharides. However, in contrast to research on Bacteroidetes in the human gut, research on polysaccharide degradation by marine Bacteroidetes is still rare. The genus Algibacter belongs to the Flavobacteriaceae family of the Bacteroidetes, and most species in this genus are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae.

Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production.

In this study, a pyruvate carboxylase gene (PYC1) from a marine fungus Penicillium rubens I607 was cloned and characterized. ORF of the gene (accession number: KM397349.1) had 3534 bp encoding 1177 amino acids with a molecular weight of 127.

Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin.

In the present study, after the exo-inulinase gene INU1 from Meyerozyma guilliermondii was optimized according to the codon usage bias of Saccharomyces cerevisiae, both the optimized gene INU1Y and the native gene INU1 were ligated into the homologous integration expression vector pMIRSC11 and expressed in Saccharomyces sp. W0. It was determined that the inulinase activity of the recombinant yeast Y13 with the optimized gene INU1Y was 43.