Anthropometry of Mexican-American migrant children in northern California.

Anthropometric data were obtained from 209 Mexican-American migrant children, aged 0 to 7 yr, in the Sacramento Valley of California. Hematocrit was measured for 170 children. Only 13 children (7.

The determination of the specific activity of plasma glycerol.

A method for the determination of the specific activity of plasma glycerol is described. Anionic contaminants are first removed from deproteinzed plasma by anionic exchange resins (treated plasma). Glycerol in treated plasma is then quantitatively converted to glycerol-3-phosphate (G3P), which is isolated by column chromatography and counted for 14C radio-activity.

Turnover studies of human beta 2-microglobulin in the rat: evidence for a beta 2-microglobulin-binding plasma protein.

1. 125I-labelled beta 2-microglobulin (beta 2m) was injected into rats and protein or non protein-bound radioactivity was determined in plasma urine and several organs. 2.

Piretanide and urinary excretion of ions in rat. Effect of indomethacin.

1. Piretanide is a new loop diuretic resembling furosemide. The effects of this diuretic on various parameters of renal function and on the excretion of the major ions were studied on anaesthetized, hydrated rats.

A three step purification procedure for human liver ferritin.

We describe a method for the purification of normal human liver ferritin by ultrafiltration, gel filtration on Sephacryl S-300, and affinity chromatography on DEAE-Affi Gel Blue. The purity of the ferritin obtained was verified by immunoelectrophoresis, Ouchterlony immuno-diffusion, polyacrylamide gel electrophoresis, and electrofocusing. This rapid method yields 32% of the original ferritin.

Yeast, rye, and calf histones. Similarities and differences detected by electrophoretic and immunological methods.

Yeast histones H2A, H2B, and H3 were purified using the standard histone purification procedures of differential solubility and exclusion chromatography. Yeast histone H4 was isolated by the same methods in a fraction containing one other major protein component. The four yeast core histones were identified by their reactions with antisera against rye and (or) calf histone fractions as well as by their electrophoretic, chromatographic, and solubility properties.

Effect of acidosis, alkalosis and monofluoroacetate administration on citrate and ATP content of rat renal medulla and papilla.

1. --Renal distribution of citrate showed that there is an increase in citrate content from cortex to medulla and a decrease from medulla to papilla. Alkalosis produced an increase in citrate content and acidosis a decrease in renal citrate content, in each of the studied renal area.

A new device for differentiated tissue sampling in rat kidney.

Since freeze-clamping does not allow to sample the diffrent renal areas a new method of kidney tissue sampling in anesthetized rats is described. It consists in sectioning a slice in the middle of the rat kidney and immediately freezing. This device allows quick and accurate separation of the different renal areas, and especially of the papilla which is almost entirely sampled.

[Benign forms of multiple sclerosis : a revaluation (author's transl)].

The authors studied 38 cases of benign forms of MS, defined as those allowing a normal or nearly normal social, occupational, and family life over a "long" period of tens of years, for at least 15 years (mean : 28 years, range : 15 to 68 years).--Forms with rare relapses with long intervals between them (18 cases), and a mean period of 14 years (5 to 30) between the first and second episode.--Recurrent forms (17 cases) with frequent attacks (one or two a year), including 8 cases with no further relapses after an average period of 10 years, and 9 cases with continuation at the same rhythm for 15 to 23 years.

Effect of monofluoroacetate on renal H+ excretion in the rat.

In order to investigate the effect of monofluoroacetate (MFA) on renal H+ excretion, anesthetized rats under mannitol diuresis were given intraperitoneally MFA and some of the acido-basic status parameters were determined. Urinary pH and pCO2 did not change after MFA administration, while urinary flow rate increased. MFA induced a decrease in H+ net excretion and in ammonia excretion.

Effect of phosphate buffer concentration on the heat resistance of Bacillus stearothermophilus spores suspended in parenteral solutions.

The effect of various quantities of Butterfield phosphate buffer added to four parenteral solutions on the survival of Bacillus stearothermophilus spores heated at 121 degrees C was determined. The effect of the addition of phosphate buffer on spore survival varied with the parenteral solution. Spore survival was increased or decreased, depending upon the composition of the parenteral solution and the buffer concentration.

[Thiamine deficiency in chronic alcoholics. Value of pre- and postoperative treatment].

Thiamine deficiency in the chronic alcoholic would appear to have a triple origin: inadequate intake, absorption and utilisation. Its consequences are well known: peripheral neuropathy, WERNICKE and KORSAKOFF type encephalopathies and cardiac problems (with asystole at the extreme). The active principle of thiamine, TPP or cocarboxylase, is involved as a coenzyme of pyruvate decarboxylase and of alphaketoglutarate decarboxylase in the ocidative decarboxylation reactions of the Krebs cycle.