Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation.

Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples.

Origin of CMS-PET1 cytotype in cultivated sunflower: A new insight.

The vast majority of commercial sunflower hybrids worldwide are produced using cytoplasmic male sterility (CMS) of the PET1 type, resulting from the interspecific hybridization of Helianthus petiolaris with Helianthus annuus. Due to the fact that CMS-PET1 was not previously detected in wild sunflower, it was believed that this cytotype could arise during interspecific hybridization and is specific solely for cultivated sunflower. In this study, the open reading frame, orfH522, associated with the CMS-PET1 phenotype, was revealed for the first time in the 3'-flanking region of the mitochondrial atpA gene in wild H.

Mitogenomic Research of Silverleaf Sunflower () and Its Interspecific Hybrids.

Interspecific hybridization is widespread for sunflowers, both in wild populations and commercial breeding. One of the most common species that can efficiently cross with is the Silverleaf sunflower-. The current study carried out structural and functional organization analyses of mitochondrial DNA in and the interspecific hybrid, (VIR114A line) × .

[Analysis of the whole-genome sequence of an ASF virus (Asfarviridae: Asfivirus: African swine fever virus) isolated from a wild boar (Sus scrofa) at the border between Russian Federation and Mongolia].

Introduction: The causative agent of African swine fever (Asfarviridae: Asfivirus: African swine fever virus) (ASF) is a double-stranded DNA virus of 175-215 nm. To date, 24 of its genotypes are known. Clustering of ASF genotype II isolates is carried out by examining a limited number of selected genome markers.

The Insights into Mitochondrial Genomes of Sunflowers.

The significant difference in the mtDNA size and structure with simultaneous slow evolving genes makes the mitochondrial genome paradoxical among all three DNA carriers in the plant cell. Such features make mitochondrial genome investigations of particular interest. The genus is a diverse taxonomic group, including at least two economically valuable species-common sunflower () and Jerusalem artichoke ().

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content.

Background: Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding.

Data on the polymorphic sites in the chloroplast genomes of seven perennial species.

Data present the chloroplast genome sequences of seven wild perennial species obtained by using the Illumina HiSeq and NextSeq platforms. Datasets not included in the primary publication [1] are a source for further evolutionary studies. In particular, the annotated chloroplast genomes and datasets of single nucleotide polymorphisms (SNP), simple sequence repeats (SSR), insertion and deletion polymorphisms (INDEL) for , and are presented.

Comparative analysis of chloroplast genomes of seven perennial Helianthus species.

Sequencing and a comparative analysis of the complete chloroplast genomes of seven perennial Helianthus species were carried out. The chloroplast genomes have a typical quadripartite structure, including large and small single regions and a pair of inverted repeats. Genome sizes were between 151,152 bp and 151,289 bp.

The Investigation of Perennial Sunflower Species ( L.) Mitochondrial Genomes.

The genus is a diverse taxonomic group with approximately 50 species. Most sunflower genomic investigations are devoted to economically valuable species, e.g.

Organization Features of the Mitochondrial Genome of Sunflower ( L.) with ANN2-Type Male-Sterile Cytoplasm.

This study provides insights into the flexibility of the mitochondrial genome in sunflower ( L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies.

Data on the polymorphic sites in the chloroplast genomes of the sunflower alloplasmic CMS lines.

Data presents the chloroplast genome sequences of the five sunflower alloplasmic cytoplasmic male sterility (CMS) lines obtained with using the Illumina MiSeq, HiSeq and NextSeq platforms. The sunflower alloplasmic CMS lines has the same nuclear genome from line HA89, but they differ in cytoplasmic genomes, inherited from annual (PET1, PET2 - , ANN2 - ) and perennial (MAX1 - ) species of the genus L. The chloroplast genomes were annotated.

Characterization of the mitochondrial genome of the MAX1 type of cytoplasmic male-sterile sunflower.

Background: More than 70 cytoplasmic male sterility (CMS) types have been identified in Helianthus, but only for less than half of them, research of mitochondrial organization has been conducted. Moreover, complete mitochondrion sequences have only been published for two CMS sources - PET1 and PET2. It has been demonstrated that other sunflower CMS sources like MAX1, significantly differ from the PET1 and PET2 types.

Mitochondrial genomes organization in alloplasmic lines of sunflower ( L.) with various types of cytoplasmic male sterility.

Background: Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, that is often associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. Investigation of the CMS phenomenon promotes understanding of fundamental issues of nuclear-cytoplasmic interactions in the ontogeny of higher plants. In the present study, we analyzed the structural changes in mitochondrial genomes of three alloplasmic lines of sunflower ( L.

[Study of Chloroplast DNA Polymorphism in the Sunflower (Helianthus L.)].

