Effect of Plasma Unit Weight and Donor Sex on Post-Donation Citrate Level: An Experimental Study on Plasmapheresis Donors.

Introduction: Plasmapheresis donation is considered safe and well tolerated, although long-term effects need to be clarified. The volumes of anticoagulant (ACD-A) used are variable and depend primarily on hematocrit (HCT), total blood processed, amount of plasma collected, and donor characteristics. To elucidate the effect of the plasma unit weight setting on plasmapheresis efficiency and ACD-A distribution, we enrolled male donors undergoing a controlled apheresis process donating 700 g and 720 g in two different sessions.

Cryopreservation affects platelet macromolecular composition over time after thawing and differently impacts on cancer cells behavior in vitro.

Cryopreservation affects platelets' function, questioning their use for cancer patients. We aimed to investigate the biochemical events that occur over time after thawing to optimize transfusion timing and evaluate the effect of platelet supernatants on tumor cell behavior . We compared fresh (Fresh-PLT) with Cryopreserved platelets (Cryo-PLT) at 1 h, 3 h and 6 h after thawing.

A cohort study on the biochemical and haematological parameters of Italian blood donors as possible risk factors of COVID-19 infection and severe disease in the pre- and post-Omicron period.

To investigate the association between biochemical and blood parameters collected before the pandemic in a large cohort of Italian blood donors with the risk of infection and severe disease. We also focused on the differences between the pre- and post-Omicron spread in Italy (i.e.

Reduction of EpCAM-Positive Cells from a Cell Salvage Product Is Achieved by Leucocyte Depletion Filters Alone.

Intraoperative cell salvage reduces the need for allogeneic blood transfusion in complex cancer surgery, but concerns about the possibility of it re-infusing cancer cells have hindered its application in oncology. We monitored the presence of cancer cells on patient-salvaged blood by means of flow cytometry; next, we simulated cell salvage, followed by leucodepletion and irradiation on blood contaminated with a known amount of EpCAM-expressing cancer cells, assessing also residual cancer cell proliferation as well as the quality of salvaged red blood cell concentrates (RBCs). We observed a significant reduction of EpCAM-positive cells in both cancer patients and contaminated blood, which was comparable to the negative control after leucodepletion.

Mother-newborn ABO group discrepancy caused by a rare BW.17 variant.

Several ABO gene mutations are known to determine rare subgroups: these ABO variants are often responsible for weak or null phenotypes and may cause an incorrect determination of the serotype. Here we describe for the first time the phenotypic discrepancy of a rare B allele within the same Caucasian family that depends on the co-inheritance with A or H antigen. Blood samples from newborns, mothers, and grandmothers were analysed through routine serotype and genotype testing.

Platelet-rich Plasma Lysate for Treatment of Eye Surface Diseases.

Various ocular surface diseases are treated with blood-derived eye drops. Their use has been introduced in clinical practice because of their metabolite and growth factor content, which promotes eye surface regeneration. Blood-based eye drops can be prepared from different sources (i.

Empirical modeling and simulation of phase noise in long-haul coherent optical transmission systems.

An empirical phase noise channel model suitable for performance evaluation of high spectrally efficient modulations in 100G long-haul coherent optical transmission systems using polarization-division multiplexed and wavelength-division multiplexing channels is presented. The derivation of the model is worked out by exploiting the similarity between the power spectral density of the carrier extracted from the analysis of propagation measurements and the Lorentzian spectrum that is usually adopted to describe instabilities of semiconductor lasers. The proposed channel model is characterized by only two parameters: the linewidth of the carrier and the signal-to-noise ratio.

PDM-16QAM transmission performance over uncompensated fiber links.

The dependence of propagation performance on the signal baud-rate is investigated by simulations for WDM PDM-16QAM systems operating at a spectral efficiency of 4 b/s/Hz. We take into account the case of transmission over uncompensated links, both for standard single-mode fiber and non-zero dispersion-shifted fiber. Three baud-rates are tested: 16.

Experimental and numerical investigation of bit-wise phase-control OTDM transmission.

The impact of varying the phase relationship between adjacent OTDM channels is investigated in 80 Gbit/s transmission experimentally and numerically. A fiber-based coherent multiplexer is proposed for OTDM experiments - a phase shifter in the multiplexer and an external phase control circuit are used to set and maintain the phase difference. It is demonstrated that the optimum modulation format for maximum transmission distance strongly depends on pulse width, e.

Experimental investigation of SPM in long-haul direct-detection OFDM systems.

We experimentally investigate the effect of self-phase modulation on direct-detection orthogonal frequency division multiplexed (OFDM) transmission at 11.1 Gb/s over 960km and 1600km uncompensated standard single-mode fiber links. We show that for long-haul systems, the penalties due to nonlinear distortion in OFDM systems are comparable to those in links employing electronic predistortion.

Electronic compensation of chromatic dispersion using a digital coherent receiver.

Digital signal processing (DSP) combined with a phase and polarization diverse coherent receiver is a promising technology for future optical networks. Not only can the DSP be used to remove the need for dynamic polarization control, but also it may be utilized to compensate for nonlinear and linear transmission impairments. In this paper we present results of a 42.

Theoretical and electrochemical analysis of dissociative electron transfers proceeding through formation of loose radical anion species: reduction of symmetrical and unsymmetrical disulfides.

The dissociative reduction of a series of symmetrical (RSSR, R = H, Me, t-Bu, Ph) and unsymmetrical disulfides (RSSR', R = H, R' = Me and R = Ph, R' = Me, t-Bu) was studied theoretically, by MO ab initio calculations and, for five of them, also experimentally, by convolution voltammetry in N,N-dimethylformamide. The reduction is dissociative but proceeds by a stepwise mechanism entailing the formation of the radical anion species. The electrochemical data led to estimated large intrinsic barriers, in agreement with an unusually large structural modification undergone by the disulfide molecules upon electron transfer.

Ozonation of blood during extracorporeal circulation. I. Rationale, methodology and preliminary studies.

We investigated whether exposure of blood ex-vivo to oxygen-ozone (O2-O3) through a gas exchanger is feasible and practical. We first evaluated the classical dialysis-type technique but we soon realized that semipermeable membranes are unsuitable because they are hydrophilic and vulnerable to O3. We therefore adopted a system with hydrophobic O3-resistant hollow fibers enclosed in a polycarbonate housing with a membrane area of about 0.