Studies on the etiology of the experimental neuropathy from industrial adhesive (glues).

Chickens treated by paintbrushing with glue distillate (used in shoe industries), hydrocarbon mixture and TOCP in hexane, developed paralysis (4/5); in these, we have demonstrated a diffuse degeneration of the myelinic sheath of the peripheral and central neurites. Besides TOCP, cyclohexane (because of its higher concentration in the glue distillate and in the hydrocarbon mixture) may be indicated as responsible for occupational neuropathy.

[Research on substances with antiviral activity. VIII. Benzindanoncarboxylic acids].

New derivatives (esters, amides, nitriles, amidines, thiosemicarbazones and guanylhydrazones) of 1H-benz[e]indene-2,3-dihydro-5-methyl-1-oxo-3-carboxylic acid and 1H-benz[e]indene-2,3-dihydro-7-methyl-3-oxo-1-carboxylic acid were prepared and tested for antiviral activities in vitro against vaccinia virus, HID stock and parainfluenza type 3 virus, HA-I/CR-8 stock. Four compounds (II g), (II i), (III b), (III g), showed weak activity in vitro against vaccinia virus.

Sialidosis (mucolipidosis I).

The term "sialidosis" is suggested for the deficiency of alpha-neuraminidase activity in peripheral leukocytes and cultured fibroblasts which results in a considerable urinary excretion of sialyl-oligosaccharides. This defect was found in two siblings with a mild form of mucolipidosis I. 12 sialyl-acid rich oligosacharides have been isolated from the urine of the patients.

[Research on substances with antiviral activity. VII. Activity and lipophilic of property N-alkyl-2-chloro-3-formylindole thiosemicarbazone].

Thiosemicarbazones of N-alkyl-2-chloroindole-3-carboxaldehydes were synthesized and investigated for antiviral activity against RNA virus (parainfluenza type 3, HA-I/CR-8 stock) and DNA virus (vaccinia virus, HID stock). The effect of lipophilicity on the activity against vaccinia virus was considered. Relative lipophilicities were measured by a reversed phase thin-layer chromatographic technique.

Reactivity of alloantibodies of the Merrit B-cell system with leukemic cells and lymphoblastoid cell lines.

Twenty lymphoblastoid cell lines were typed with 38 antisera defining the 13 groups of the Merrit B-cell alloantigenic system, and results were compared with those of a 20-member CLL cell panel. While overall pattern of Merrit groups for the two types of cell population was similar, some sera in a number of groups showed unexpected negative cytotoxic reactions with lymphoid cells compared to other sera of the same group. However, in a number of instances, these negatively reacting cells could still specificity absorb out tb Merrit antibodies, suggesting that lymphoblastoid lines may show a higher incidence of the CYNAP (cytotoxicity-negative-absorption-positive) phenomenon than CLL cells.

Simplification of B-cell antisera of the Merrit system with platelets and lymphoblastoid cell lines.

Human sera with anti-HLA and anti-Merrit B-cell reactivities were absorbed with different volumes of a pool of packed outdated human platelets obtained from 237 donors. After absorption, each serum was tested against a panel of CLL cells and normal unfractionated peripheral blood lymphocytes that had given positive reactions with the same serum prior to absorption. The optimal platelet volume for complete absorption of anti-HLA and full preservation of anti-Merrit B-cell activities in these sera was found to be 1/10 to 1/5 of the volume of serum.

Distribution of HLA-A, B, C and the Merrit B-cell alloantigen specificities in chronic lymphatic leukemia.

Based upon reactions with both normal peripheral B-lymphocytes and a CLL panel, an additional 3 new specificities of the Merritt B-cell alloantigenic system have been defined. this brings the number of provisionally designated specificities to 22. The system of antigenic determinants is well represented on normal B-lymphocytes, and has a roughly similar distribution when compared to the CLL panel; however, evidence is presented which suggests that certain speficities may have significantly idfferent distributions on the 2 different panels, but the number of cells tested is low.

New groups and segregant series among B-cell alloantigens of the Merrit system. A study of leukemia cells, peripheral B cells, and lymphoblastoid cell lines.

On the basis of reactions with a chronic lymphatic leukemia cell panel, evidence for 6 new specificities of the Merrit B-cell alloantigenic system in man is presented, bringing the number of provisionally defined specificities to 19. These can be roughly divided into two segregant series. The system is well represented on both homozygous and heterozygous B-type lymphoblastoid cell lines.

The merrit alloantigenic system of human B lymphocytes: evidence for thirteen possible factors including one six-member segregant series.

An analysis of the typing results of a 70-member chronic lymphatic leukemia B cell panel revealed evidence for 13 possible groups of the Merrit alloantigenic system. Six of these appeared possibly allelic and may represent a segregant series. The CLL panel was also fully typed for HLA and some degree of linkage dysequilibrium between Merrit and HLA seemed apparent from the data.

Human alpha-fucosidase. Single residual enzymatic form in fucosidosis.

Four major forms of alpha-fucosidase (EC 3.2.1.

Comparison of the allospecific and viral-specific immune responses to irradiated versus formaldehyde-fixed allogeneic Moloney lymphoma cells in CBA mice.

Two groups of adult CBA mice were immunized with 10-7 allogeneic Moloney lymphoma (YAC) cells. These YAC (H-2a) cells, which were either irradiated with 6000 R (Group i) or were formaldehyde fixed (Group II), were injected i.p.

Possible use of established cell lines for MLR locus typing.

MLR histocompatibility typing was performed by in vitro stimulation of lymphocytes from various persons with fresh lymphocytes (irradiated) or with lymphoblostoid cell lines (mito-mycin-treated) derived from donors homozygous at the MLR locus. MLC responses to both types of stimulation correlated strongly suggesting that a) established lymphoblastoid line cells can express products of the MLR loc and b) fresh lymphocytes can be replaced by these long term lines in MLR typing. Two lines (LV-B and MS-B) derived from two MLR homozygous donors (lv and MS) were used throughout these experiments.