Low-Energy Traumatic Obturator Hip Dislocation with Ipsilateral Femoral Shaft Fracture in a Patient with Omolateral Knee Arthroplasty.

Ipsilateral obturator hip dislocation and femoral shaft fracture are rare. We report such a case in an older woman after a low-energy injury. She had a knee prostheses in the same limb.

Successful surgical treatment of a four part fracture dislocations of the proximal humerus and coracoid avulsion.

racture-dislocation of the proximal humerus (usually occurring after violent trauma) may be more frequently associated with a poor long-term results because the destruction of the hinged periosteum is associated with an high-risk of avascular necrosis of the head of the humerus (1, 2). Concomitant coracoid fracture with anterior shoulder dislocation in such case is something extremely rarely reported (8). Herein, we describe a challenging case of a 44 years old man with 4-parts fracture of the right proximal humerus, dislocation of the glenohumeral joint associated with coracoid avulsion.

Causes and treatments of lag screw's cut out after intramedullary nailing osteosinthesis for trochanteric fractures.

Background. Superior cut-out of a lag screw remains a serious complication in the treatment of trochanteric or subtrochanteric fractures and it is related to many factors: the type of fracture, osteoporosis and the stability of fracture reduction. Little is known about the outcome after revision surgery for complications of the gamma nail.

Hemiarthroplasty versus reverse shoulder arthroplasty: comparative study of functional and radiological outcomes in the treatment of acute proximal humerus fracture.

Purpose: To compare functional and radiographic results of reverse prosthesis versus hemiarthroplasty after complex displaced proximal humeral fractures in elderly patients when adequate ORIF cannot be achieved and prosthetic shoulder replacement is required.

Methods: From 2008 to 2012, 67 patients were treated with hemiarthroplasty or reverse arthroplasty. We evaluated 53 cases with an average follow-up of 27.

Distal radius articular fractures: a comparison between ORIF with angular stability plate and percutaneous Kirschner wires.

Purpose Of The Study: To analize and compare vantages and disadvantages in long-term of two different  techniques to treat distal radius articular fractures: ORIF with plate versus percutaneous pinning with K-wires.

Materials And Methods: We reviewed 77 distal radial articular fractures treated surgically from 2005 to 2009. Fractures were divided in two homogeneous groups according to patient age, gender, fracture-type and follow-up.

Displaced mid-shaft clavicular fractures: surgical treatment with a pre-contoured angular stability plate.

The treatment for displaced mid-shaft clavicle fracture is highly controversial. In the last years, several biomechanical studies showed better functional results after surgical treatment. The purpose of this study is to evaluate the use of pre-contoured angular stability plate in this type of fracture.

[Recurrent acute myocardial infarction in a patient with nocturnal paroxysmal hemoglobinuria].

Paroxysmal nocturnal hemoglobinuria is a form of acquired hemolytic anemia with a high incidence of thrombotic complications, generally in the venous district; arterial thrombosis is rare, and exceptional in the coronary tree. We describe the case of a man who had two episodes of myocardial infarction, both during a hemoglobinuric crisis; this patient was free from cardiovascular risk factors and angiography revealed that there was no coronary stenosis. The clinical course was favorable and the patient's response to thrombolytic and dicumarol therapy was satisfactory.

[Limits of cardiac functional adaptation in "top level" resistance athletes].

Background: Sports activity, particularly when performed at high level, provokes cardiovascular adjustments depending on the type of sport and on the level of the load.

Methods: We evaluated 15 athletes from the Italian national team during a non-agonistic period of cross country skiing, with non-invasive tests including exercise test, color Doppler echocardiography, Holter monitoring, physical examination and standard rest electrocardiogram.

Results: Physical examination, rest electrocardiogram, exercise testing and echocardiography were all within the range of the expected values for this type of subjects.

[Possibility of evaluation of chest pain at the time of hospitalization].

A series of 140 patients admitted to an Internal Medicine or a Cardiology Department for an acute chest pain is examined in order to evaluate the possibility of reaching a quick diagnosis, particularly for coronaric heart diseases, according with history, physical examination, electrocardiogram. In most of cases it was possible an immediate, correct evaluation of the patients, but in some cases a right diagnosis was achieved only after a period of observation and different investigations.

New trends in ganglioside chemistry.

New methods have been developed for the preparation of highly purified gangliosides, homogeneous in the saccharide, long chain base, and fatty acid moieties and gangliosides carrying different kinds of labelled probes. Gangliosides, homogeneous in the oligosaccharide portion, were prepared by preparative normal phase HPLC on a Lichrosorb-NH-2 column, using a gradient of acetonitrile-phosphate buffer, pH 5.6, as solvent system.

