Publications by authors named "Gayane V Ananyan"
A study of rats liver DNA damages under the influence of X-ray radiation at a dose of 6.5 Gy(LD60) was carried out. The radioprotective properties of newly synthesized Cu(II) L-Schiff Histidinate complexes were also studied.
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- The study investigates how a water-soluble porphyrin, HTOEtPyP4, and its manganese derivative, MnTOEtPyP4, bind to calf thymus DNA using various scientific techniques including UV/Vis spectrophotometry and Circular Dichroism.
- Results indicate that at low concentrations, porphyrins engage with DNA through one binding mode, while at higher concentrations, they display two distinct binding modes.
- Thermal melting analysis reveals that these porphyrins help stabilize the double-helical structure of DNA, and evidence of different melting curves suggests varying interactions based on concentration.
The structural features of poly(dG-dC).poly(dG-dC) at complexation with some porphyrins.
J Biomol Struct Dyn
December 2022
Article Synopsis
- The study investigates how three porphyrins (HTOEtPyP4, CuTOEtPyP4, and CoTOEtPyP4) interact with a synthetic polynucleotide, poly(dG-dC).poly(dG-dC), using UV/Vis absorption and circular dichroism methods.
- The results show that HTOEtPyP4 and CuTOEtPyP4 can intercalate into the polynucleotide at low concentrations, while at higher concentrations, they also bind externally; however, CoTOEtPyP4 can only bind externally due to its non-planar structure.
- Binding constants were calculated for each porphyrin, indicating that HTOEtPyP4 has
pH-dependent complex formation of Zn-meso-tetra(4-hydroxyethylpyridyl) porphyrin with cancer DNA.
J Biomol Struct Dyn
February 2021
The complex formation between the synthetic water-soluble Zn--tetra(4hydroxyethylpyridyl) porphyrin (ZnTOEPyP4) and cancer DNA in comparison to healthy DNA was investigated using the UV/VIS spectrophotometry method in phosphate-buffered saline at different pHs. The increasing of DNA/porphyrin ratio leads to hypochromicity and red shift in the Soret band, which indicate the complexation of the ZnTOEPyP4 with DNA. The results show that the binding constant () and the exclusion parameter () of ZnTOEPyP4 with DNA strongly depend upon the pH.
View Article and Find Full Text PDFDevelopment of viscometric methods for studying the interaction of porphyrins with DNA.
J Biomol Struct Dyn
August 2020
The present paper is an overview of studying of DNA-porphyrin interactions using viscometry in combination with the spectroscopic methods. It was shown, that when porphyrins interact with DNA as an outside binder, the interaction mode and intensity does not depend on metal center, peripheral substituent's and their positions on pyridylic ring. In case of planar porphyrins, the binding type is mainly determined by type of peripheral substituent's and their position on the pyridylic ring.
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