Bioremediation of non-point hydrogen sulfide emissions using bacterial cellulose/activated carbon membrane.

Background: Hydrogen sulfide (HS) gas, characterized by its low odor threshold and toxicity, poses significant challenges in non-point source odor management. Traditional biotechnologies are effective in removing malodorous gases from point sources but they are limited for non-point source odor control.

Results: In this study, the sqr and pdo genes from Cupriavidus pinatubonensis JMP134 were introduced into the bacterial cellulose-producing strain Kosakonia oryzendophytica FY-07.

Prevalence and molecular spectrum of thalassemia in infertile population among different ethnic groups in Hainan Province, China.

Hainan is an area with high prevalence of thalassemia and complex genetic background. There are few studies on the prevalence and genotype of thalassemia in different ethnic groups of infertility patients in Hainan province. The aim of our study was to explore the prevalence and genotype of thalassemia among infertile individuals in Hainan Province.

Systematic mining and quantification reveal the dominant contribution of non-HLA variations to acute graft-versus-host disease.

Human leukocyte antigen (HLA) disparity between donors and recipients is a key determinant triggering intense alloreactivity, leading to a lethal complication, namely, acute graft-versus-host disease (aGVHD), after allogeneic transplantation. Moreover, aGVHD remains a cause of mortality after HLA-matched allogeneic transplantation. Protocols for HLA-haploidentical hematopoietic cell transplantation (haploHCT) have been established successfully and widely applied, further highlighting the urgency of performing panoramic screening of non-HLA variations correlated with aGVHD.

A Novel Motor Unit-Driven Neuro-Musculoskeletal Model for Estimating Continuous Wrist Motion.

Deciphering the intentions underlying movement based on motor unit (MU) activities poses an enduring challenge, thereby constraining our understanding of the intricate transition mechanism from microscopic neural commands to macroscopic movements. This investigation introduces an innovative MU-driven neuro-musculoskeletal (NMS) model, aimed at the continuous estimation of wrist movements. The presented model utilizes both MU firing and waveform information, incorporating a linear model and physiological twitch model for the computation of MU-specific neural excitations.

Enhancing the Myoelectric Pattern Recognition Robustness to Electrode Shift by an Autoencoder-Based Feature Calibrator.

Electrode shift is considered a pivotal and unavoidable factor affecting the robustness of myoelectric pattern recognition (MPR). Nevertheless, most existing solutions face challenges in simultaneously ensuring plug-and-play compatibility and achieving high classification accuracy. To address this challenge, this study presents a novel method for adaptively calibrating testing data samples using an autoencoder-based feature calibrator.

Deciphering the value of anoikis-related genes in prognosis, immune microenvironment, and drug sensitivity of laryngeal squamous cell carcinoma.

Background: Laryngeal squamous cell carcinoma (LSCC) is a prevalent malignancy characterized by high metastatic potential and poor prognosis. Anoikis, an apoptotic pathway triggered by detachment from the extracellular matrix (ECM), acts as a barrier against cancer metastasis, so it is necessary to explore the role of anoikis-related genes (ARGs) in LSCC.

Methods: Multivariate Cox regression analysis was used to construct prognostic model.

Liver-specific Bcl3 knockout alleviates acetaminophen-induced liver injury by activating Nrf2 pathway in male mice.

Background And Aims: Acetaminophen (APAP) overdose is the leading cause of acute liver failure, with oxidative stress being a critical factor in this process. Glutathione (GSH) plays a vital defensive role. Activation of Nrf2 pathway mitigates APAP-induced liver damage by promoting GSH biosynthesis and enhancing drug detoxification.

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models.

Dysregulation of mitochondrial function has been implicated in Parkinson's disease (PD), but the role of mitochondrial metabolism in disease pathogenesis remains to be elucidated. Using an unbiased metabolomic analysis of purified mitochondria, we identified alterations in α-ketoglutarate dehydrogenase (KGDH) pathway upon loss of PD-linked CHCHD2 protein. KGDH, a rate-limiting enzyme complex in the tricarboxylic acid cycle, was decreased in CHCHD2-deficient male mouse brains and human dopaminergic neurons.