[Bone metastasis as a sign of the existence of latent neoplasia].

A series of 65 patients in whom bone metastasis was the earliest sign of tumour is examined. The diagnostic aspects of the bone metastasis from laboratory examination to radiology, from scintigraphy to bone marrow biopsy are analysed as is the problem of locating the primary tumours that remain of unknown site. The prognosis is poor for all such patients.

Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures.

A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through detection of UV absorbance at 215 nm.

High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous long chain base composition.

A semi-preparative, analytical high performance liquid chromatographic (HPLC) procedure is described for the isolation of molecular species of GM1 and GD1a gangliosides containing a single long chain base, C18 or C20 sphingosine, C18 or C20 sphinganine, each in its natural erythro or unnatural threo form. The threo forms were obtained from 2,3-dichloro-5,6-dicyanobenzoquinone/NaBH4 -treated gangliosides. The ganglioside molecular species separated by HPLC were analyzed for carbohydrate, fatty acid, and long chain base composition.

Analytical and preparative high-performance liquid chromatography of gangliosides.

Analytical and preparative procedures are described for high-performance liquid chromatography (HPLC) fractionation of gangliosides without previous derivatization. These procedures make use of a reversed-phase Lichrosorb RB-8 or mu Bondapak RP-18 column, and of a mixture of acetonitrile and 5 mM phosphate buffer, at fixed or varying volume ratios, as solvent system. Peak elution from the column is monitored by flow through reading of absorbance at 195 nm.

Perinatal development of styrene monooxygenase and epoxide hydrolase in rat liver microsomes and nuclei.

Nuclear enzymes were found to develop earlier than the corresponding microsomal activities. In fact styrene monooxygenase enzymatic activity at 18 days gestational age reached about half the values of adult animals, whereas fetal microsomal activity was only about 1/20 the adult level at the same age. In microsomes and nuclei the ontogenic development of epoxide hydrolase is slightly slower than styrene monooxygenase.

Opiate tolerance and dependence is associated with a decreased activity of GTPase in rat striatal membranes.

In vitro addition of opiates to rat striatal membranes significantly stimulated a low Km GTPase activity. The opioid peptide (D-Ala2, Met5) enkephalinamide was ten folds more active than morphine to elicit the effect while the kappa agonist ethylketocyclazocine was almost inactive. Opiate stimulation was antagonized by naloxone, indicating that specific opiate receptors were involved.

Nuclear metabolism. II. Further studies on epoxide hydrolase activity.

Apparent Km- and Vmax-values of nuclear styrene 7,8-oxide hydrolase were determined at different protein concentrations. In the protein concentrations range used no significant differences in the apparent Km-values were observed. The influence of the incubation with different modifiers (i.

Induction of nuclear styrene monooxygenase and epoxide hydrolase in rat liver.

The apparent Km and Vmax of styrene monooxygenase and styrene epoxide hydrolase were determined in intact nuclear preparations from male rat liver after in vivo treatment with phenobarbital and beta-naphthoflavone, which are known to induce microsomal cytochrome P-450 and cytochrome P-448 respectively. Treatment with phenobarbital does not alter the apparent Km, but greatly increases the Vmax of both nuclear styrene monooxygenase and styrene epoxide hydrolase. Almost the same pattern is observed for styrene monooxygenase after treatment with beta-naphthoflavone, whereas the same treatment slightly increases both the Vmax and Km value of styrene epoxide hydrolase.

Is nuclear styrene monooxygenase activity a microsomal artifact?

Microsomal contamination in nuclear preparations could represent one of the main sources of bias in the evaluation of the real metabolic capacity of the nuclear envelope. In this paper we present a quantitative study of the level of nuclear styrene monooxygenase enzymatic activity after artificially increasing the native microsomes to nuclei ratio. In one experimental conditions no significant elevation of the nuclear monooxygenase was observed.

Nuclear metabolism. I. Determination of styrene monooxygenase activity in rat liver nuclei.

Styrene monooxygenase activity was measured in intact nuclear preparations from rat liver by means of a gas chromatographic method. Styrene epoxide formation is NADPH-dependent although it is enhanced when NADH is added with NADPH. This activity is inhibited by microsomal monooxygenase inhibitors SKF 525A and metyrapone and by microsomal epoxide hydrase inhibitors 1,2-epoxy-3,3,3-trichloropropene oxide and cyclohexene oxide.