[Changes in the suppressive activity of splenic lymphocytes of mice induced in vitro by sodium periodate during magnesium deficiency].

Male DBA/2 Mice were kept on a magnesium deficient diet or a control diet. The antibody response of spleen cells, after in vitro immunization with Sheep red blood cells (SRBC) was determined comparatively in the two groups of Mice, from the 19th to the 22nd day of the diet. The effect of sodium periodate treatment on the induction of in vitro antibody suppressor cells was also studies.

Changes in histamine and white blood cells in the blood, spleen and thymus of magnesium-deficient rat.

Groups of rats were given either a control or a magnesium-deficient diet. The well-known allergy-like crisis, characterized by vasodilatation with redness of the ears and dermatosis, occurred spontaneously in the magnesium-deficient groups. The histamine (H) content and the distribution of the various white blood cells (WBC) were studied as a function of time.

Effect of vitamin D deficiency on urinary excretion of connective tissue derivatives (hydroxyproline and glycosaminoglycans) in rats.

The urinary excretion of two connective tissue metabolites was studied in both control and vitamin D deficient rats. Hydroxyproline (HyPRO) excretion was determined after 2, 13 and 22 months (experiment I). It decreased with aging in animals receiving the control diet.

Magnesium deficiency allergy-like crisis in hairless rats: a suggested model for inflammation studies.

Two groups of rats, one hairy and one hairless, received either a magnesium deficient diet (4 mg Mg/100 g diet) or a control diet (40 mg/100 g diet). After four days, an easily observable redness of the skin occurred in the hairless deficient group, progressing from the tail to the forehead, and later a hyperemia of the ear and dermatosis which increased with scratching appeared in both deficient groups. The clinical signs were more acute in the deficient hairless group.

Aromatic amines (serotonin and histamine) and magnesium deficiency in the rat.

Rats were made deficient by giving a 4 mg Mg/100 g diet. The control diet content was 40 mg Mg/100 g. Serotonin injected to magnesium deficient and control rats was metabolized at the same rate in both groups and the urinary derivatives were similar.

[Blood histamine and response of spleen cells to phytohemagglutinins during moderate magnesium deficiency in the rat].

Blood histamine and spleen cell stimulation index by PHA were determined in either magnesium deficient or control Rats. Between the 10th and 17th days of diet (hyperemia and dermatosis period), histaminemia was significantly higher in deficient animals (485 ng/ml) than in control ones (112 ng/ml), but at the 32nd day it came back to normal values. The mean spleen cell stimulation index by PHA was depressed in deficient animals mainly between the 10th and 17th day of the deficiency; 33% of control mean value.

Effect of magnesium deficiency and food restriction on the immune response in young mice.

Similar batches of five-week-old C57 Bl/6 mice were given either a magnesium-deficient diet (4mg Mg/100 g), or a control diet (40 mg/100 g). Control diet intake was either ad libitum or reduced. After immunization with SRBC (sheep red blood cells), the immune response was studied by estimating the number of spleen AFC (antibody-forming cells) capable of lysing SRBC, and by a cytoadhesion test to determine the number of RFC (rosette forming cells).