CHARACTERIZATION OF CENTROMERIC HISTONE H3 VARIANTS IN ALLOPOLYPLOIDS OF WHEAT AND RYE.

Chromosome elimination of one parental species in hybrid cell observed even after successful fertilization is one of a common phenomenon and the main problems of remote hybridization. Centromeres regulate the process of faithful segregation of chromosomes during cell division. Constant component of the centromeric chromatin is a specialized histone H3 modification (CENH3).

VARIABILITY OF CENTROMERIC HISTONE H3 VARIANTS AND PHYLOGENETIC RELATIONSHIPS IN SECALE.

We examined nucleotide sequence variation in centromeric histone H3 (CENH3) in fifteen rye species and subspecies, including annuals, perennials, self-pollinating and cross-pollinated plants. Levels of genetic variation within N-terminal tail (NTT) and histone fold domain (HFD) were estimated as average per site pairwise nucleotide diversity (p) and as the number of segregating polymorphic sites (S). A comparison of nucleotide diversity (ptot) for NTT and HFD showed that estimates of diversity are consistently greater in NTT.