Driver mutation landscape of acute myeloid leukemia provides insights for neoantigen-based immunotherapy.

Acute myeloid leukemia (AML) has lagged in benefiting from immunotherapies, primarily due to the scarcity of actionable AML-specific antigens. Driver mutations represent promising immunogenic targets, but a comprehensive characterization of the AML neoantigen landscape and their impact on patient outcomes and the AML immune microenvironment remain unclear. Herein, we conducted matched DNA and RNA sequencing on 304 AML patients and extensively integrated data from additional ∼2,500 AML cases, identifying 49 driver genes, notably characterized by a significant proportion of insertions and deletions (indels).

Mobilization and enrichment of geogenic iodine in a floodplain groundwater system: New insights from sources and characterization of dissolved organic matter.

High iodine groundwater occurs widely in the lower reaches of Yellow River floodplain, which has aroused public concern. The biogeochemical behavior of dissolved organic matter (DOM) plays a crucial role in the mobilizing iodine from aquifer media. In this study, the molecular composition of DOM in groundwater characterized by FT-ICR-MS, and the optical properties of organic matter obtained by combining three-dimensional fluorescence spectroscopy and parallel factor analysis (EEM ⁃ PARAFAC), were used to elucidate the effect of DOM on the migration and enrichment of iodine in groundwater in the eastern Henan Plain, which is located in the lower reaches of Yellow River floodplain, Northern China.

Effect of chitooligosaccharide on the binding domain of the SARS-COV-2 receptor.

The receptor-binding domain (RBD) is crucial for understanding how severe acute respiratory syndrome coronavirus (SARS-CoV-2) recognizes and infects host cells. Chitooligosaccharide (CS) exhibits diverse antiviral activities, with its derivatives showing remarkable efficacy in blocking SARS-CoV-2 infection. Thus, this study employed spectroscopy, virus-infected cell experiments, and molecular simulation to investigate the molecular interactions between CS and SARS-CoV-2 RBD, as well as their mechanisms.

Mutant U2AF1-Induced Mis-Splicing of mRNA Translation Genes Confers Resistance to Chemotherapy in Acute Myeloid Leukemia.

Unlabelled: Patients with primary refractory acute myeloid leukemia (AML) have a dismal long-term prognosis. Elucidating the resistance mechanisms to induction chemotherapy could help identify strategies to improve AML patient outcomes. Herein, we retrospectively analyzed the multiomics data of more than 1,500 AML cases and found that patients with spliceosome mutations had a higher risk of developing refractory disease.

The antipsychotic drug pimozide promotes apoptosis through the RAF/ERK pathway and enhances autophagy in breast cancer cells.

The antipsychotic drug pimozide has been demonstrated to inhibit cancer. However, the precise anti-cancer mechanism of pimozide remains unclear. The purpose of this study was to investigate the effects of pimozide on human MCF-7 and MDA-MB-231 breast cancer cell lines, and the potential involvement in the RAF/ERK signaling.

Silver Nanowires-Based Flexible Gold Electrode Overcoming Interior Impedance of Nanomaterial Electrodes.

In the development of nanomaterial electrodes for improved electrocatalytic activity, much attention is paid to the compositions, lattice, and surface morphologies. In this study, a new concept to enhance electrocatalytic activity is proposed by reducing impedance inside nanomaterial electrodes. Gold nanodendrites (AuNDs) are grown along silver nanowires (AgNWs) on flexible polydimethylsiloxane (PDMS) support.

Signal Processing and Waveform Re-Tracking for SAR Altimeters on High Mobility Platforms with Vertical Movement and Antenna Mis-Pointing.

Synthetic aperture radar (SAR) altimeters can achieve higher spatial resolution and signal-to-noise ratio (SNR) than conventional altimeters by Doppler beam sharpening or focused SAR imaging methods. To improve the estimation accuracy of waveform re-tracking, several average echo power models for SAR altimetry have been proposed in previous works. However, these models were mainly proposed for satellite altimeters and are not applicable to high-mobility platforms such as aircraft, unmanned aerial vehicles (UAVs), and missiles, which may have a large antenna mis-pointing angle and significant vertical movement.

