A new method for studying protein interactions called improved Bimolecular Fluorescence Complementation (BiFC) has been developed, focusing on better detection of protein expression levels.
This method uses a bicistronic system that links the protein of interest with a fluorescent marker through a self-cleaving peptide, allowing for more accurate identification of interactions.
Interestingly, the study also reveals that a previously identified non-interacting protein pair is actually interacting when observed in live cells, demonstrating the method's effectiveness.