The polymorphism of microsatellite loci of chloroplast genome in six Helianthus species and 46 lines of cultivated sunflower H. annuus (17 CMS lines and 29 Rf-lines) were studied. The differences between species are confined to four SSR loci.

[Polymorphism among RFL-PPR homologs in sunflower (Helianthus annuus L.) lines with varying ability for the suppression of the cytoplasmic male sterility phenotype].

A complex comparative genetic approach was used for the investigation of the structural and functional diversity of genes for the restoration of sunflower pollen fertility. It includes (i) hybridological analysis; (ii) analysis of polymorphism among EST fragments.homologous to the known Rf genes that contain repeated motives of 35 amino acids (RFL-PPR); (iii) the development of molecular markers.

[Association of (N251S)-PIP5K2A with schizophrenic disorders: a study of the Russian population of Siberia].

The phosphatidylinositol-4-phosphate-5-kinase type IIa (PIP5K2A) gene has been proposed as a putative susceptibility gene for schizophrenia on both positional and functional grounds. The association between the (N251S)-PIP5K2A (rs10828317) variant and schizophrenia was found in German and Dutch populations but was not replicated in several other populations. The purpose of the study was to examine whether the previously implicated (N251S)-PIP5K2A variant influences susceptibility to schizophrenia in the Russian population of Siberia.

Neurosteroids dehydroepiandrosterone and its sulfate in individuals with personality disorders convicted of serious violent crimes.

We studied blood serum levels of neurosteroids, dehydroepiandrosterone and its sulfate, in individuals with personality disorders convicted of serious violent crimes. The data were compared with that of a group of mentally and physically healthy persons convicted of acquisitive crimes, and with that of the control group. Significant increase in DHEA in both groups of convicts in comparison with the control was shown.

Separation, characterization and quantification of phenolic compounds in blueberries and red and black currants by HPLC-DAD-ESI-MSn.

The phenolic profile of four blueberry varieties (Vaccinium corymbosum L., cv. Toro, Legacy, Duke and Bluecrop) and two varieties (Rosenthal and Rovada) of red currants (Ribes rubrum L.

[Genomic instability in sunflower interspecific hybrids].

The expression of genomic instability was studied at the phenotypical (morphological characters, electrophoretic spectra of seed storage proteins) and molecular (DNA amplification products) levels in interspecific hybrids (ISHs) from crosses of inbred lines of cultivated sunflower Helianthus annuus with perennial species of the genus Helianthus and in introgressive lines (ILs) produced on their basis. Unstable state of the locus determining the trait of lower branching was proved by the method of hybridological analysis. It was shown with the use of RAPD markers that the IL genome is characterized by instability even after long-term inbreeding (in generations F8-F12).

[Gingival tissues age- and gender-specific peculiarities in healthy conditions and in cases of chronic apical periodontitis].

By light microscopy there was studied gingival tissues structure depending upon age and gender in 96 relatively healthy persons and 80 patients with chronic apical periodontitis. It was disclosed that in gingival mucosa of patients with chronic apical periodontitis the interstitial space volumetric density and numerical density of tissue leucocytes increased in the first place due to neutrophils. Between the healthy men and women there was no significant difference in structural organization of the gum as well as between the patients of different gender.

[Periodontal tissues status after treatment of chronic periodontitis with consideration of gender and age].

Radiovisiographic study of periodontal tissues changes in different periods after the therapy of destructive chronic periodontitis have disclosed that jaw alveolar process bone density and the size of the destruction focus after 6 months, 1 and 2 years after the treatment were not significantly changed (134 patients sample of different gender and age). When this sample was subdivided into groups depending upon gender and age some significant changes of some parameters were found. The most expressed were the changes of the size of the periodontal destruction focus in the patients of 40-49 years of age, less pronounced they were in patients of 18-29 age group; in the age group 30-39 years changes were not found, In men of 18-29 age group regenerative processes in periodontal tissues were more expressed than in women.

Laccase and Mn-peroxidase production by Coriolus hirsutus strain 075 in a jar fermentor.

The white-rot fungus Coriolus hirsutus strain 075 excretes considerable amounts of laccase and Mn-peroxidase into culture broth over a brief production time. The effects of agitation speed, temperature, aeration and inoculum amount on laccase production using a 10-l fermentor were studied. The optimum fermentation conditions were a 15% inoculum, an aeration rate of 0.

[Polymorphism and inheritance of seed storage protein in sunflower].

The data on polymorphism and inheritance of the seed storage protein helianthinin are presented. The results of hybrid analysis indicate that in the annual sunflower Helianthus annuus, helianthinin synthesis is controlled by at least three loci: HelA, HelB, HelB, and HelC. Codominant alleles controlling different electrophoretic variants of polypeptides were identified at each of the loci.