Prophylactic supplement with melatonin prevented the brain injury after cardiac arrest in rats.

Prophylactic pharmacotherapy for health care in patients with high risk of cardiac arrest (CA) is an elusive and less explored strategy. Melatonin has possibilities used as a daily nutraceutical to trigger the cellular adaptation. We sought to find the effects of long-term daily prophylactic supplement with melatonin on the victim of CA.

A Sedentary Lifestyle Changes the Composition and Predicted Functions of the Gut Bacterial and Fungal Microbiota of Subjects from the Same Company.

A sedentary lifestyle affects the diversity and composition of the gut microbiota, but previous studies have mainly focused on bacteria instead of fungi. Here, we compared both the fecal bacterial and fungal microbiota compositions and functions in sedentary persons and controls. Subjects from the China Railway Corporation, including 99 inspectors and 88 officials, were enrolled in our study.

A comparative transcriptome analysis of how shrimp endure and adapt to long-term symbiosis with Enterocytozoon hepatopenaei infection.

Enterocytozoon hepatopenaei (EHP) is a prevalent microsporidian pathogen responsible for hepatopancreatic microsporidiosis (HPM) in Litopenaeus vannamei. This infection not only leads to slowed growth in shrimp abut aslo inflicts substantial economic losses in the global aquaculture industry. However, the molecular mechanisms by which EHP influences the host during various infection stages remain unclear.

Identification and validation of a novel CD8+ T cell-associated prognostic model based on ferroptosis in acute myeloid leukemia.

Acute myeloid leukemia (AML) is a highly aggressive cancer with great heterogeneity and variability in prognosis. Though European Leukemia Net (ELN) 2017 risk classification has been widely used, nearly half of patients were stratified to "intermediate" risk and requires more accurate classification excavating biological features. As new evidence showed that CD8+ T cell can kill cancer cells through ferroptosis pathway.

Measuring college belongingness: Structure and measurement of the Sense of Social Fit Scale.

Sense of belonging is theorized to be a fundamental human need and has been shown to have important implications in many domains of life, including academic achievement. The Sense of Social Fit scale (SSF; Walton & Cohen, 2007) is widely used to assess college belongingness, particularly to study differences in academic experiences along lines of gender and race. Despite its wide use, the instrument's latent factor structure and measurement invariance properties have not been reported in the published literature to date.

Intestinal Microbiota Differences in Shrimp between Greenhouse and Aquaponic Rearing.

The sustainability of shrimp aquaculture can be achieved through the development of greenhouse and aquaponic rearing modes, which are classified as heterotrophic and autotrophic bacterial aquaculture systems. However, there have been few investigations into the discrepancies between the intestinal and water microbiota of these two rearing methods. In this study, we collected shrimp samples from greenhouse-rearing (WG) and aquaponic-rearing (YG) ponds, and water samples (WE, YE), and investigated the intestinal and water microbiota between the two rearing modes.

Comparative transcriptome analysis of non-germinated and germinated spores of Enterocytozoon hepatopenaei (EHP) in vitro.

Enterocytozoon hepatopenaei (EHP), an obligate intracellular parasite classified as microsporidia, is an emerging pathogen with a significant impact on the global shrimp aquaculture industry. The understanding of how microsporidia germinate has been a key factor in exploring its infection process. However, the germination process of EHP was rarely reported.

Effect of chitooligosaccharide on the inhibition of SARS-CoV-2 main protease.

Background: The main protease (Mpro) is a crucial target for severe acute respiratory syndrome coronavirus (SARS-CoV-2). Chitooligosaccharide (CS) has broad-spectrum antiviral activity and can effectively inhibit the activity of SARS-CoV. Here, based on the high homology between SARS-CoV-2 and SARS-CoV, this study explores the effect and mechanism of CS with various molecular weights on the activity of SARS-CoV-2 Mpro.