HOXD8 drives Glioma progression through epithelial-mesenchymal transition regulation: Implications for prognosis and targeted therapy.

Glioma is one of the most common primary malignant tumors of the central nervous system. Here, we defined Homeobox D8 (HOXD8) as a novel biomarker for glioma utilizing RNA-sequencing and bioinformatics approaches. HOXD8 expression was significantly upregulated in glioma cell lines (U251 and U373) and clinical specimens compared to normal controls.

Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline.

Objective: Oral submucous fibrosis (OSF) is a chronic oral mucosal disease, which exerts a profound impact on patients' daily life and currently lacks efficacious therapeutic interventions. Epigallocatechin-3-gallate (EGCG), the abundant polyphenol found in green tea, exhibits remarkable anti-fibrotic effects on the skin. However, the research on OSF regarding EGCG is relatively limited.

Hypoxia-Activated Liposomes Enable Synergistic Photodynamic Therapy for Oral Cancer.

Oral cancer is a significant public health problem, which is one of the most common malignancy with high mortality in the world. Apart from surgery, photodynamic therapy (PDT) is perceived as another capable method for the treatment of oral cancer. However, limited oxygen content of tumor microenvironment decreased the efficiency of current PDT.

Next-generation sequencing based deep learning model for prediction of HER2 status and response to HER2-targeted neoadjuvant chemotherapy.

Introduction: For patients with breast cancer, the amplification of Human Epidermal Growth Factor 2 (HER2) is closely related to their prognosis and treatment decisions. This study aimed to further improve the accuracy and efficiency of HER2 amplification status detection with a deep learning model, and apply the model to predict the efficacy of neoadjuvant therapy.

Methods: We combined Next-Generation Sequencing (NGS) data and IHC staining images of 606 breast cancer patients and developed a Vision Transformer (ViT) deep learning model to identify the amplification of HER2 through these IHC staining images.

Learning Phenotype Associated Signature in Spatial Transcriptomics with PASSAGE.

Spatially resolved transcriptomics (SRT) is poised to advance the understanding of cellular organization within complex tissues under various physiological and pathological conditions at unprecedented resolution. Despite the development of numerous computational tools that facilitate the automatic identification of statistically significant intra-/inter-slice patterns (like spatial domains), these methods typically operate in an unsupervised manner, without leveraging sample characteristics like physiological/pathological states. Here PASSAGE (Phenotype Associated Spatial Signature Analysis with Graph-based Embedding), a rationally-designed deep learning framework is presented for characterizing phenotype-associated signatures across multiple heterogeneous spatial slices effectively.

A global gross primary productivity of sunlit and shaded canopies dataset from 2002 to 2020 via embedding random forest into two-leaf light use efficiency model.

Gross primary productivity (GPP) is crucial for understanding the carbon cycle and maintaining ecosystem balance under climate change. We attempt to generate a long-term global dataset for GPP of sunlit (GPP) and shaded leaves (GPP) by a hybrid model combining the random forest (RF) submodule with the two-leaf light use efficiency (TL-LUE) model. First, the TL-LUE model was optimized by considering the seasonal differences in the clumping index on a global scale (TL-CLUE).

Quantifying the regulatory potential of genetic variants via a hybrid sequence-oriented model with SVEN.

Deciphering how noncoding DNA determines gene expression is critical for decoding the functional genome. Understanding the transcription effects of noncoding genetic variants are still major unsolved problems, which is critical for downstream applications in human genetics and precision medicine. Here, we integrate regulatory-specific neural networks and tissue-specific gradient-boosting trees to build SVEN: a hybrid sequence-oriented architecture that can accurately predict tissue-specific gene expression level and quantify the tissue-specific transcriptomic impacts of structural variants across more than 350 tissues and cell lines.

RTEL1 is upregulated in gastric cancer and promotes tumor growth.

Gastric cancer (GC) is one of the most common cancers worldwide, with increasing incidence and mortality rates. It is typically diagnosed at advanced stages, leading to a poor prognosis. GC is a highly heterogeneous disease and its progression is associated with complex interplay between genetic and environmental factors.

Regulation mechanism of the long-chain -alkane monooxygenase gene in RAG-1.

