Publications by authors named "Garzella P"

Objectives: to perform cross-cultural adaptation, face and content validation and reliability analysis of the Medication Safety Thermometer tool for use in Brazil.

Methods: the process of cross-cultural adaptation and validation followed the stages of translation, synthesis of translations, back-translation, content validation performed by experts, and face validation through pre-testing. Reliability was determined by calculating the Kappa coefficient.

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Biofilms and infectious process may alter free antimicrobial concentrations at the site of infection. Tobramycin (TOB), an aminoglycoside used to treat lung infections caused by Pseudomonas aeruginosa, binds to alginate present in biofilm extracellular matrix increasing its minimum inhibitory concentration (MIC). This work aimed to investigate the impact of biofilm-forming P.

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A class of implantable, regeneration-type neural interfaces (NI's) for mammalian peripheral nerve recording and stimulation were developed using different fabrication processes and integrating purposely designed components. A typical NI comprises three main components: 1) a microfabricated silicon die incorporating a microelectrode array on multiple through-holes, 2) a polymer guidance channel housing the die, and 3) a flexible flat cable connecting the die to an external electronic circuitry. The design and fabrication of the NI's were aimed at achieving long term, reliable implants by taking into careful account the biological, electrical, and mechanical requirements of the specific implant site.

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1. Passive force responses to ramp stretches at various velocities were measured in intact and skinned single muscle fibres isolated from the lumbricalis muscle of the frog. Force was measured using a fast capacitance transducer and sarcomere length was measured using a laser light diffraction technique at a point very close to the fixed end so as to avoid effects of fibre inertia.

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1. Force responses to ramp stretches were recorded in single muscle fibres isolated from the lumbricalis muscle of the frog. Stretches were applied at rest and at progressively increasing times after a single stimulus.

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The possibility that weakly binding bridges are attached to actin in the absence of Ca2+ under physiological conditions was investigated by studying the force response of unstimulated intact muscle fibres of the frog to fast ramp stretches. The force response during the stretching period is divided into two phases: phase 1, coincident with the acceleration period of the sarcomere length change and phase 2, synchronous with sarcomere elongation at constant speed. The phase 1 amplitude increases linearly with the stretching speed in all the range tested, while phase 2 increases with the speed but reaches a plateau level at about 50 x 10(3) nm/half sarcomere per second.

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The effects of 2,3-butanedione monoxime (BDM) on contraction characteristics were studied at 5 degrees C in single intact fibres isolated from the tibialis anterior muscle of the frog. The force-velocity relation was determined using the controlled-velocity method in either whole fibres or short fibre segments in which sarcomere shortening was measured by a laser light diffraction method. It is shown that 3 mM BDM decreases the speed of rise and the amount of tetanus tension, reduces the maximum velocity of shortening and increases the curvature of the force-velocity relation, as well as the value for the stiffness to tension ratio.

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