Enhanced efficiency and mechanism of low-temperature biochar on simultaneous removal of nitrogen and phosphorus by combined heterotrophic nitrification-aerobic denitrification bacteria.

In this study, three strains of heterotrophic nitrification-aerobic denitrification (HN-AD) capable of simultaneously removing phosphorus were isolated from activated sludge, and low-temperature coconut shell biochar was prepared. The metabolic effects of combined HN-AD bacteria on the total nitrogen (TN) and total phosphorus (TP) were investigated, and the enhanced efficiency and mechanism of low-temperature biochar on the combined bacteria were also explored. The results indicated that the combined bacteria could adapt to environmental impacts and multiple nitrogen sources.

Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress.

Salinity is an important factor in the aquatic environment, and its fluctuations always result in osmotic stress, which affects the survival, distribution, and physiological activities of crustaceans. Crustaceans counter them through osmoregulation, which consists of many mechanisms. Palaemon gravieri is an important economic species in Palaemonidae, widely distributed in the southern East China Sea and the China Yellow Sea, and has a good adaptability to salinity stress.

The effects of dexmedetomidine for patient-controlled analgesia on postoperative sleep quality and gastrointestinal motility function after surgery: A prospective, randomized, double-blind, and controlled trial.

Postoperative poor sleep quality and decreased gastrointestinal motility function are common clinical problems. This study investigated the effects of dexmedetomidine (DEX) combined with sufentanil for patient-controlled analgesia (PCA) on postoperative sleep quality and gastrointestinal motility function after surgery in patients with colorectal cancer. Patients undergoing colorectal cancer surgery were randomly divided into three groups, DEX 0, 200, or 400 μg, each combined with sufentanil 150 μg for PCA immediately after surgery.

Preparation and characterization of sodium cellulose sulfate/chitosan composite films loaded with curcumin for monitoring pork freshness.

Colorimetric films were prepared by incorporating curcumin into a sodium cellulose sulfate/chitosan composite. The morphology mechanical, and water vapor properties of the films were investigated, and their practical use in pork preservation was evaluated. The formula with the same charge ratio of sodium cellulose sulfate and chitosan had the highest tensile strength (TS).

Effect of high-quality nursing on orthopedic trauma based on a fast-track surgery model.

Objective: To explore the effect of fast-track surgery (FTS) based high quality nursing on orthopedic trauma.

Methods: In this retrospective study, 94 patients who received orthopedic trauma surgery in our hospital from December 2018 to November 2020 were included. The patients were assigned to a research group (n=47) or a control group (n=47) according to which nursing method they received.

Rapid On-Site Detection Method for White Spot Syndrome Virus Using Recombinase Polymerase Amplification Combined With Lateral Flow Test Strip Technology.

The white spot syndrome virus is the most destructive virus threatening the shrimp industry worldwide, causing hundreds of millions of dollars in economic losses each year. There is currently no specific medicine to treat it. Therefore, rapid and accurate detection of WSSV is of great significance for controlling its spread and reducing economic losses.

Large-Scale In Vitro and In Vivo CRISPR-Cas9 Knockout Screens Identify a 16-Gene Fitness Score for Improved Risk Assessment in Acute Myeloid Leukemia.

Purpose: The molecular complexity of acute myeloid leukemia (AML) presents a considerable challenge to implementation of clinical genetic testing for accurate risk stratification. Identification of better biomarkers therefore remains a high priority to enable improving established stratification and guiding risk-adapted therapy decisions.

Experimental Design: We systematically integrated and analyzed the genome-wide CRISPR-Cas9 data from more than 1,000 in vitro and in vivo knockout screens to identify the AML-specific fitness genes.

Effect of quality control circle on nursing in orthopaedic trauma surgery.

Objective: To explore the application effect of Quality Management Circle (QCC) in nursing of orthopaedic trauma surgery.

Methods: The clinical data of 134 cases undergoing orthopaedic trauma surgery were assigned into 2 groups according to different nursing methods. Thereinto, 67 cases with traditional nursing were considered as the control group (CG), and the left with traditional nursing and QCC activities were assigned as the study group (SG).