Unlabelled: As toxic pollutants, -alkanes are pervasively distributed in most environmental matrices. Although the alkane monooxygenase AlmA plays a critical role in the metabolic pathway of solid long-chain -alkanes (≥C) that are extremely difficult to degrade, the mechanism regulating this process remains unclear. Here, we characterized the function of AlmA in RAG-1, which was mainly involved in the degradation of long-chain -alkanes (C-C), among which, -C induced the promoter activity most.

Effect of Leaf Powder on Growth Performance, Diarrhea Incidence, Blood Biochemistry, and Intestinal Traits in Weaned Piglets.

To evaluate the phytobiotic effect of leaf powder (AOLP) on growth performance, diarrhea incidence, blood biochemistry, and intestinal traits, seventy-two weaned piglets were randomly distributed into four groups (six replicates/group and three pigs/replicate) for 28 days, receiving a control diet (T0) or being supplemented with 5 (T1), 10 (T2), or 15 (T3) g/kg of AOLP. The diets did not affect the growth performance ( > 0.05); however, the AOLP groups had a decreased diarrhea incidence and malondialdehyde concentration ( < 0.

Activated TREM1-mediated MAPK signaling in endothelial cells caused by highly expressed STAT1 is associated with intracranial aneurysms occurrence and rupture.

Intracranial aneurysm (IA) poses significant health risks, yet the specific mRNA profiles and regulatory mechanisms distinguishing unruptured IA (UIA) from ruptured IA (RIA) remain unclear. This study aimed to elucidate these differences through comprehensive mRNA analysis. We employed RNA sequencing to compare mRNA expression patterns among control individuals, UIA patients, and RIA patients.

Association and Diagnostic Value of TyG-BMI for Hyperuricemia in Patients with Non-Alcoholic Fatty Liver Disease: A Cross-Sectional Study.

Purpose: This study aimed to investigate the association between TyG-BMI and hyperuricemia in NAFLD patients and assess its potential diagnostic value compared to the TyG index.

Patients And Methods: This study selected the patients diagnosed with fatty liver disease at the Affiliated Hospital of Chengde Medical University between September and December 2023. These patients were divided into NAFLD without HUA (NAFLD-NUA, n=1166) and NAFLD with HUA (NAFLD-HUA, n=844) groups.

Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood-Brain Barrier Properties.

Chronic neuroinflammation is a principal cause of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. The blood-brain barrier predominantly comprises endothelial cells, and their intercellular communication with pericytes and other cell types regulates neuroinflammation. Here, we develop a tubular, perfusable model of human cerebrovascular tissues to study neurodegenerative diseases using cerebrovascular-specific extracellular matrix bioink, derived from a complementary blend of brain- and blood-vessel-derived extracellular matrices.

High Incubation Attendance and Nesting Site Constraints of the Sclater's Monal in an Alpine Environment in Southwestern China.

Breeding in alpine environments poses significant challenges to birds, requiring specific adaptations for survival. The Sclater's monal (), a regionally threatened, typical alpine pheasant species, is restricted to high-elevation habitat from the East Himalayas to the mountains of west Yunnan, China. Due to its low population density and the difficulty of accessing its habitats, the breeding ecology of this species is understudied.

Bioengineered human arterial equivalent and its applications from vascular graft to disease modeling.

Arterial disorders such as atherosclerosis, thrombosis, and aneurysm pose significant health risks, necessitating advanced interventions. Despite progress in artificial blood vessels and animal models aimed at understanding pathogenesis and developing therapies, limitations in graft functionality and species discrepancies restrict their clinical and research utility. Addressing these issues, bioengineered arterial equivalents (AEs) with enhanced vascular functions have been developed, incorporating innovative technologies that improve clinical outcomes and enhance disease progression modeling.

A novel function for α-synuclein as a regulator of NCK2 in olfactory bulb: implications for its role in olfaction.

To investigate physiological function of α-synuclein is important for understanding its pathophysiological mechanism in synucleinopathies including Parkinson's disease. Employing knockout mice, we found that Snac/α-synuclein deletion induced aberrant projection of olfactory sensory neurons and hyposmia. We identified 9 axon guidance associated differentially expressed proteins using iTRAQ based Liquid Chromatograph Mass Spectrometer.